LOCUS BBa_K624031 3317 bp DNA linear 27-Dec-2024 DEFINITION pYMB essentials+[YN-13-R]+[CHAMP-YC] ACCESSION BBa_K624031 VERSION BBa_K624031.1 FEATURES Location/Qualifiers misc_feature 1..80 /label=ori (pMGT) promoter 81..274 /label=Pmsp1 misc_feature 275..277 /label=start promoter 1..277 /label=BBa_K624000 CDS 313..1142 /label=rep (pMGT) RBS 286..309 /label=rbs from pMGT misc_feature 310..312 /label=start CDS 286..1142 /label=BBa_K624003 CDS 1..1142 /label=BBa_K624004 RBS 1151..1178 /label=rbs (from Pmsp3) misc_feature 1179..1181 /label=artificial start codon RBS 1151..1181 /label=BBa_K624012 misc_feature 1188..1190 /label=start CDS 1191..1655 /label=YN (EYFP) CDS 1656..2030 /label=mms13 CDS 2031..2963 /label=rLuc misc_feature 2964..2966 /label=stop CDS 1188..2966 /label=BBa_K624008 RBS 2975..3002 /label=rbs (from Pmsp3) misc_feature 3003..3005 /label=artificial start codon RBS 2975..3005 /label=BBa_K624012 misc_feature 3012..3068 /label=BBa_K624010 misc_feature 3012..3014 /label=start CDS 3015..3068 /label=CHAMP (anti-H1[mms13]) CDS 3069..3314 /label=YC (from EYFP) misc_feature 3315..3317 /label=stop CDS 3012..3317 /label=BBa_K624026 ORIGIN 1 tgatccgcca gcgccgaagc catcataact gcattgaata attcaaagtg cattgaataa 61 ggcagggtgc ataaggcgca gcggtaacgc cgcctcgccg gtggaagggt gttgtgtggc 121 atccatgcca cagtgcggag ttttcgatac agccgacgtc ccgcctcgct tgactggcgg 181 cgcccatcat ggcgtgatgg cgaccgttcg ttcatttgga tagcggtcgc ggccattggg 241 ggtcgttttc gcaaagccaa agagaggaga taccatgtac tagagcggga gagggagctg 301 atcccgctga tggtgacgct gacaggccat cacaatgcac ggatgagcct cgctgaatgt 361 ctgacggtga tgaagaaggc caagcagagc cttcaccagt ctggcccatg gaagcgccat 421 gttggcccgg tggtcgaagg gcacgtcacg gcaacggagg tcaatcggtc acgccgccat 481 ggctggcacg tccattttca cgtcctgatg ctgattgagg ccgccagcga ggccgaagcg 541 attgcgctcg tgacccgaca cctcgaacct gaatggctcc ggcagcttga gcgccaaggc 601 agctggggcg gcgaacatgc gttagacgtc cagggcgcag ccgatgcgga tcggtatgtg 661 gccaagtggg gcgcggctga ggaattggct ctgtcgggcg aaaagcgggc gcgcggcgac 721 gccgaaatta agggtcaaac gccgtgggaa ttgttggccg ctgcggctga tggtgacgtc 781 caggctggcc gcgattttgt ggagtacgcg accctgtttc atggcaaacg ccagctcgtt 841 tggtcgcgag gactgaaaga gcgtgtcggc ctcaaggtgg tggaagacga cgaggccgcc 901 ggcgagctgg aggatgtcgc agatagcgcc atcaacgaag tggccgtgtt gccgaggccg 961 acttggcgtc acatcgtccg gcgcaggctc cgcgcggatc tgctggccgc cgctcgtgat 1021 gacggattcg atggcgtcgc gcggctgctc aatgagcatg gcatcggcgg agctatgccc 1081 ggcggctggc gaccaatgct tagcgaatat accgagcata ttgcgatggc tgcgtgactc 1141 tttactagag gtccaagccc tatgagggag cctttctaat gtactagatg gtgtccaagg 1201 gcgaggaact gttcaccggc gtggtgccca tcctggtgga gctggacggc gacgtgaacg 1261 gccacaagtt cagcgtgtcc ggcgagggcg aaggcgacgc cacctacggc aagctgaccc 1321 tgaagctgat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctg gtgaccaccc 1381 tgtacctcca gtgcttcgcc agataccccg accacatgaa gcagcacgat ttcttcaaga 1441 gcgccatgcc cgagggctac gtgcaggaac ggaccatctt cttcaaggac gacggcaact 1501 acaagaccag agccgaagtg aagttcgagg gcgacacact ggtgaaccgg atcgagctga 1561 agggcatcga cttcaaagag gacggcaata tcctgggcca caagctggaa tacaactaca 1621 acagccacaa cgtgtacatc accgccgaca agcagatgcc ctttcacctt gccccctatc 1681 tggcgaaatc cgttcccggc gtcggcgttc tcggcgccct ggtcggcggc gccgccgcct 1741 tggccaagaa cgtccgcctc ctgaaggaaa agcgcatcac caataccgaa gcggccatcg 1801 ataccggcaa ggaaaccgtc ggcgccggcc tggccaccgc gctttccgcc gtggccgcga 1861 ccgccgtcgg cggcggcctg gttgtatcgc tgggcaccgc cttggtggcc ggcgttgccg 1921 ccaaatatgc ctgggatcgc ggcgtcgatc tggtcgagaa ggaactgaac cgcggcaaag 1981 ctgccaacgg cgcttccgac gaggacatct tgcgggacga actggcctga atggcttcca 2041 aggtgtacga ccccgagcaa cgcaaacgca tgatcactgg gcctcagtgg tgggctcgct 2101 gcaagcaaat gaacgtgctg gactccttca tcaactacta tgattccgag aagcacgccg 2161 agaacgccgt gatttttctg catggtaacg ctgcctccag ctacctgtgg aggcacgtcg 2221 tgcctcacat cgagcccgtg gctagatgca tcatccctga tctgatcgga atgggtaagt 2281 ccggcaagag cgggaatggc tcatatcgcc tcctggatca ctacaagtac ctcaccgctt 2341 ggttcgagct gctgaacctt ccaaagaaaa tcatctttgt gggccacgac tggggggctt 2401 gtctggcctt tcactactcc tacgagcacc aagacaagat caaggccatc gtccatgctg 2461 agagtgtcgt ggacgtgatc gagtcctggg acgagtggcc tgacatcgag gaggatatcg 2521 ccctgatcaa gagcgaagag ggcgagaaaa tggtgcttga gaataacttc ttcgtcgaga 2581 ccatgctccc aagcaagatc atgcggaaac tggagcctga ggagttcgct gcctacctgg 2641 agccattcaa ggagaagggc gaggttagac ggcctaccct ctcctggcct cgcgagatcc 2701 ctctcgttaa gggaggcaag cccgacgtcg tccagattgt ccgcaactac aacgcctacc 2761 ttcgggccag cgacgatctg cctaagatgt tcatcgagtc cgaccctggg ttcttttcca 2821 acgctattgt cgagggagct aagaagttcc ctaacaccga gttcgtgaag gtgaagggcc 2881 tccacttcag ccaggaggac gctccagatg aaatgggtaa gtacatcaag agcttcgtgg 2941 agcgcgtgct gaagaacgag cagtaatact agaggtccaa gccctatgag ggagcctttc 3001 taatgtacta gatgaagaag ctgttcgtgc tgctgatgtt catcatcgtg gccctgctgt 3061 ggaagaagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca 3121 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc 3181 tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc aacgagaagc 3241 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg 3301 agctgtacaa gtaataa //