LOCUS       BBa_K620003             1445 bp    DNA     linear       22-Nov-2024
DEFINITION  Tet promoter + BisdB
ACCESSION   BBa_K620003
VERSION     BBa_K620003.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    59..62
                     /label=conserved
     RBS             55..66
                     /label=BBa_B0034
     CDS             67..1350
                     /label=BBa_K123001
     stem_loop       1358..1377
                     /label=T7 TE
     misc_feature    1384..1384
                     /label=stop
     polyA_site      1378..1391
                     /label=polya
     misc_feature    1351..1391
                     /label=BBa_B0012
     terminator      1351..1391
                     /label=BBa_B0012
     stem_loop       1412..1434
                     /label=stem_loop
     misc_feature    1400..1445
                     /label=BBa_B0011
     terminator      1400..1445
                     /label=BBa_B0011
     terminator      1351..1445
                     /label=BBa_B0014
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacaaagag
       61 gagaaaatgg ccggcaaccc tcagacactg ccggtctttc cagacctcga catcttttcg
      121 cccgaatatg cctgcaaccg cgaaaaatac gcagcccgcg cccttaggga ctatccgctg
      181 cacttctata agccgctgaa tttgtggatc gtcagcaagc acaaggatgt gcgctcggcc
      241 ttgttcactc cgcaggtctt ttcttctgtc gcgtttggcc tgctcccccc gcccgatgat
      301 atcgcgccgc gcgtgccgga cctttacacc gacgtgcatc tgccctcgat ggacccgccg
      361 gagcacacca agctcagggt tcccgtccag caagccctcc tccccggtcg gctggtgggt
      421 aaggacgagg tcgttcgccg gatcgccaac gaacttatcg ataccttcat cgacaagggc
      481 gaatgcgatc tcctccacga tttcagctac aaacttgcgc tctacttgat cgtcgatctg
      541 ctcgggatcc ccaaggaacg ggcggaagat tatcatcgct ggtccaactg cttcttccag
      601 ctgttcaccc ctaaggtacc ggagcgcgcc gacgcaagat tcttcgtgcc gatgccggaa
      661 gaggtactgc ggcagaactg ggaaggcctg gcggaggcga acgactacct gcgcgaagtc
      721 gtcgagaacc ttgatcgcaa tcccggcaac aacatgctgt caaacctgtt gcagctgcgc
      781 gaaccggacg gctcacgcac gatcacgatc agcgccaatg tttgcaacgc actggaattt
      841 ggggcggctg gtcatgacac cacggcaacg ctgatcgcgc acctcaccta tttcgtgctg
      901 accacgccgg acctcaagga cactcttacc gaggatccgt cgctgatccc cgcggcgatt
      961 tccgaaacgc tgcgccgtcg ctgtccggtc gacgggctgt tccggcgaac gctgtcggat
     1021 gtagaactat gcggccagaa aatcgaatcc ggatcgatcg tctacctcga tctcaccgct
     1081 gcaaatcttg accccgatgt attccccgaa cctgagacgt ttcgcctgaa ccgcgacaat
     1141 atcaaggaaa tggtctcttt cggcttcggt cgtcacgtct gcgcaggaca gtacctgtcc
     1201 cgtatcgaag caaaagcggc ctacgaagaa ctgatgcgcc gtattcccaa catgcgtctc
     1261 gctgatggtt tcaagctgga atacatgcct tccgtggcga cgacggttct caaggggctt
     1321 ccgctggtct gggacaaaaa caccggttaa tcacactggc tcaccttcgg gtgggccttt
     1381 ctgcgtttat atactagaga gagaatataa aaagccagat tattaatccg gcttttttat
     1441 tattt
//