LOCUS       BBa_K618212              952 bp    DNA     linear       17-May-2024
DEFINITION  pLAC ompA-zif268
ACCESSION   BBa_K618212
VERSION     BBa_K618212.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     misc_feature    229..235
                     /label=NdeI
     CDS             232..666
                     /label=OmpA
     CDS             667..692
                     /label=linker
     misc_feature    687..692
                     /label=SacI
     misc_feature    232..234
                     /label=ATG
     misc_feature    229..692
                     /label=BBa_K103006
     misc_feature    701..952
                     /label=BBa_K165006
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagagca tatgaaagct
      241 actaaactgg tactgggcgc ggtaatcctg ggttctactc tgctggcagg ttgctccagc
      301 aacgctaaaa tcgatcaggg aattaacccg tatgttggct ttgaaatggg ttacgactgg
      361 ttaggtcgta tgccgtacaa aggcagcgtt gaaaacggtg catacaaagc tcagggcgtt
      421 caactgaccg ctaaactggg ttacccaatc actgacgacc tggacatcta cactcgtctg
      481 ggtggcatgg tatggcgtgc agacactaaa tccaacgttt atggtaaaaa ccacgacacc
      541 ggcgtttctc cggtcttcgc tggcggtgtt gagtacgcga tcactcctga aatcgctacc
      601 cgtctggaat accagtggac caacaacatc ggtgacgcac acaccatcgg cactcgtccg
      661 gacaacggcg gaggttctgg aggagggagc tctactagag cccttccagt gtcgcatttg
      721 catgcggaac ttttcgctca ggacggacct tgacaggcat acccgtactc ataccggtga
      781 aaaaccgttt cagtgtcgga tctgtatgcg aaatttctcc ctcagccaga ccttgcgccg
      841 ccatctacgt acgcacaccg gcgagaagcc attccaatgc cgaatatgca tgcgcaactt
      901 cagtctcagg agcaacctgg ggcggcacct aaaaacccac acaggagaaa aa
//