LOCUS       BBa_K618211             1225 bp    DNA     linear       11-May-2024
DEFINITION  pLAC ompA-Gli1
ACCESSION   BBa_K618211
VERSION     BBa_K618211.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     misc_feature    229..235
                     /label=NdeI
     CDS             232..666
                     /label=OmpA
     CDS             667..692
                     /label=linker
     misc_feature    687..692
                     /label=SacI
     misc_feature    232..234
                     /label=ATG
     misc_feature    229..692
                     /label=BBa_K103006
     misc_feature    701..1225
                     /label=BBa_K165007
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagagca tatgaaagct
      241 actaaactgg tactgggcgc ggtaatcctg ggttctactc tgctggcagg ttgctccagc
      301 aacgctaaaa tcgatcaggg aattaacccg tatgttggct ttgaaatggg ttacgactgg
      361 ttaggtcgta tgccgtacaa aggcagcgtt gaaaacggtg catacaaagc tcagggcgtt
      421 caactgaccg ctaaactggg ttacccaatc actgacgacc tggacatcta cactcgtctg
      481 ggtggcatgg tatggcgtgc agacactaaa tccaacgttt atggtaaaaa ccacgacacc
      541 ggcgtttctc cggtcttcgc tggcggtgtt gagtacgcga tcactcctga aatcgctacc
      601 cgtctggaat accagtggac caacaacatc ggtgacgcac acaccatcgg cactcgtccg
      661 gacaacggcg gaggttctgg aggagggagc tctactagag aagcgtgagc ctgaatctgt
      721 gtatgaaact gactgccgtt gggatggctg tagccaggaa tttgactccc aagagcagct
      781 ggtgcaccac atcaacagcg agcacatcca cggggagcgg aaggagttcg tgtgccactg
      841 ggggggctgc tccagggagc tgaggccctt caaagcccag tacatgctgg tggttcacat
      901 gcgcagacac actggcgaga agccacacaa gtgcacgttt gaagggtgcc ggaagtcata
      961 ctcacgcctc gaaaacctga agacgcacct gcggtcacac acgggtgaga agccatacat
     1021 gtgtgagcac gagggctgta gtaaagcctt cagcaatgcc agtgaccgag ccaagcacca
     1081 gaatcggacc cattccaatg agaagccgta tgtatgtaag ctccctggct gcaccaaacg
     1141 ctatacagat cctagctcgc tgcgaaaaca tgtcaagaca gtgcatggtc ctgacgccca
     1201 tgtgaccaaa cggcaccgtg gggat
//