LOCUS       BBa_K624031             3317 bp    DNA     linear       25-Nov-2024
DEFINITION  pYMB essentials+[YN-13-R]+[CHAMP-YC]
ACCESSION   BBa_K624031
VERSION     BBa_K624031.1  
FEATURES             Location/Qualifiers
     misc_feature    1..80
                     /label=ori (pMGT)
     promoter        81..274
                     /label=Pmsp1
     misc_feature    275..277
                     /label=start
     promoter        1..277
                     /label=BBa_K624000
     CDS             313..1142
                     /label=rep (pMGT)
     RBS             286..309
                     /label=rbs from pMGT
     misc_feature    310..312
                     /label=start
     CDS             286..1142
                     /label=BBa_K624003
     CDS             1..1142
                     /label=BBa_K624004
     RBS             1151..1178
                     /label=rbs (from Pmsp3)
     misc_feature    1179..1181
                     /label=artificial start codon
     RBS             1151..1181
                     /label=BBa_K624012
     misc_feature    1188..1190
                     /label=start
     CDS             1191..1655
                     /label=YN (EYFP)
     CDS             1656..2030
                     /label=mms13
     CDS             2031..2963
                     /label=rLuc
     misc_feature    2964..2966
                     /label=stop
     CDS             1188..2966
                     /label=BBa_K624008
     RBS             2975..3002
                     /label=rbs (from Pmsp3)
     misc_feature    3003..3005
                     /label=artificial start codon
     RBS             2975..3005
                     /label=BBa_K624012
     misc_feature    3012..3068
                     /label=BBa_K624010
     misc_feature    3012..3014
                     /label=start
     CDS             3015..3068
                     /label=CHAMP (anti-H1[mms13])
     CDS             3069..3314
                     /label=YC (from EYFP)
     misc_feature    3315..3317
                     /label=stop
     CDS             3012..3317
                     /label=BBa_K624026
ORIGIN
        1 tgatccgcca gcgccgaagc catcataact gcattgaata attcaaagtg cattgaataa
       61 ggcagggtgc ataaggcgca gcggtaacgc cgcctcgccg gtggaagggt gttgtgtggc
      121 atccatgcca cagtgcggag ttttcgatac agccgacgtc ccgcctcgct tgactggcgg
      181 cgcccatcat ggcgtgatgg cgaccgttcg ttcatttgga tagcggtcgc ggccattggg
      241 ggtcgttttc gcaaagccaa agagaggaga taccatgtac tagagcggga gagggagctg
      301 atcccgctga tggtgacgct gacaggccat cacaatgcac ggatgagcct cgctgaatgt
      361 ctgacggtga tgaagaaggc caagcagagc cttcaccagt ctggcccatg gaagcgccat
      421 gttggcccgg tggtcgaagg gcacgtcacg gcaacggagg tcaatcggtc acgccgccat
      481 ggctggcacg tccattttca cgtcctgatg ctgattgagg ccgccagcga ggccgaagcg
      541 attgcgctcg tgacccgaca cctcgaacct gaatggctcc ggcagcttga gcgccaaggc
      601 agctggggcg gcgaacatgc gttagacgtc cagggcgcag ccgatgcgga tcggtatgtg
      661 gccaagtggg gcgcggctga ggaattggct ctgtcgggcg aaaagcgggc gcgcggcgac
      721 gccgaaatta agggtcaaac gccgtgggaa ttgttggccg ctgcggctga tggtgacgtc
      781 caggctggcc gcgattttgt ggagtacgcg accctgtttc atggcaaacg ccagctcgtt
