BBa_K624008 1 BBa_K624008 YN-mms13-rLuc 2011-09-30T11:00:00Z 2015-05-08T01:12:55Z mms13 from Magnetospirillum magneticum AMB-1 genomic DNA alternative design for optogenetic wireless neurostimulation aimed at testing bilaterally facilitated interaction between NY-NC and [helix 1 of mms13]-CHAMP false false _796_ 0 9029 9 It's complicated false to fit biobrick assembly rules false Shang-Jui Tsai annotation2148523 1 stop range2148523 1 1777 1779 annotation2148522 1 rLuc range2148522 1 844 1776 annotation2148519 1 start range2148519 1 1 3 annotation2148520 1 YN (EYFP) range2148520 1 4 468 annotation2148521 1 mms13 range2148521 1 469 843 BBa_K624031 1 BBa_K624031 pYMB essentials+[YN-13-R]+[CHAMP-YC] 2011-10-03T11:00:00Z 2015-05-08T01:12:55Z composite pYMB indicates pUC19 with ori+Pmsp1+rep. In this construct, YN-13-R and YC-CHAMP are ligated in the downstream of ori+Pmsp1+rep, aimed at serving as optogenetic wireless neurostimulator with OFF state (no BRET between EYFP and rLuc) as default mode. BiFC between YN and YC is anticipated in the default state as well. false false _796_ 0 9029 9 Not in stock true to fit biobrick assembly standards false Shang-Jui Tsai component2152445 1 BBa_K624026 component2152439 1 BBa_K624012 component2152427 1 BBa_K624004 component2152436 1 BBa_K624008 component2152430 1 BBa_K624012 annotation2152445 1 BBa_K624026 range2152445 1 3012 3317 annotation2152439 1 BBa_K624012 range2152439 1 2975 3005 annotation2152430 1 BBa_K624012 range2152430 1 1151 1181 annotation2152427 1 BBa_K624004 range2152427 1 1 1142 annotation2152436 1 BBa_K624008 range2152436 1 1188 2966 BBa_K624003 1 BBa_K624003 rep gene 2011-09-29T11:00:00Z 2015-05-08T01:12:55Z yes Released HQ 2013 only the plasmid contain rep gene can be used in magnetic bacteria AMB-1 false false _796_ 0 6664 9 In stock false yes false SHANG-JUI TSAI, CHUN-YU LAI annotation2148115 1 rep (pMGT) range2148115 1 28 857 annotation2148116 1 rbs from pMGT range2148116 1 1 24 annotation2148127 1 start range2148127 1 25 27 BBa_K624026 1 BBa_K624026 CHAMP-YC 2011-10-03T11:00:00Z 2015-05-08T01:12:55Z CHAMP as synthetic DNA, YC from parts registry inhibitor CHAMP with BRET receptor protein YC (C-fragment of EYFP) fused on its C-terminal false false _796_ 0 9029 9 It's complicated false to fit biobrick assembly standards false Shang-Jui Tsai annotation2151937 1 BBa_K624010 range2151937 1 1 57 annotation2151938 1 start range2151938 1 1 3 annotation2151941 1 stop range2151941 1 304 306 annotation2151940 1 YC (from EYFP) range2151940 1 58 303 annotation2151939 1 CHAMP (anti-H1[mms13]) range2151939 1 4 57 BBa_K624004 1 BBa_K624004 ori+Pmsp1+rep (pYMB essentials) 2011-09-29T11:00:00Z 2015-05-08T01:12:55Z Express in AMB-1 Released HQ 2013 express in AMB-1 false false _796_ 0 6664 9 In stock false Express in AMB-1 