##gff-version 3
##sequence-region BBa_K624001 1 1571
BBa_K624001	.	engineered_region	694	1571	.	+	0	ID=BBa_E0840;Name=BBa_E0840
BBa_K624001	.	ribosome_entry_site	694	708	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E0840
BBa_K624001	.	engineered_region	694	708	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K624001	.	ribosome_entry_site	694	708	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K624001	.	ribosome_entry_site	701	705	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K624001	.	CDS	715	1434	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0840
BBa_K624001	.	CDS	715	1434	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K624001	.	terminator	1443	1522	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0840
BBa_K624001	.	stem_loop	1454	1497	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K624001	.	engineered_region	1443	1522	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K624001	.	terminator	1531	1571	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0840
BBa_K624001	.	engineered_region	1531	1571	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K624001	.	stop_codon	1564	1564	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K624001	.	polyA_site	1558	1571	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K624001	.	stem_loop	1538	1557	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K624001	.	CDS	1	685	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_K624001	.	tag	621	654	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K624001	.	engineered_tag	661	685	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K624001	.	CDS	4	620	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
>BBa_K624001
atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaa
cccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcga
cgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagatttttta
cgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggccta
cagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattata
tgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagg
gaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccag
ccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacga
cgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagattaaagaggagaaatac
tagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcaca
aattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaa
actacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatg
aaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacggga
actacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaa
agaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaa
aagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaac
aaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaaga
tcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaacta
tacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgt
tgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata