pYMB indicates pUC19 with ori+Pmsp1+rep. In this construct, 13-R and Y-CHAMP are ligated in the downstream of ori+Pmsp1+rep, aimed at serving as optogenetic wireless neurostimulator with OFF state (no BRET between EYFP and rLuc) as default mode. This design is made in case EYFP fusion on N-terminal of mms13 has negative effects on its folding.
mms13 from genomic DNA of Magnetospirillum magneticum AMB-1
alternative design for optogenetic wireless neurostimulation preventing blockade of signaling peptide located on helix 1 of mms13
synthetic CHAMP and fusion efforts on fusing EYFP on the N-terminal
CHAMP carrying EYFP at N-terminal, targeting helix 1 of mms13
predicted RBS site from Pmsp3, the strongest promoter of Magnetospirillum magneticum AMB-1
only the plasmid contain rep gene can be used in magnetic bacteria AMB-1
Conversion of the iGEM parts registry to SBOL2.1