      841 tggtcgcgag gactgaaaga gcgtgtcggc ctcaaggtgg tggaagacga cgaggccgcc
      901 ggcgagctgg aggatgtcgc agatagcgcc atcaacgaag tggccgtgtt gccgaggccg
      961 acttggcgtc acatcgtccg gcgcaggctc cgcgcggatc tgctggccgc cgctcgtgat
     1021 gacggattcg atggcgtcgc gcggctgctc aatgagcatg gcatcggcgg agctatgccc
     1081 ggcggctggc gaccaatgct tagcgaatat accgagcata ttgcgatggc tgcgtgactc
     1141 tttactagag gtccaagccc tatgagggag cctttctaat gtactagatg gtgtccaagg
     1201 gcgaggaact gttcaccggc gtggtgccca tcctggtgga gctggacggc gacgtgaacg
     1261 gccacaagtt cagcgtgtcc ggcgagggcg aaggcgacgc cacctacggc aagctgaccc
     1321 tgaagctgat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctg gtgaccaccc
     1381 tgtacctcca gtgcttcgcc agataccccg accacatgaa gcagcacgat ttcttcaaga
     1441 gcgccatgcc cgagggctac gtgcaggaac ggaccatctt cttcaaggac gacggcaact
     1501 acaagaccag agccgaagtg aagttcgagg gcgacacact ggtgaaccgg atcgagctga
     1561 agggcatcga cttcaaagag gacggcaata tcctgggcca caagctggaa tacaactaca
     1621 acagccacaa cgtgtacatc accgccgaca agcagatgcc ctttcacctt gccccctatc
     1681 tggcgaaatc cgttcccggc gtcggcgttc tcggcgccct ggtcggcggc gccgccgcct
     1741 tggccaagaa cgtccgcctc ctgaaggaaa agcgcatcac caataccgaa gcggccatcg
     1801 ataccggcaa ggaaaccgtc ggcgccggcc tggccaccgc gctttccgcc gtggccgcga
     1861 ccgccgtcgg cggcggcctg gttgtatcgc tgggcaccgc cttggtggcc ggcgttgccg
     1921 ccaaatatgc ctgggatcgc ggcgtcgatc tggtcgagaa ggaactgaac cgcggcaaag
     1981 ctgccaacgg cgcttccgac gaggacatct tgcgggacga actggcctga atggcttcca
     2041 aggtgtacga ccccgagcaa cgcaaacgca tgatcactgg gcctcagtgg tgggctcgct
     2101 gcaagcaaat gaacgtgctg gactccttca tcaactacta tgattccgag aagcacgccg
     2161 agaacgccgt gatttttctg catggtaacg ctgcctccag ctacctgtgg aggcacgtcg
     2221 tgcctcacat cgagcccgtg gctagatgca tcatccctga tctgatcgga atgggtaagt
     2281 ccggcaagag cgggaatggc tcatatcgcc tcctggatca ctacaagtac ctcaccgctt
     2341 ggttcgagct gctgaacctt ccaaagaaaa tcatctttgt gggccacgac tggggggctt
     2401 gtctggcctt tcactactcc tacgagcacc aagacaagat caaggccatc gtccatgctg
     2461 agagtgtcgt ggacgtgatc gagtcctggg acgagtggcc tgacatcgag gaggatatcg
     2521 ccctgatcaa gagcgaagag ggcgagaaaa tggtgcttga gaataacttc ttcgtcgaga
     2581 ccatgctccc aagcaagatc atgcggaaac tggagcctga ggagttcgct gcctacctgg
     2641 agccattcaa ggagaagggc gaggttagac ggcctaccct ctcctggcct cgcgagatcc
     2701 ctctcgttaa gggaggcaag cccgacgtcg tccagattgt ccgcaactac aacgcctacc
     2761 ttcgggccag cgacgatctg cctaagatgt tcatcgagtc cgaccctggg ttcttttcca
     2821 acgctattgt cgagggagct aagaagttcc ctaacaccga gttcgtgaag gtgaagggcc
     2881 tccacttcag ccaggaggac gctccagatg aaatgggtaa gtacatcaag agcttcgtgg
     2941 agcgcgtgct gaagaacgag cagtaatact agaggtccaa gccctatgag ggagcctttc
     3001 taatgtacta gatgaagaag ctgttcgtgc tgctgatgtt catcatcgtg gccctgctgt
     3061 ggaagaagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     3121 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     3181 tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc aacgagaagc
     3241 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     3301 agctgtacaa gtaataa
//