LOCUS BBa_K624042 5086 bp DNA linear 26-Dec-2024 DEFINITION pT7 + RBS + LLO + RBS + Invasin + RBS + ECFP + Ter ACCESSION BBa_K624042 VERSION BBa_K624042.1 FEATURES Location/Qualifiers promoter 1..46 /label=BBa_I712074 RBS 55..67 /label=RBS-3\Weak RBS 61..64 /label=RBS misc_feature 55..67 /label=BBa_B0032 RBS 55..67 /label=BBa_B0032 CDS 74..1663 /label=protein CDS 74..1663 /label=BBa_K177026 RBS 1672..1684 /label=RBS-3\Weak RBS 1678..1681 /label=RBS misc_feature 1672..1684 /label=BBa_B0032 RBS 1672..1684 /label=BBa_B0032 CDS 1693..4518 /label=protein CDS 1693..4200 /label=BBa_K177010 misc_feature 4213..4216 /label=conserved RBS 4209..4220 /label=BBa_B0034 CDS 4227..4949 /label=BBa_E0020 stem_loop 4969..5012 /label=stem_loop misc_feature 4958..5037 /label=BBa_B0010 terminator 4958..5037 /label=BBa_B0010 stem_loop 5053..5072 /label=T7 TE misc_feature 5079..5079 /label=stop polyA_site 5073..5086 /label=polya misc_feature 5046..5086 /label=BBa_B0012 terminator 5046..5086 /label=BBa_B0012 misc_feature 4209..5086 /label=BBa_E0420 ORIGIN 1 taatacgact cactataggg aatacaagct acttgttctt tttgcatact agagtcacac 61 aggaaagtac tagatgaaaa aaataatgct agtttttatt acacttatat tagttagtct 121 accaattgcg caacaaactg aagcaaagga tgcatctgca ttcaataaag aaaatttaat 181 ttcatccata gcaccaccag catctccgcc tgcaagtcct aagacgccaa tcgaaaagaa 241 acacgcggat gaaatcgata agtatataca aggattggat tacaataaaa acaatgtatt 301 agtataccac ggagatgcag tgacaaatgt gccgccaaga aaaggttata aagatggaaa 361 tgaatatatc gttgtggaga aaaagaagaa atccatcaat caaaataatg cagatatcca 421 agttgtgaat gcaatttcga gcctaacata tccaggtgct ctcgtgaaag ccaattcgga 481 attagtagaa aatcaacccg atgttcttcc tgtcaaacgt gattcattaa cacttagcat 541 tgatttgcca ggaatgacta atcaagacaa taaaattgtt gtaaaaaatg ctactaaatc 601 gaacgttaac aacgcagtaa atacattagt ggaaagatgg aatgaaaaat atgctcaagc 661 ttatccaaat gtaagtgcaa aaattgatta tgatgacgaa atggcttaca gtgaatcaca 721 attaattgca aaatttggta cggcatttaa agctgtaaat aatagcttga atgtaaactt 781 cggcgcaatc agtgaaggga aaatgcaaga agaagtcatt agttttaaac aaatttacta 841 taacgtgaat gttaatgaac ctacaagacc ttccagattt ttcggcaaag ctgttactaa 901 agagcagttg caagcgcttg gagtgaatgc agaaaatcct cctgcatata tctcaagtgt 961 ggcatatggc cgtcaagttt atttgaaatt atcaactaat tcccatagta ccaaagtaaa 1021 agctgctttt gacgctgccg taagtgggaa atctgtctca ggtgatgtag aactgacaaa 1081 tatcatcaaa aattcttcct tcaaagccgt aatttacggt ggctccgcaa aagatgaagt 1141 tcaaatcatc gacggtaacc tcggagactt acgagatatt ttgaaaaaag gtgctacttt 1201 taaccgggaa acaccaggag ttcccattgc ctatacaaca aacttcttaa aagacaatga 1261 attagctgtt attaaaaaca actcagaata tattgaaaca acttcaaaag cttatacaga 1321 tggaaaaatc aacatcgatc actctggagg atacgttgct caattcaaca tctcttggga 1381 tgaaataaat tatgatcctg aaggtaacga aattgttcaa cataaaaact ggagcgaaaa 1441 caataaaagt aagctagctc atttcacatc gtccatctat ttgccaggta acgcaagaaa 1501 tattaatgtt tacgctaaag aatgcactgg tttagcttgg gaatggtgga gaacggtaat 1561 tgatgaccgg aacctaccgc ttgtgaaaaa tagaaatatc tccatctggg gcactacact 1621 ttatccgaaa tatagtaata gtgtagataa tccaatcgaa taatactaga gtcacacagg 1681 aaagtactag agctattgag gctccgcaca cagcggatac gccgtagccg cagatgttgg 1741 tatgtcgccg gtggtcatat ccatagtgac aaaatctgta ctggtctttt tagtccaata 1801 gttacccgat ggccactcag cgctatcata ggttgccaaa cttccccact ctccccacag 1861 agtcccgtct ggtgaacgcg aaccattact ggcgcgtgcg gactcgatca gcgcagtaaa 1921 atcagtgcgc tggcaatttc gactcgcctc gacacttgat tgcagtgaca tggtgccgga 1981 gaacacccac aaattaggtt tgaattgata tgttgccgta taactcggga attttttact 2041 ttttgccgtc acagtgacgg tgctaccata ggttttatag