BBa_K619894
1
BBa_K619894
mutated prfA-UTR thermosensor from Listeria monocytogenes
2011-09-26T11:00:00Z
2015-05-08T01:12:54Z
prfA-UTR from Listeria monocytogenes
This is the prfA-UTR thermosensor mutated to produce a thermosensor with a increased expression at both 30 and 37 degress when compared with wild-type. One can still see induction when switching from 30 to 37 degrees. Characterization of its dynamic range is underway. The part was made by TAQ-mediated random mutagenesis of our "wild-type" thermosensor (BBa-19889) and subsequent screening using a lacZ reporter. There are other thermosensors entered into the IGEM database with different dynamic ranges obtained from this screen. To use this thermosensor, be sure to clone in frame with the indicated initiating ATG.
false
false
_791_
0
8978
9
It's complicated
false
Our "wild-type" thermosensor (BBa-19889) Shine-Delagarno has been optimized for expression in E. coli. Otherwise, the "wild-type" sequence is identical to the thermosensor sequence from Listeria monocytogenes.
false
Julianne Grose
annotation2144644
1
start
range2144644
1
125
127
annotation2144643
1
rbs
range2144643
1
113
118
BBa_K619894_sequence
1
tgcagtttagcgtgcggctgcagtttagcgtgactttctttcaacagctaacaattgttgttactgcctaatgtttttagggtattttaaaaaagggcgataaaaaacgattggaggatgagacatgaacgctcaaaagc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z