BBa_K619898
1
BBa_K619898
medium length prfA-UTR thermosensor from Lysteria Monocytogenes
2011-09-27T11:00:00Z
2015-05-08T01:12:55Z
This is a truncated prfA-UTR from Listeria monocytogenes made by annealing primers with the desired sequence.
This is a truncation of prfA-UTR thermosensor with the middle section of the hairpin removed (see diagram). It does not respond to temperature shifts of 30 to 37 degrees. This is part of a collection of thermosensors where the parent prfA-UTR from Listeria monocytogenes has been mutated (either by design or random mutagenesis). The parent thermosensor is BBa_K619889.
false
false
_791_
0
8978
9
Not in stock
false
Our "wild-type" thermosensor (BBa-19889) Shine-Delgarno has been optimized for expression in E. coli. Otherwise, the "wild-type" sequence is identical to the thermosensor sequence from Listeria monocytogenes.
false
Julianne Grose
annotation2146065
1
start
range2146065
1
84
86
annotation2146064
1
rbs
range2146064
1
72
77
BBa_K619898_sequence
1
tttagcgtgactttctttcaacagctaacaattgttgttactgcctaatgtaagggcgataaaaaacgattggaggatgagacatgaacgctcaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z