gcaatattga ctttaccctg 2101 attatcaacc ggcgcactgg ccgcatagga tgatgtccag tcatattgag tattattcgc 2161 cacatcgtca ttcatcacca attggaacgt ggctttatta aaggtagttt tcgggaagcc 2221 tttatctgtg gcaaattgct caccattcac cttaatgccg gttatcttcg gtactgggta 2281 cagggtgatt cttttctgca acaagtctag tgattccccc cccacctgcg gcgtaatatc 2341 gacatctcct acggaatttc ccaccacact ggcggtatag ttgtcagcat tttcggttac 2401 cgaactaata gttaccggaa caccactttg tataaattca agatctgtta tcccactgac 2461 aaactcatta tttttattac gcggaacaaa ggtgagtatc gactgcatgg agccatcggc 2521 aacaatatca gatggagaaa cgttaaaact tgagtttgtg acatctggcg gcagcacggc 2581 aaatcgtaca tcaacagtag cctgttgccc ctgaatatct aaagtgacga ctgacacccc 2641 aacagtgaag ctagttaatg caatgcttat cacaccctgt gcatcggttt tttttgtgac 2701 tttactggga agcgcaccat tactggtggt tatcaccccc tcttgctcgg caaccggcgt 2761 actatcaata ttagtcactg taaatttcac cgtgatggga gtcctgccat cagcaagcgc 2821 accatcatca gtcacttccg aggtaataac gaactgaggt tgctgcacaa taacggtcaa 2881 cgtagctgga ttagaactat tgccgtggtt atcgatagcc gtggcgctga gggtataggt 2941 attgaccgcc gcttcgctct cgccggtttt ccccacagta cgactcgctg gtgccatcgg 3001 tttataaggc ggtaagacca cattgtaatc tgttgcactg acgggtatta atttgccgcc 3061 ggcagcaacc cattgcgcat cattccagag aatacgttgt agtgcagatt gagactgtat 3121 ttgtgcacta acggaataaa cctgccccgg caggccggag agtacggcgg gtgaaaatgc 3181 caatttgata gtattctgtt tttggtattc cagaacaata tttggattgc gctcaaccag 3241 attataacgg ctctcagcca gtaaacgagt tccggccacc actgcggggc taaactgcga 3301 acgaaaactt tcgccgaggc gataatccat ttgtaagttc cattgtattt catgctgccg 3361 actttttccc attcgttgat cgacccccag tgtaatgaag gggatcggcg tataaataag 3421 ccctgtggtc accgcataag ggttactttg caggttatct ttaccaaata aagccacccg 3481 ctcaccacgg tactgctcat attttaattt cccgcccaat tgtggcagcg caggtaaata 3541 cgctttgacg tgaatgtcgc ccccgcttgc cgggcgctca ttatagtccg cgaaatcacg 3601 agattgatgc caaccattga ggcgaaaata accattggcc gataattgta aataatcagt 3661 ccaggcttct gcacccacgc caatacgatg attgtgcccg gtcatatcat tgtcatagaa 3721 agtgttaaag ccatacatcc aactttgttg gaaggtacgc accccagcgc cgatattaag 3781 ggtattgcga ctgtctttat ttcttatacc caactgacta aaaaagacat atgaagcaga 3841 gtcataccaa ggcaacagcc aatcaagaga actttctttg agggagaact ttttatcaaa 3901 gttaacattg acctgagttg tcccaaatct atttaaccag tgttttactt cctgatttgc 3961 cgcgtcattt accattgacc tggtgatatt ggatgcatta cccgataccg ccagactggt 4021 tccggtactg attatcttat tgaaagcctc attatgttca gcggggttct ctaacgcttc 4081 tgaattaaaa taatgttgct gtgagaaccc ataagtaaat attccaaata ttaaaccgat 4141 cgataatatt agcctgctaa ttattttagt cacagttagc gtattaaaaa atgaatacat 4201 tactagagaa agaggagaaa tactagatgg tgagcaaggg cgaggagctg ttcaccgggg 4261 tggtgcccat cctggtcgag ctggacggcg acgtgaacgg ccacaagttc agcgtgtccg 4321 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg 4381 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctggggc gtgcagtgct 4441 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag 4501 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg 4561 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca 4621 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacatcagc cacaacgtct 4681 atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc cgccacaaca 4741 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg 4801 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc 4861 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc 4921 tcggcatgga cgagctgtac aagtaataat actagagcca ggcatcaaat aaaacgaaag 4981 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac 5041 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata //