BBa_K624003 1 BBa_K624003 rep gene 2011-09-29T11:00:00Z 2015-05-08T01:12:55Z yes Released HQ 2013 only the plasmid contain rep gene can be used in magnetic bacteria AMB-1 false false _796_ 0 6664 9 In stock false yes false SHANG-JUI TSAI, CHUN-YU LAI annotation2148116 1 rbs from pMGT range2148116 1 1 24 annotation2148115 1 rep (pMGT) range2148115 1 28 857 annotation2148127 1 start range2148127 1 25 27 BBa_K624030 1 BBa_K624030 pYMB+[13-R]+[CHAMP-Y] 2011-10-03T11:00:00Z 2015-05-08T01:12:55Z BBa_K624004 pYMB indicates pUC19 with ori+Pmsp1+rep. In this construct, 13-R and Y-CHAMP are ligated in the downstream of ori+Pmsp1+rep, aimed at serving as optogenetic wireless neurostimulator with OFF state (no BRET between EYFP and rLuc) as default mode. This design is made in case EYFP fusion on N-terminal of mms13 has negative effects on its folding. false false _796_ 0 9029 9 Not in stock false to fit biobrick assembly standards false Shang-Jui Tsai component2152414 1 BBa_K624012 component2152403 1 BBa_K624004 component2152411 1 BBa_K624007 component2152406 1 BBa_K624012 component2152419 1 BBa_K624025 annotation2152414 1 BBa_K624012 range2152414 1 2957 2987 annotation2152411 1 BBa_K624007 range2152411 1 1188 2948 annotation2152406 1 BBa_K624012 range2152406 1 1151 1181 annotation2152419 1 BBa_K624025 range2152419 1 2994 3773 annotation2152403 1 BBa_K624004 range2152403 1 1 1142 BBa_K624007 1 BBa_K624007 mms13-rLuc 2011-09-30T11:00:00Z 2015-05-08T01:12:55Z mms13 from genomic DNA of Magnetospirillum magneticum AMB-1 alternative design for optogenetic wireless neurostimulation preventing blockade of signaling peptide located on helix 1 of mms13 false false _796_ 0 9029 9 It's complicated false to fit biobrick assembly rules false Shang-Jui Tsai annotation2148470 1 rLuc range2148470 1 373 1758 annotation2148471 1 stop range2148471 1 1759 1761 annotation2148469 1 start range2148469 1 1 3 annotation2148468 1 mms13 range2148468 1 4 372 BBa_K624004 1 BBa_K624004 ori+Pmsp1+rep (pYMB essentials) 2011-09-29T11:00:00Z 2015-05-08T01:12:55Z Express in AMB-1 Released HQ 2013 express in AMB-1 false false _796_ 0 6664 9 In stock false Express in AMB-1 false SHANG-JUI TSAI, CHUN-YU LAI component2148123 1 BBa_K624003 component2148120 1 BBa_K624000 annotation2148120 1 BBa_K624000 range2148120 1 1 277 annotation2148123 1 BBa_K624003 range2148123 1 286 1142 BBa_K624025 1 BBa_K624025 CHAMP-EYFP 2011-10-03T11:00:00Z 2015-05-08T01:12:55Z synthetic CHAMP DNA, EYFP extracted from parts registry inhibitor CHAMP with BRET receptor protein EYFP fused on its C-terminal false false _796_ 0 9029 9 It's complicated false to fit biobrick assembly standards. false Shang-Jui Tsai annotation2151924 1 BBa_K624010 range2151924 1 1 57 annotation2151925 1 start range2151925 1 1 3 annotation2151927 1 BBa_E0030 range2151927 1 58 780 annotation2151926 1 CHAMP (anti-H1[mms13]) range2151926 1 4 57 BBa_K624012 1 BBa_K624012 RBS from Pmsp3 2011-10-01T11:00:00Z 2015-05-08T01:12:55Z synthetic DNA predicted RBS site from Pmsp3, the strongest promoter of Magnetospirillum magneticum AMB-1 false false _796_ 0 9029 9 Not in stock false to fit biobrick assembly rules false Shang-Jui Tsai annotation2148845 1 artificial start codon range2148845 1 29 31 annotation2148844 1 rbs (from Pmsp3) range2148844 1 1 28 BBa_K624000 1 BBa_K624000 ori+Pmsp1 2011-09-29T11:00:00Z 2015-05-08T01:12:55Z express Released HQ 2013 express false false _796_ 0 6664 9 In stock false