LOCUS       BBa_K624041             1467 bp    DNA     linear       22-Dec-2024
DEFINITION  pT7-tetO+RBS+minC+mcherry
ACCESSION   BBa_K624041
VERSION     BBa_K624041.1  
FEATURES             Location/Qualifiers
     promoter        1..22
                     /label=BBa_K624038
     misc_feature    35..38
                     /label=conserved
     RBS             31..42
                     /label=BBa_B0034
     CDS             49..747
                     /label=minC
     primer_bind     727..747
                     /label=reverse minC primer binding
     primer_bind     49..76
                     /label=forward minC primer binding
     CDS             49..747
                     /label=BBa_K624033
     misc_feature    1..747
                     /label=BBa_K624040
     CDS             754..1464
                     /label=mCherry
     misc_feature    1105..1105
                     /label=C->T (removing PstI site)
     misc_feature    754..1467
                     /label=BBa_J06504
ORIGIN
        1 taatacgact cactatgata gatactagag aaagaggaga aatactagat gtcaaacacg
       61 ccaatcgagc ttaaaggcag tagcttcact ttatctgtgg ttcatctgca tgaggcagaa
      121 cctaaggtta tccatcaggc gctggaagac aaaatcgctc aggcccccgc atttttaaaa
      181 catgcccccg ttgtactcaa cgtcagtgca ctggaagacc cggtaaactg gtcagcgatg
      241 cataaggcgg tttcggcaac cggtttgcgg gttattggcg taagtggctg caaagatgcg
      301 caacttaaag ccgaaattga aaagatgggg ctgcctatcc tgacggaagg aaaggaaaaa
      361 gcgccacgtc cagctcccac accgcaggct ccagcgcaaa atacaacgcc ggtcacaaaa
      421 acgcgtttaa tagatacccc ggtgcgttcc ggtcagcgta tttatgctcc acaatgtgat
      481 ctgattgtta caagccacgt tagcgctggg gccgaattga ttgccgatgg gaacattcat
      541 gtctatggca tgatgcgcgg tcgtgcgctg gcaggggcaa gtggtgaccg ggaaacgcaa
      601 atattttgta cgaacctgat ggcggaactg gtgtccatcg caggtgaata ctggctgagt
      661 gatcaaatcc cagcagaatt ttatggcaaa gcggcgcgac tgcaattagt cgaaaacgct
      721 ttgaccgttc aaccgttaaa ttaataatac tagatggtga gcaagggcga ggaggataac
      781 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
      841 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
      901 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
      961 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
     1021 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
     1081 gtgaccgtga cccaggactc ctccttgcag gacggcgagt tcatctacaa ggtgaagctg
     1141 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     1201 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
     1261 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
     1321 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
     1381 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
     1441 ggcatggacg agctgtacaa gtaataa
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