LOCUS BBa_K624017 3317 bp DNA linear 04-May-2024 DEFINITION pYMB essentials+[Y-13-R]+[CHAMP] ACCESSION BBa_K624017 VERSION BBa_K624017.1 FEATURES Location/Qualifiers misc_feature 1..80 /label=ori (pMGT) promoter 81..274 /label=Pmsp1 misc_feature 275..277 /label=start promoter 1..277 /label=BBa_K624000 CDS 313..1142 /label=rep (pMGT) RBS 286..309 /label=rbs from pMGT misc_feature 310..312 /label=start CDS 286..1142 /label=BBa_K624003 CDS 1..1142 /label=BBa_K624004 RBS 1151..1178 /label=rbs (from Pmsp3) misc_feature 1179..1181 /label=artificial start codon RBS 1151..1181 /label=BBa_K624012 misc_feature 1188..1190 /label=start CDS 1191..1904 /label=EYFP CDS 1905..2276 /label=mms13 CDS 2277..3209 /label=rLuc misc_feature 3210..3212 /label=stop CDS 1188..3212 /label=BBa_K624006 RBS 3221..3248 /label=rbs (from Pmsp3) misc_feature 3249..3251 /label=artificial start codon RBS 3221..3251 /label=BBa_K624012 misc_feature 3258..3260 /label=start misc_feature 3315..3317 /label=stop CDS 3261..3314 /label=CHAMP (anti-H1[mms13]) CDS 3258..3317 /label=BBa_K624010 ORIGIN 1 tgatccgcca gcgccgaagc catcataact gcattgaata attcaaagtg cattgaataa 61 ggcagggtgc ataaggcgca gcggtaacgc cgcctcgccg gtggaagggt gttgtgtggc 121 atccatgcca cagtgcggag ttttcgatac agccgacgtc ccgcctcgct tgactggcgg 181 cgcccatcat ggcgtgatgg cgaccgttcg ttcatttgga tagcggtcgc ggccattggg 241 ggtcgttttc gcaaagccaa agagaggaga taccatgtac tagagcggga gagggagctg 301 atcccgctga tggtgacgct gacaggccat cacaatgcac ggatgagcct cgctgaatgt 361 ctgacggtga tgaagaaggc caagcagagc cttcaccagt ctggcccatg gaagcgccat 421 gttggcccgg tggtcgaagg gcacgtcacg gcaacggagg tcaatcggtc acgccgccat 481 ggctggcacg tccattttca cgtcctgatg ctgattgagg ccgccagcga ggccgaagcg 541 attgcgctcg tgacccgaca cctcgaacct gaatggctcc ggcagcttga gcgccaaggc 601 agctggggcg gcgaacatgc gttagacgtc cagggcgcag ccgatgcgga tcggtatgtg 661 gccaagtggg gcgcggctga ggaattggct ctgtcgggcg aaaagcgggc gcgcggcgac 721 gccgaaatta agggtcaaac gccgtgggaa ttgttggccg ctgcggctga tggtgacgtc 781 caggctggcc gcgattttgt ggagtacgcg accctgtttc atggcaaacg ccagctcgtt 841 tggtcgcgag gactgaaaga gcgtgtcggc ctcaaggtgg tggaagacga cgaggccgcc 901 ggcgagctgg aggatgtcgc agatagcgcc atcaacgaag tggccgtgtt gccgaggccg 961 acttggcgtc acatcgtccg gcgcaggctc cgcgcggatc tgctggccgc cgctcgtgat 1021 gacggattcg atggcgtcgc gcggctgctc aatgagcatg gcatcggcgg agctatgccc 1081 ggcggctggc gaccaatgct tagcgaatat accgagcata ttgcgatggc tgcgtgactc 1141 tttactagag gtccaagccc tatgagggag cctttctaat gtactagatg gtgagcaagg 1201 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg 1261 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc 1321 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct 1381 tcggctacgg cctgcaatgc ttcgcccgct accccgacca catgaagctg cacgacttct 1441 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg 1501 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg 1561 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca 1621 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga 1681 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc 1741 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc 1801 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg 1861 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagatgccc tttcaccttg 1921 ccccctatct ggcgaaatcc gttcccggcg tcggcgttct cggcgccctg gtcggcggcg 1981 ccgccgcctt ggccaagaac gtccgcctcc tgaaggaaaa gcgcatcacc aataccgaag 2041 cggccatcga taccggcaag gaaaccgtcg gcgccggcct ggccaccgcg ctttccgccg 2101 tggccgcgac cgccgtcggc ggcggcctgg ttgtatcgct gggcaccgcc ttggtggccg 2161 gcgttgccgc caaatatgcc tgggatcgcg gcgtcgatct ggtcgagaag gaactgaacc 2221 gcggcaaagc tgccaacggc gcttccgacg aggacatctt gcgggacgaa ctggccatgg 2281 cttccaaggt gtacgacccc gagcaacgca aacgcatgat cactgggcct cagtggtggg 2341 ctcgctgcaa gcaaatgaac gtgctggact ccttcatcaa ctactatgat tccgagaagc 2401 acgccgagaa cgccgtgatt tttctgcatg gtaacgctgc ctccagctac ctgtggaggc 2461 acgtcgtgcc tcacatcgag cccgtggcta gatgcatcat ccctgatctg atcggaatgg 2521 gtaagtccgg caagagcggg aatggctcat atcgcctcct ggatcactac aagtacctca 2581 ccgcttggtt cgagctgctg aaccttccaa agaaaatcat ctttgtgggc cacgactggg 2641 gggcttgtct ggcctttcac tactcctacg agcaccaaga caagatcaag gccatcgtcc 2701 atgctgagag tgtcgtggac gtgatcgagt cctgggacga gtggcctgac atcgaggagg 2761 atatcgccct gatcaagagc gaagagggcg agaaaatggt gcttgagaat aacttcttcg 2821 tcgagaccat gctcccaagc aagatcatgc ggaaactgga gcctgaggag ttcgctgcct 2881 acctggagcc attcaaggag aagggcgagg ttagacggcc taccctctcc tggcctcgcg 2941 agatccctct cgttaaggga ggcaagcccg acgtcgtcca gattgtccgc aactacaacg 3001 cctaccttcg ggccagcgac gatctgccta agatgttcat cgagtccgac cctgggttct 3061 tttccaacgc tattgtcgag ggagctaaga agttccctaa caccgagttc gtgaaggtga 3121 agggcctcca cttcagccag gaggacgctc cagatgaaat gggtaagtac atcaagagct 3181 tcgtggagcg cgtgctgaag aacgagcagt aatactagag gtccaagccc tatgagggag 3241 cctttctaat gtactagatg aagaagctgt tcgtgctgct gatgttcatc atcgtggccc 3301 tgctgtggaa gaagtaa //