express false SHANG-JUI TSAI, CHUN-YU LAI annotation2148113 1 Pmsp1 range2148113 1 81 274 annotation2148114 1 start range2148114 1 275 277 annotation2148112 1 ori (pMGT) range2148112 1 1 80 BBa_K624030_sequence 1 tgatccgccagcgccgaagccatcataactgcattgaataattcaaagtgcattgaataaggcagggtgcataaggcgcagcggtaacgccgcctcgccggtggaagggtgttgtgtggcatccatgccacagtgcggagttttcgatacagccgacgtcccgcctcgcttgactggcggcgcccatcatggcgtgatggcgaccgttcgttcatttggatagcggtcgcggccattgggggtcgttttcgcaaagccaaagagaggagataccatgtactagagcgggagagggagctgatcccgctgatggtgacgctgacaggccatcacaatgcacggatgagcctcgctgaatgtctgacggtgatgaagaaggccaagcagagccttcaccagtctggcccatggaagcgccatgttggcccggtggtcgaagggcacgtcacggcaacggaggtcaatcggtcacgccgccatggctggcacgtccattttcacgtcctgatgctgattgaggccgccagcgaggccgaagcgattgcgctcgtgacccgacacctcgaacctgaatggctccggcagcttgagcgccaaggcagctggggcggcgaacatgcgttagacgtccagggcgcagccgatgcggatcggtatgtggccaagtggggcgcggctgaggaattggctctgtcgggcgaaaagcgggcgcgcggcgacgccgaaattaagggtcaaacgccgtgggaattgttggccgctgcggctgatggtgacgtccaggctggccgcgattttgtggagtacgcgaccctgtttcatggcaaacgccagctcgtttggtcgcgaggactgaaagagcgtgtcggcctcaaggtggtggaagacgacgaggccgccggcgagctggaggatgtcgcagatagcgccatcaacgaagtggccgtgttgccgaggccgacttggcgtcacatcgtccggcgcaggctccgcgcggatctgctggccgccgctcgtgatgacggattcgatggcgtcgcgcggctgctcaatgagcatggcatcggcggagctatgcccggcggctggcgaccaatgcttagcgaatataccgagcatattgcgatggctgcgtgactctttactagaggtccaagccctatgagggagcctttctaatgtactagatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagatgccctttcaccttgccccctatctggcgaaatccgttcccggcgtcggcgttctcggcgccctggtcggcggcgccgccgccttggccaagaacgtccgcctcctgaaggaaaagcgcatcaccaataccgaagcggccatcgataccggcaaggaaaccgtcggcgccggcctggccaccgcgctttccgccgtggccgcgaccgccgtcggcggcggcctggttgtatcgctgggcaccgccttggtggccggcgttgccgccaaatatgcctgggatcgcggcgtcgatctggtcgagaaggaactgaaccgcggcaaagctgccaacggcgcttccgacgaggacatcttgcgggacgaactggccatggcttccaaggtgtacgaccccgagcaacgcaaacgcatgatcactgggcctcagtggtgggctcgctgcaagcaaatgaacgtgctggactccttcatcaactactatgattccgagaagcacgccgagaacgccgtgatttttctgcatggtaacgctgcctccagctacctgtggaggcacgtcgtgcctcacatcgagcccgtggctagatgcatcatccctgatctgatcggaatgggtaagtccggcaagagcgggaatggctcatatcgcctcctggatcactacaagtacctcaccgcttggttcgagctgctgaaccttccaaagaaaatcatctttgtgggccacgactggggggcttgtctggcctttcactactcctacgagcaccaagacaagatcaaggccatcgtccatgctgagagtgtcgtggacgtgatcgagtcctgggacgagtggcctgacatcgaggaggatatcgccctgatcaagagcgaagagggcgagaaaatggtgcttgagaataacttcttcgtcgagaccatgctcccaagcaagatcatgcggaaactggagcctgaggagttcgctgcctacctggagccattcaaggagaagggcgaggttagacggcctaccctctcctggcctcgcgagatccctctcgttaagggaggcaagcccgacgtcgtccagattgtccgcaactacaacgcctaccttcgggccagcgacgatctgcctaagatgttcatcgagtccgaccctgggttcttttccaacgctattgtcgagggagctaagaagttccctaacaccgagttcgtgaaggtgaagggcctccacttcagccaggaggacgctccagatgaaatgggtaagtacatcaagagcttcgtggagcgcgtgctgaagaacgagcagtaatactagaggtccaagccctatgagggagcctttctaatgtactagatgaagaagctgttcgtgctgctgatgttcatcatcgtggccctgctgtggaagaagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa BBa_K624007_sequence 1 atgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagatgccctttcaccttgccccctatctggcgaaatccgttcccggcgtcggcgttctcggcgccctggtcggcggcgccgccgccttggccaagaacgtccgcctcctgaaggaaaagcgcatcaccaataccgaagcggccatcgataccggcaaggaaaccgtcggcgccggcctggccaccgcgctttccgccgtggccgcgaccgccgtcggcggcggcctggttgtatcgctgggcaccgccttggtggccggcgttgccgccaaatatgcctgggatcgcggcgtcgatctggtcgagaaggaactgaaccgcggcaaagctgccaacggcgcttccgacgaggacatcttgcgggacgaactggccatggcttccaaggtgtacgaccccgagcaacgcaaacgcatgatcactgggcctcagtggtgggctcgctgcaagcaaatgaacgtgctggactccttcatcaactactatgattccgagaagcacgccgagaacgccgtgatttttctgcatggtaacgctgcctccagctacctgtggaggcacgtcgtgcctcacatcgagcccgtggctagatgcatcatccctgatctgatcggaatgggtaagtccggcaagagcgggaatggctcatatcgcctcctggatcactacaagtacctcaccgcttggttcgagctgctgaaccttccaaagaaaatcatctttgtgggccacgactggggggcttgtctggcctttcactactcctacgagcaccaagacaagatcaaggccatcgtccatgctgagagtgtcgtggacgtgatcgagtcctgggacgagtggcctgacatcgaggaggatatcgccctgatcaagagcgaagagggcgagaaaatggtgcttgagaataacttcttcgtcgagaccatgctcccaagcaagatcatgcggaaactggagcctgaggagttcgctgcctacctggagccattcaaggagaagggcgaggttagacggcctaccctctcctggcctcgcgagatccctctcgttaagggaggcaagcccgacgtcgtccagattgtccgcaactacaacgcctaccttcgggccagcgacgatctgcctaagatgttcatcgagtccgaccctgggttcttttccaacgctattgtcgagggagctaagaagttccctaacaccgagttcgtgaaggtgaagggcctccacttcagccaggaggacgctccagatgaaatgggtaagtacatcaagagcttcgtggagcgcgtgctgaagaacgagcagtaa BBa_K624000_sequence 1 tgatccgccagcgccgaagccatcataactgcattgaataattcaaagtgcattgaataaggcagggtgcataaggcgcagcggtaacgccgcctcgccggtggaagggtgttgtgtggcatccatgccacagtgcggagttttcgatacagccgacgtcccgcctcgcttgactggcggcgcccatcatggcgtgatggcgaccgttcgttcatttggatagcggtcgcggccattgggggtcgttttcgcaaagccaaagagaggagataccatg BBa_K624012_sequence 1 gtccaagccctatgagggagcctttctaatg BBa_K624025_sequence 1 atgaagaagctgttcgtgctgctgatgttcatcatcgtggccctgctgtggaagaagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa BBa_K624004_sequence 1 tgatccgccagcgccgaagccatcataactgcattgaataattcaaagtgcattgaataaggcagggtgcataaggcgcagcggtaacgccgcctcgccggtggaagggtgttgtgtggcatccatgccacagtgcggagttttcgatacagccgacgtcccgcctcgcttgactggcggcgcccatcatggcgtgatggcgaccgttcgttcatttggatagcggtcgcggccattgggggtcgttttcgcaaagccaaagagaggagataccatgtactagagcgggagagggagctgatcccgctgatggtgacgctgacaggccatcacaatgcacggatgagcctcgctgaatgtctgacggtgatgaagaaggccaagcagagccttcaccagtctggcccatggaagcgccatgttggcccggtggtcgaagggcacgtcacggcaacggaggtcaatcggtcacgccgccatggctggcacgtccattttcacgtcctgatgctgattgaggccgccagcgaggccgaagcgattgcgctcgtgacccgacacctcgaacctgaatggctccggcagcttgagcgccaaggcagctggggcggcgaacatgcgttagacgtccagggcgcagccgatgcggatcggtatgtggccaagtggggcgcggctgaggaattggctctgtcgggcgaaaagcgggcgcgcggcgacgccgaaattaagggtcaaacgccgtgggaattgttggccgctgcggctgatggtgacgtccaggctggccgcgattttgtggagtacgcgaccctgtttcatggcaaacgccagctcgtttggtcgcgaggactgaaagagcgtgtcggcctcaaggtggtggaagacgacgaggccgccggcgagctggaggatgtcgcagatagcgccatcaacgaagtggccgtgttgccgaggccgacttggcgtcacatcgtccggcgcaggctccgcgcggatctgctggccgccgctcgtgatgacggattcgatggcgtcgcgcggctgctcaatgagcatggcatcggcggagctatgcccggcggctggcgaccaatgcttagcgaatataccgagcatattgcgatggctgcgtgactctt BBa_K624003_sequence 1 cgggagagggagctgatcccgctgatggtgacgctgacaggccatcacaatgcacggatgagcctcgctgaatgtctgacggtgatgaagaaggccaagcagagccttcaccagtctggcccatggaagcgccatgttggcccggtggtcgaagggcacgtcacggcaacggaggtcaatcggtcacgccgccatggctggcacgtccattttcacgtcctgatgctgattgaggccgccagcgaggccgaagcgattgcgctcgtgacccgacacctcgaacctgaatggctccggcagcttgagcgccaaggcagctggggcggcgaacatgcgttagacgtccagggcgcagccgatgcggatcggtatgtggccaagtggggcgcggctgaggaattggctctgtcgggcgaaaagcgggcgcgcggcgacgccgaaattaagggtcaaacgccgtgggaattgttggccgctgcggctgatggtgacgtccaggctggccgcgattttgtggagtacgcgaccctgtttcatggcaaacgccagctcgtttggtcgcgaggactgaaagagcgtgtcggcctcaaggtggtggaagacgacgaggccgccggcgagctggaggatgtcgcagatagcgccatcaacgaagtggccgtgttgccgaggccgacttggcgtcacatcgtccggcgcaggctccgcgcggatctgctggccgccgctcgtgatgacggattcgatggcgtcgcgcggctgctcaatgagcatggcatcggcggagctatgcccggcggctggcgaccaatgcttagcgaatataccgagcatattgcgatggctgcgtgactctt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z