BBa_K620000
1
BBa_K620000
DDT Dehydrochlorinase
2011-09-19T11:00:00Z
2015-05-08T01:12:55Z
This part was isolated from Anopheles dirus. The team took the amino acid sequence found in the NCBI protein database and used reverse transcribed using codon optimization for E. coli to create the DNA part.
Released HQ 2013
Also known as Glutathione S-transferase 1-1, this protein is supposed to degrade DDT, an endocrine disruptor and persistent organic pollutant.
false
false
_792_
0
9568
9
In stock
true
The part was assembled through PCR using a set of long oligos. The sequence contains an NdeI site, which made it more difficult to place into a pET11a vector for protein purification and characterization.
false
Caltech iGEM 2011
annotation2135663
1
double stop codon
range2135663
1
628
633
annotation2135664
1
K620000
range2135664
1
1
633
BBa_K620000_sequence
1
atggacttttactatctgccgggctcagctccgtgtcgtgcggtccaaatgacggcagcggcggttggagtggaactgaatttgaaactgaccgatctgatgaaaggtgaacatatgaaaccggagttccttaagctgaacccccagcattgcattcccaccctggtcgacaatggatttgccctgtgggagtctcgtgcgattcagatttatctggcggagaaatatggcaaagatgataaactgtatccgaaagatccacagaaacgtgctgtggttaatcagcggctgtattttgatatgggaaccctgtatcagcgtttcgccgattatcattatccgcagatttttgctaaacaaccggctaatccggaaaacgaaaagaaaatgaaggatgcggtgggatttctgaatacctttctggaaggccaggaatatgctgctggcaatgatttaaccatcgcggatctgagcctggcggcgaccattgcgacctatgaggtggcaggctttgatttcgcgccgtatcctaacgttgcggcttggttcgcgcgttgcaaagcgaatgctcctggatatgcgctgaatcaggctggtgcagatgagtttaaagctaaattcctgagctagtag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z