LOCUS       BBa_K624024             2927 bp    DNA     linear       22-Nov-2024
DEFINITION  pYMB essentials+[YN-13-R]
ACCESSION   BBa_K624024
VERSION     BBa_K624024.1  
FEATURES             Location/Qualifiers
     misc_feature    1..80
                     /label=ori (pMGT)
     promoter        81..274
                     /label=Pmsp1
     misc_feature    275..277
                     /label=start
     promoter        1..277
                     /label=BBa_K624000
     CDS             313..1142
                     /label=rep (pMGT)
     RBS             286..309
                     /label=rbs from pMGT
     misc_feature    310..312
                     /label=start
     CDS             286..1142
                     /label=BBa_K624003
     CDS             1..1142
                     /label=BBa_K624004
     misc_feature    1149..1151
                     /label=start
     CDS             1152..1616
                     /label=YN (EYFP)
     CDS             1617..1991
                     /label=mms13
     CDS             1992..2924
                     /label=rLuc
     misc_feature    2925..2927
                     /label=stop
     CDS             1149..2927
                     /label=BBa_K624008
ORIGIN
        1 tgatccgcca gcgccgaagc catcataact gcattgaata attcaaagtg cattgaataa
       61 ggcagggtgc ataaggcgca gcggtaacgc cgcctcgccg gtggaagggt gttgtgtggc
      121 atccatgcca cagtgcggag ttttcgatac agccgacgtc ccgcctcgct tgactggcgg
      181 cgcccatcat ggcgtgatgg cgaccgttcg ttcatttgga tagcggtcgc ggccattggg
      241 ggtcgttttc gcaaagccaa agagaggaga taccatgtac tagagcggga gagggagctg
      301 atcccgctga tggtgacgct gacaggccat cacaatgcac ggatgagcct cgctgaatgt
      361 ctgacggtga tgaagaaggc caagcagagc cttcaccagt ctggcccatg gaagcgccat
      421 gttggcccgg tggtcgaagg gcacgtcacg gcaacggagg tcaatcggtc acgccgccat
      481 ggctggcacg tccattttca cgtcctgatg ctgattgagg ccgccagcga ggccgaagcg
      541 attgcgctcg tgacccgaca cctcgaacct gaatggctcc ggcagcttga gcgccaaggc
      601 agctggggcg gcgaacatgc gttagacgtc cagggcgcag ccgatgcgga tcggtatgtg
      661 gccaagtggg gcgcggctga ggaattggct ctgtcgggcg aaaagcgggc gcgcggcgac
      721 gccgaaatta agggtcaaac gccgtgggaa ttgttggccg ctgcggctga tggtgacgtc
      781 caggctggcc gcgattttgt ggagtacgcg accctgtttc atggcaaacg ccagctcgtt
      841 tggtcgcgag gactgaaaga gcgtgtcggc ctcaaggtgg tggaagacga cgaggccgcc
      901 ggcgagctgg aggatgtcgc agatagcgcc atcaacgaag tggccgtgtt gccgaggccg
      961 acttggcgtc acatcgtccg gcgcaggctc cgcgcggatc tgctggccgc cgctcgtgat
     1021 gacggattcg atggcgtcgc gcggctgctc aatgagcatg gcatcggcgg agctatgccc
     1081 ggcggctggc gaccaatgct tagcgaatat accgagcata ttgcgatggc tgcgtgactc
     1141 tttactagat ggtgtccaag ggcgaggaac tgttcaccgg cgtggtgccc atcctggtgg
     1201 agctggacgg cgacgtgaac ggccacaagt tcagcgtgtc cggcgagggc gaaggcgacg
     1261 ccacctacgg caagctgacc ctgaagctga tctgcaccac cggcaagctg cccgtgccct
     1321 ggcccaccct ggtgaccacc ctgtacctcc agtgcttcgc cagatacccc gaccacatga
     1381 agcagcacga tttcttcaag agcgccatgc ccgagggcta cgtgcaggaa cggaccatct
     1441 tcttcaagga cgacggcaac tacaagacca gagccgaagt gaagttcgag ggcgacacac
     1501 tggtgaaccg gatcgagctg aagggcatcg acttcaaaga ggacggcaat atcctgggcc
     1561 acaagctgga atacaactac aacagccaca acgtgtacat caccgccgac aagcagatgc
     1621 cctttcacct tgccccctat ctggcgaaat ccgttcccgg cgtcggcgtt ctcggcgccc
     1681 tggtcggcgg cgccgccgcc ttggccaaga acgtccgcct cctgaaggaa aagcgcatca
     1741 ccaataccga agcggccatc gataccggca aggaaaccgt cggcgccggc ctggccaccg
     1801 cgctttccgc cgtggccgcg accgccgtcg gcggcggcct ggttgtatcg ctgggcaccg
     1861 ccttggtggc cggcgttgcc gccaaatatg cctgggatcg cggcgtcgat ctggtcgaga
     1921 aggaactgaa ccgcggcaaa gctgccaacg gcgcttccga cgaggacatc ttgcgggacg
     1981 aactggcctg aatggcttcc aaggtgtacg accccgagca acgcaaacgc atgatcactg
     2041 ggcctcagtg gtgggctcgc tgcaagcaaa tgaacgtgct ggactccttc atcaactact
     2101 atgattccga gaagcacgcc gagaacgccg tgatttttct gcatggtaac gctgcctcca
     2161 gctacctgtg gaggcacgtc gtgcctcaca tcgagcccgt ggctagatgc atcatccctg
     2221 atctgatcgg aatgggtaag tccggcaaga gcgggaatgg ctcatatcgc ctcctggatc
     2281 actacaagta cctcaccgct tggttcgagc tgctgaacct tccaaagaaa atcatctttg
     2341 tgggccacga ctggggggct tgtctggcct ttcactactc ctacgagcac caagacaaga
     2401 tcaaggccat cgtccatgct gagagtgtcg tggacgtgat cgagtcctgg gacgagtggc
     2461 ctgacatcga ggaggatatc gccctgatca agagcgaaga gggcgagaaa atggtgcttg
     2521 agaataactt cttcgtcgag accatgctcc caagcaagat catgcggaaa ctggagcctg
     2581 aggagttcgc tgcctacctg gagccattca aggagaaggg cgaggttaga cggcctaccc
     2641 tctcctggcc tcgcgagatc cctctcgtta agggaggcaa gcccgacgtc gtccagattg
     2701 tccgcaacta caacgcctac cttcgggcca gcgacgatct gcctaagatg ttcatcgagt
     2761 ccgaccctgg gttcttttcc aacgctattg tcgagggagc taagaagttc cctaacaccg
     2821 agttcgtgaa ggtgaagggc ctccacttca gccaggagga cgctccagat gaaatgggta
     2881 agtacatcaa gagcttcgtg gagcgcgtgc tgaagaacga gcagtaa
//