false SHANG-JUI TSAI, CHUN-YU LAI component2148123 1 BBa_K624003 component2148120 1 BBa_K624000 annotation2148120 1 BBa_K624000 range2148120 1 1 277 annotation2148123 1 BBa_K624003 range2148123 1 286 1142 BBa_K624012 1 BBa_K624012 RBS from Pmsp3 2011-10-01T11:00:00Z 2015-05-08T01:12:55Z synthetic DNA predicted RBS site from Pmsp3, the strongest promoter of Magnetospirillum magneticum AMB-1 false false _796_ 0 9029 9 Not in stock false to fit biobrick assembly rules false Shang-Jui Tsai annotation2148844 1 rbs (from Pmsp3) range2148844 1 1 28 annotation2148845 1 artificial start codon range2148845 1 29 31 BBa_K624000 1 BBa_K624000 ori+Pmsp1 2011-09-29T11:00:00Z 2015-05-08T01:12:55Z express Released HQ 2013 express false false _796_ 0 6664 9 In stock false express false SHANG-JUI TSAI, CHUN-YU LAI annotation2148112 1 ori (pMGT) range2148112 1 1 80 annotation2148114 1 start range2148114 1 275 277 annotation2148113 1 Pmsp1 range2148113 1 81 274 BBa_K624031_sequence 1 tgatccgccagcgccgaagccatcataactgcattgaataattcaaagtgcattgaataaggcagggtgcataaggcgcagcggtaacgccgcctcgccggtggaagggtgttgtgtggcatccatgccacagtgcggagttttcgatacagccgacgtcccgcctcgcttgactggcggcgcccatcatggcgtgatggcgaccgttcgttcatttggatagcggtcgcggccattgggggtcgttttcgcaaagccaaagagaggagataccatgtactagagcgggagagggagctgatcccgctgatggtgacgctgacaggccatcacaatgcacggatgagcctcgctgaatgtctgacggtgatgaagaaggccaagcagagccttcaccagtctggcccatggaagcgccatgttggcccggtggtcgaagggcacgtcacggcaacggaggtcaatcggtcacgccgccatggctggcacgtccattttcacgtcctgatgctgattgaggccgccagcgaggccgaagcgattgcgctcgtgacccgacacctcgaacctgaatggctccggcagcttgagcgccaaggcagctggggcggcgaacatgcgttagacgtccagggcgcagccgatgcggatcggtatgtggccaagtggggcgcggctgaggaattggctctgtcgggcgaaaagcgggcgcgcggcgacgccgaaattaagggtcaaacgccgtgggaattgttggccgctgcggctgatggtgacgtccaggctggccgcgattttgtggagtacgcgaccctgtttcatggcaaacgccagctcgtttggtcgcgaggactgaaagagcgtgtcggcctcaaggtggtggaagacgacgaggccgccggcgagctggaggatgtcgcagatagcgccatcaacgaagtggccgtgttgccgaggccgacttggcgtcacatcgtccggcgcaggctccgcgcggatctgctggccgccgctcgtgatgacggattcgatggcgtcgcgcggctgctcaatgagcatggcatcggcggagctatgcccggcggctggcgaccaatgcttagcgaatataccgagcatattgcgatggctgcgtgactctttactagaggtccaagccctatgagggagcctttctaatgtactagatggtgtccaagggcgaggaactgttcaccggcgtggtgcccatcctggtggagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgaaggcgacgccacctacggcaagctgaccctgaagctgatctgcaccaccggcaagctgcccgtgccctggcccaccctggtgaccaccctgtacctccagtgcttcgccagataccccgaccacatgaagcagcacgatttcttcaagagcgccatgcccgagggctacgtgcaggaacggaccatcttcttcaaggacgacggcaactacaagaccagagccgaagtgaagttcgagggcgacacactggtgaaccggatcgagctgaagggcatcgacttcaaagaggacggcaatatcctgggccacaagctggaatacaactacaacagccacaacgtgtacatcaccgccgacaagcagatgccctttcaccttgccccctatctggcgaaatccgttcccggcgtcggcgttctcggcgccctggtcggcggcgccgccgccttggccaagaacgtccgcctcctgaaggaaaagcgcatcaccaataccgaagcggccatcgataccggcaaggaaaccgtcggcgccggcctggccaccgcgctttccgccgtggccgcgaccgccgtcggcggcggcctggttgtatcgctgggcaccgccttggtggccggcgttgccgccaaatatgcctgggatcgcggcgtcgatctggtcgagaaggaactgaaccgcggcaaagctgccaacggcgcttccgacgaggacatcttgcgggacgaactggcctgaatggcttccaaggtgtacgaccccgagcaacgcaaacgcatgatcactgggcctcagtggtgggctcgctgcaagcaaatgaacgtgctggactccttcatcaactactatgattccgagaagcacgccgagaacgccgtgatttttctgcatggtaacgctgcctccagctacctgtggaggcacgtcgtgcctcacatcgagcccgtggctagatgcatcatccctgatctgatcggaatgggtaagtccggcaagagcgggaatggctcatatcgcctcctggatcactacaagtacctcaccgcttggttcgagctgctgaaccttccaaagaaaatcatctttgtgggccacgactggggggcttgtctggcctttcactactcctacgagcaccaagacaagatcaaggccatcgtccatgctgagagtgtcgtggacgtgatcgagtcctgggacgagtggcctgacatcgaggaggatatcgccctgatcaagagcgaagagggcgagaaaatggtgcttgagaataacttcttcgtcgagaccatgctcccaagcaagatcatgcggaaactggagcctgaggagttcgctgcctacctggagccattcaaggagaagggcgaggttagacggcctaccctctcctggcctcgcgagatccctctcgttaagggaggcaagcccgacgtcgtccagattgtccgcaactacaacgcctaccttcgggccagcgacgatctgcctaagatgttcatcgagtccgaccctgggttcttttccaacgctattgtcgagggagctaagaagttccctaacaccgagttcgtgaaggtgaagggcctccacttcagccaggaggacgctccagatgaaatgggtaagtacatcaagagcttcgtggagcgcgtgctgaagaacgagcagtaatactagaggtccaagccctatgagggagcctttctaatgtactagatgaagaagctgttcgtgctgctgatgttcatcatcgtggccctgctgtggaagaagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa BBa_K624000_sequence 1 tgatccgccagcgccgaagccatcataactgcattgaataattcaaagtgcattgaataaggcagggtgcataaggcgcagcggtaacgccgcctcgccggtggaagggtgttgtgtggcatccatgccacagtgcggagttttcgatacagccgacgtcccgcctcgcttgactggcggcgcccatcatggcgtgatggcgaccgttcgttcatttggatagcggtcgcggccattgggggtcgttttcgcaaagccaaagagaggagataccatg BBa_K624012_sequence 1 gtccaagccctatgagggagcctttctaatg BBa_K624008_sequence 1 atggtgtccaagggcgaggaactgttcaccggcgtggtgcccatcctggtggagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgaaggcgacgccacctacggcaagctgaccctgaagctgatctgcaccaccggcaagctgcccgtgccctggcccaccctggtgaccaccctgtacctccagtgcttcgccagataccccgaccacatgaagcagcacgatttcttcaagagcgccatgcccgagggctacgtgcaggaacggaccatcttcttcaaggacgacggcaactacaagaccagagccgaagtgaagttcgagggcgacacactggtgaaccggatcgagctgaagggcatcgacttcaaagaggacggcaatatcctgggccacaagctggaatacaactacaacagccacaacgtgtacatcaccgccgacaagcagatgccctttcaccttgccccctatctggcgaaatccgttcccggcgtcggcgttctcggcgccctggtcggcggcgccgccgccttggccaagaacgtccgcctcctgaaggaaaagcgcatcaccaataccgaagcggccatcgataccggcaaggaaaccgtcggcgccggcctggccaccgcgctttccgccgtggccgcgaccgccgtcggcggcggcctggttgtatcgctgggcaccgccttggtggccggcgttgccgccaaatatgcctgggatcgcggcgtcgatctggtcgagaaggaactgaaccgcggcaaagctgccaacggcgcttccgacgaggacatcttgcgggacgaactggcctgaatggcttccaaggtgtacgaccccgagcaacgcaaacgcatgatcactgggcctcagtggtgggctcgctgcaagcaaatgaacgtgctggactccttcatcaactactatgattccgagaagcacgccgagaacgccgtgatttttctgcatggtaacgctgcctccagctacctgtggaggcacgtcgtgcctcacatcgagcccgtggctagatgcatcatccctgatctgatcggaatgggtaagtccggcaagagcgggaatggctcatatcgcctcctggatcactacaagtacctcaccgcttggttcgagctgctgaaccttccaaagaaaatcatctttgtgggccacgactggggggcttgtctggcctttcactactcctacgagcaccaagacaagatcaaggccatcgtccatgctgagagtgtcgtggacgtgatcgagtcctgggacgagtggcctgacatcgaggaggatatcgccctgatcaagagcgaagagggcgagaaaatggtgcttgagaataacttcttcgtcgagaccatgctcccaagcaagatcatgcggaaactggagcctgaggagttcgctgcctacctggagccattcaaggagaagggcgaggttagacggcctaccctctcctggcctcgcgagatccctctcgttaagggaggcaagcccgacgtcgtccagattgtccgcaactacaacgcctaccttcgggccagcgacgatctgcctaagatgttcatcgagtccgaccctgggttcttttccaacgctattgtcgagggagctaagaagttccctaacaccgagttcgtgaaggtgaagggcctccacttcagccaggaggacgctccagatgaaatgggtaagtacatcaagagcttcgtggagcgcgtgctgaagaacgagcagtaa BBa_K624004_sequence 1 tgatccgccagcgccgaagccatcataactgcattgaataattcaaagtgcattgaataaggcagggtgcataaggcgcagcggtaacgccgcctcgccggtggaagggtgttgtgtggcatccatgccacagtgcggagttttcgatacagccgacgtcccgcctcgcttgactggcggcgcccatcatggcgtgatggcgaccgttcgttcatttggatagcggtcgcggccattgggggtcgttttcgcaaagccaaagagaggagataccatgtactagagcgggagagggagctgatcccgctgatggtgacgctgacaggccatcacaatgcacggatgagcctcgctgaatgtctgacggtgatgaagaaggccaagcagagccttcaccagtctggcccatggaagcgccatgttggcccggtggtcgaagggcacgtcacggcaacggaggtcaatcggtcacgccgccatggctggcacgtccattttcacgtcctgatgctgattgaggccgccagcgaggccgaagcgattgcgctcgtgacccgacacctcgaacctgaatggctccggcagcttgagcgccaaggcagctggggcggcgaacatgcgttagacgtccagggcgcagccgatgcggatcggtatgtggccaagtggggcgcggctgaggaattggctctgtcgggcgaaaagcgggcgcgcggcgacgccgaaattaagggtcaaacgccgtgggaattgttggccgctgcggctgatggtgacgtccaggctggccgcgattttgtggagtacgcgaccctgtttcatggcaaacgccagctcgtttggtcgcgaggactgaaagagcgtgtcggcctcaaggtggtggaagacgacgaggccgccggcgagctggaggatgtcgcagatagcgccatcaacgaagtggccgtgttgccgaggccgacttggcgtcacatcgtccggcgcaggctccgcgcggatctgctggccgccgctcgtgatgacggattcgatggcgtcgcgcggctgctcaatgagcatggcatcggcggagctatgcccggcggctggcgaccaatgcttagcgaatataccgagcatattgcgatggctgcgtgactctt BBa_K624003_sequence 1 cgggagagggagctgatcccgctgatggtgacgctgacaggccatcacaatgcacggatgagcctcgctgaatgtctgacggtgatgaagaaggccaagcagagccttcaccagtctggcccatggaagcgccatgttggcccggtggtcgaagggcacgtcacggcaacggaggtcaatcggtcacgccgccatggctggcacgtccattttcacgtcctgatgctgattgaggccgccagcgaggccgaagcgattgcgctcgtgacccgacacctcgaacctgaatggctccggcagcttgagcgccaaggcagctggggcggcgaacatgcgttagacgtccagggcgcagccgatgcggatcggtatgtggccaagtggggcgcggctgaggaattggctctgtcgggcgaaaagcgggcgcgcggcgacgccgaaattaagggtcaaacgccgtgggaattgttggccgctgcggctgatggtgacgtccaggctggccgcgattttgtggagtacgcgaccctgtttcatggcaaacgccagctcgtttggtcgcgaggactgaaagagcgtgtcggcctcaaggtggtggaagacgacgaggccgccggcgagctggaggatgtcgcagatagcgccatcaacgaagtggccgtgttgccgaggccgacttggcgtcacatcgtccggcgcaggctccgcgcggatctgctggccgccgctcgtgatgacggattcgatggcgtcgcgcggctgctcaatgagcatggcatcggcggagctatgcccggcggctggcgaccaatgcttagcgaatataccgagcatattgcgatggctgcgtgactctt BBa_K624026_sequence 1 atgaagaagctgttcgtgctgctgatgttcatcatcgtggccctgctgtggaagaagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z