BBa_B0011
1
BBa_B0011
LuxICDABEG (+/-)
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
Released HQ 2013
Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p>
false
false
_1_
0
24
7
In stock
false
<P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P>
true
Reshma Shetty
annotation1683
1
stem_loop
range1683
1
13
35
annotation7019
1
BBa_B0011
range7019
1
1
46
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_R0010
1
LacI
promoter (lacI regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
false
true
_1_
0
24
7
In stock
false
<P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
true
annotation1961222
1
BBa_R0010
range1961222
1
1
200
annotation1961226
1
LacI binding site
range1961226
1
166
200
annotation1961221
1
end of LacI coding region (inactive)
range1961221
1
1
88
annotation1961223
1
CAP binding site
range1961223
1
89
126
annotation1961224
1
-35
range1961224
1
137
142
annotation1961227
1
start
range1961227
1
173
173
annotation1961225
1
-10
range1961225
1
161
166
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
BBa_K123000
1
BisdA
BisdA
2008-10-27T12:00:00Z
2015-05-08T01:09:44Z
Sphingomonas sp. strain AO1
When used in conjunction with BisdB this enzyme can be used to degrade Bisphenol A. Activity has previously been recorded in E.coli by Miho Sasaki1 et al. 2005.
Miho Sasaki1, Jun-ichi Maki, Ko-ichi Oshiman, Yoshinobu Matsumura1, and Tetsuaki Tsuchido.2005,
Biodegradation of bisphenol A by cells and cell lysate from Sphingomonas sp. strain AO1. Biodegredation 16: 449-459
false
false
_241_
0
2742
9
It's complicated
true
These parts where designed to include a his tag to make it easier to view enzyme presence
true
Jason Gardiner
BBa_K620002
1
BBa_K620002
LacI promoter + BisdA
2011-09-19T11:00:00Z
2015-05-08T01:12:55Z
Assembled from BBa_R0010, BBa_K123000, BBa_B0014.
Allows for inducible expression of the protein BisdA, one of a pair of proteins that can degrade bisphenol A (BPA).
false
false
_792_
0
9568
9
It's complicated
false
None.
false
Caltech iGEM 2011
component2145161
1
BBa_B0014
component2145153
1
BBa_B0034
component2145154
1
BBa_K123000
component2145145
1
BBa_R0010
annotation2145154
1
BBa_K123000
range2145154
1
213
542
annotation2145161
1
BBa_B0014
range2145161
1
543
637
annotation2145145
1
BBa_R0010
range2145145
1
1
200
annotation2145153
1
BBa_B0034
range2145153
1
201
212
BBa_B0014
1
BBa_B0014
double terminator (B0012-B0011)
2003-07-15T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0012 and BBa_B0011
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component939303
1
BBa_B0012
component939311
1
BBa_B0011
annotation939311
1
BBa_B0011
range939311
1
50
95
annotation939303
1
BBa_B0012
range939303
1
1
41
BBa_R0010_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
BBa_B0014_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K123000_sequence
1
atggccggccctcatatccaagtgactacccgcgatggggagatacgcgaactcgacgtcgcagcctccgggttcctgatggaagcccttcgcgacgccaatatcgacggcgtcgaggcgatgtgcggcggatgctgctcctgcgcgacctgccacgtctacatcgacgctgctcccgccgggaccctgccgccggtctcctccgacgaggagatgctgctttccggcctggtctcgacccccggacggtcgcggctctcctgccaaattccggtcacggccgaactggatggccttaagctcacgatcccgccggactccaccggttaa
BBa_B0011_sequence
1
agagaatataaaaagccagattattaatccggcttttttattattt
BBa_K620002_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaaaagaggagaaaatggccggccctcatatccaagtgactacccgcgatggggagatacgcgaactcgacgtcgcagcctccgggttcctgatggaagcccttcgcgacgccaatatcgacggcgtcgaggcgatgtgcggcggatgctgctcctgcgcgacctgccacgtctacatcgacgctgctcccgccgggaccctgccgccggtctcctccgacgaggagatgctgctttccggcctggtctcgacccccggacggtcgcggctctcctgccaaattccggtcacggccgaactggatggccttaagctcacgatcccgccggactccaccggttaatcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z