LOCUS       BBa_K624002             1643 bp    DNA     linear       12-May-2024
DEFINITION  pT7+RBS+tetR+RBS+GFP+Ter
ACCESSION   BBa_K624002
VERSION     BBa_K624002.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=BBa_I712074
     misc_feature    59..62
                     /label=conserved
     RBS             55..66
                     /label=BBa_B0034
     CDS             76..692
                     /label=tetR
     misc_feature    693..726
                     /label=SsrA
     misc_feature    733..757
                     /label=Help:Barcodes
     CDS             73..757
                     /label=BBa_C0040
     RBS             766..780
                     /label=RBS-1\Strong
     RBS             773..777
                     /label=RBS
     misc_feature    766..780
                     /label=BBa_B0030
     RBS             766..780
                     /label=BBa_B0030
     CDS             787..1506
                     /label=GFP protein
     CDS             787..1506
                     /label=BBa_E0040
     stem_loop       1526..1569
                     /label=stem_loop
     misc_feature    1515..1594
                     /label=BBa_B0010
     terminator      1515..1594
                     /label=BBa_B0010
     stem_loop       1610..1629
                     /label=T7 TE
     misc_feature    1636..1636
                     /label=stop
     polyA_site      1630..1643
                     /label=polya
     misc_feature    1603..1643
                     /label=BBa_B0012
     terminator      1603..1643
                     /label=BBa_B0012
     misc_feature    766..1643
                     /label=BBa_E0840
     CDS             73..1643
                     /label=BBa_K624001
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcatact agagaaagag
       61 gagaaatact agatgtccag attagataaa agtaaagtga ttaacagcgc attagagctg
      121 cttaatgagg tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta
      181 gagcagccta cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc
      241 attgagatgt tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa
      301 gattttttac gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga
      361 gcaaaagtac atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa
      421 ttagcctttt tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgct
      481 gtggggcatt ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa
      541 gaaagggaaa cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta
      601 tttgatcacc aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga
      661 ttagaaaaac aacttaaatg tgaaagtggg tccgctgcaa acgacgaaaa ctacgcttta
      721 gtagcttaat aacactgata gtgctagtgt agatcactac tagagattaa agaggagaaa
      781 tactagatgc gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta
      841 gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca
      901 tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca
      961 acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc agatcatatg
     1021 aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata
     1081 tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc
     1141 cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga
     1201 cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag
     1261 aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta
     1321 gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac
     1381 cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg
     1441 gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa
     1501 taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     1561 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
     1621 gggtgggcct ttctgcgttt ata
//