BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_E0420 1 BBa_E0420 ECFP (RBS+ LVA- TERM) (B0034.E0020.B0015) 2004-03-15T12:00:00Z 2015-08-31T04:07:26Z Released HQ 2013 Standard CFP output device w/o LVA tag. false true _1_ 0 24 7 In stock false true Caitlin Conboy component942797 1 BBa_B0012 component942780 1 BBa_B0034 component942787 1 BBa_B0010 component942782 1 BBa_E0020 annotation942797 1 BBa_B0012 range942797 1 838 878 annotation942780 1 BBa_B0034 range942780 1 1 12 annotation942787 1 BBa_B0010 range942787 1 750 829 annotation942782 1 BBa_E0020 range942782 1 19 741 BBa_K177026 1 BBa_K177026 listeriolysin O 399G->C - locus from Listeria monocytogenes 2009-07-13T11:00:00Z 2015-05-08T01:11:05Z Listeria monocytogenes Hemolytic protein - enables escape from endosome of a mammalian cell, following endocytosis. false false _284_ 0 4696 9 It's complicated true sequence after mutagenesis: 399G->C false Kamila Ornoch annotation2011266 1 protein range2011266 1 1 1590 BBa_K177010 1 BBa_K177010 invasine locus from yersinia 2009-07-06T11:00:00Z 2015-05-08T01:11:05Z Genomic DNA from Yersinia enable bacteria to enter a cell false false _284_ 0 4696 9 Not in stock false none false Kamila Ornoch annotation2010434 1 protein range2010434 1 1 2826 BBa_E0020 1 ecfp engineered cyan fluorescent protein derived from A. victoria GFP 2004-03-02T12:00:00Z 2015-08-31T04:07:25Z Released HQ 2013 -- No description -- false false _1_ 0 24 7 In stock false true Caitlin Conboy and Jennifer Braff BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_K624042 1 BBa_K624042 pT7 + RBS + LLO + RBS + Invasin + RBS + ECFP + Ter 2011-10-04T11:00:00Z 2015-05-08T01:12:55Z no. pT7 + RBS + LLO + RBS + Invasin + ECFP false false _796_ 0 8576 9 Not in stock false no. false Yi-Hong Liou component2155699 1 BBa_K177026 component2155704 1 BBa_K177010 component2155701 1 BBa_B0032 component2155714 1 BBa_E0420 component2155694 1 BBa_I712074 component2155696 1 BBa_B0032 annotation2155704 1 BBa_K177010 range2155704 1 1693 4200 annotation2155699 1 BBa_K177026 range2155699 1 74 1663 annotation2155701 1 BBa_B0032 range2155701 1 1672 1684 annotation2155694 1 BBa_I712074 range2155694 1 1 46 annotation2155714 1 BBa_E0420 range2155714 1 4209 5086 annotation2155696 1 BBa_B0032 range2155696 1 55 67 BBa_I712074 1 BBa_I712074 T7 promoter (strong promoter from T7 bacteriophage) 2007-10-21T11:00:00Z 2015-08-31T04:07:46Z T7 bacteriophage T7 promoter is very specific promoter which is transcribed only by specific T7 RNA polymerase. Usually this promoter is used in expression systems where T7 promoter is cotransfected with T7 RNA polymerase. That ensures strong transcription of desired genes. false false _130_ 0 1856 9 In stock false true Rok Gaber BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 BBa_B0032 1 BBa_B0032 RBS.3 (medium) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-2&quot; in figure 4-14 of thesis). <P> Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation7027 1 BBa_B0032 range7027 1 1 13 annotation1709 1 RBS-3\Weak range1709 1 1 13 annotation1710 1 RBS range1710 1 7 10 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_E0020_sequence 1 atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa BBa_K177026_sequence 1 atgaaaaaaataatgctagtttttattacacttatattagttagtctaccaattgcgcaacaaactgaagcaaaggatgcatctgcattcaataaagaaaatttaatttcatccatagcaccaccagcatctccgcctgcaagtcctaagacgccaatcgaaaagaaacacgcggatgaaatcgataagtatatacaaggattggattacaataaaaacaatgtattagtataccacggagatgcagtgacaaatgtgccgccaagaaaaggttataaagatggaaatgaatatatcgttgtggagaaaaagaagaaatccatcaatcaaaataatgcagatatccaagttgtgaatgcaatttcgagcctaacatatccaggtgctctcgtgaaagccaattcggaattagtagaaaatcaacccgatgttcttcctgtcaaacgtgattcattaacacttagcattgatttgccaggaatgactaatcaagacaataaaattgttgtaaaaaatgctactaaatcgaacgttaacaacgcagtaaatacattagtggaaagatggaatgaaaaatatgctcaagcttatccaaatgtaagtgcaaaaattgattatgatgacgaaatggcttacagtgaatcacaattaattgcaaaatttggtacggcatttaaagctgtaaataatagcttgaatgtaaacttcggcgcaatcagtgaagggaaaatgcaagaagaagtcattagttttaaacaaatttactataacgtgaatgttaatgaacctacaagaccttccagatttttcggcaaagctgttactaaagagcagttgcaagcgcttggagtgaatgcagaaaatcctcctgcatatatctcaagtgtggcatatggccgtcaagtttatttgaaattatcaactaattcccatagtaccaaagtaaaagctgcttttgacgctgccgtaagtgggaaatctgtctcaggtgatgtagaactgacaaatatcatcaaaaattcttccttcaaagccgtaatttacggtggctccgcaaaagatgaagttcaaatcatcgacggtaacctcggagacttacgagatattttgaaaaaaggtgctacttttaaccgggaaacaccaggagttcccattgcctatacaacaaacttcttaaaagacaatgaattagctgttattaaaaacaactcagaatatattgaaacaacttcaaaagcttatacagatggaaaaatcaacatcgatcactctggaggatacgttgctcaattcaacatctcttgggatgaaataaattatgatcctgaaggtaacgaaattgttcaacataaaaactggagcgaaaacaataaaagtaagctagctcatttcacatcgtccatctatttgccaggtaacgcaagaaatattaatgtttacgctaaagaatgcactggtttagcttgggaatggtggagaacggtaattgatgaccggaacctaccgcttgtgaaaaatagaaatatctccatctggggcactacactttatccgaaatatagtaatagtgtagataatccaatcgaataa BBa_B0034_sequence 1 aaagaggagaaa BBa_E0420_sequence 1 aaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0032_sequence 1 tcacacaggaaag BBa_I712074_sequence 1 taatacgactcactatagggaatacaagctacttgttctttttgca BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K177010_sequence 1 ctattgaggctccgcacacagcggatacgccgtagccgcagatgttggtatgtcgccggtggtcatatccatagtgacaaaatctgtactggtctttttagtccaatagttacccgatggccactcagcgctatcataggttgccaaacttccccactctccccacagagtcccgtctggtgaacgcgaaccattactggcgcgtgcggactcgatcagcgcagtaaaatcagtgcgctggcaatttcgactcgcctcgacacttgattgcagtgacatggtgccggagaacacccacaaattaggtttgaattgatatgttgccgtataactcgggaattttttactttttgccgtcacagtgacggtgctaccataggttttataggcaatattgactttaccctgattatcaaccggcgcactggccgcataggatgatgtccagtcatattgagtattattcgccacatcgtcattcatcaccaattggaacgtggctttattaaaggtagttttcgggaagcctttatctgtggcaaattgctcaccattcaccttaatgccggttatcttcggtactgggtacagggtgattcttttctgcaacaagtctagtgattccccccccacctgcggcgtaatatcgacatctcctacggaatttcccaccacactggcggtatagttgtcagcattttcggttaccgaactaatagttaccggaacaccactttgtataaattcaagatctgttatcccactgacaaactcattatttttattacgcggaacaaaggtgagtatcgactgcatggagccatcggcaacaatatcagatggagaaacgttaaaacttgagtttgtgacatctggcggcagcacggcaaatcgtacatcaacagtagcctgttgcccctgaatatctaaagtgacgactgacaccccaacagtgaagctagttaatgcaatgcttatcacaccctgtgcatcggttttttttgtgactttactgggaagcgcaccattactggtggttatcaccccctcttgctcggcaaccggcgtactatcaatattagtcactgtaaatttcaccgtgatgggagtcctgccatcagcaagcgcaccatcatcagtcacttccgaggtaataacgaactgaggttgctgcacaataacggtcaacgtagctggattagaactattgccgtggttatcgatagccgtggcgctgagggtataggtattgaccgccgcttcgctctcgccggttttccccacagtacgactcgctggtgccatcggtttataaggcggtaagaccacattgtaatctgttgcactgacgggtattaatttgccgccggcagcaacccattgcgcatcattccagagaatacgttgtagtgcagattgagactgtatttgtgcactaacggaataaacctgccccggcaggccggagagtacggcgggtgaaaatgccaatttgatagtattctgtttttggtattccagaacaatatttggattgcgctcaaccagattataacggctctcagccagtaaacgagttccggccaccactgcggggctaaactgcgaacgaaaactttcgccgaggcgataatccatttgtaagttccattgtatttcatgctgccgactttttcccattcgttgatcgacccccagtgtaatgaaggggatcggcgtataaataagccctgtggtcaccgcataagggttactttgcaggttatctttaccaaataaagccacccgctcaccacggtactgctcatattttaatttcccgcccaattgtggcagcgcaggtaaatacgctttgacgtgaatgtcgcccccgcttgccgggcgctcattatagtccgcgaaatcacgagattgatgccaaccattgaggcgaaaataaccattggccgataattgtaaataatcagtccaggcttctgcacccacgccaatacgatgattgtgcccggtcatatcattgtcatagaaagtgttaaagccatacatccaactttgttggaaggtacgcaccccagcgccgatattaagggtattgcgactgtctttatttcttatacccaactgactaaaaaagacatatgaagcagagtcataccaaggcaacagccaatcaagagaactttctttgagggagaactttttatcaaagttaacattgacctgagttgtcccaaatctatttaaccagtgttttacttcctgatttgccgcgtcatttaccattgacctggtgatattggatgcattacccgataccgccagactggttccggtactgattatcttattgaaagcctcattatgttcagcggggttctctaacgcttctgaattaaaataatgttgctgtgagaacccataagtaaatattccaaatattaaaccgatcgataatattagcctgctaattattttagtcacagttagcgtattaaaaaatgaatacat BBa_K624042_sequence 1 taatacgactcactatagggaatacaagctacttgttctttttgcatactagagtcacacaggaaagtactagatgaaaaaaataatgctagtttttattacacttatattagttagtctaccaattgcgcaacaaactgaagcaaaggatgcatctgcattcaataaagaaaatttaatttcatccatagcaccaccagcatctccgcctgcaagtcctaagacgccaatcgaaaagaaacacgcggatgaaatcgataagtatatacaaggattggattacaataaaaacaatgtattagtataccacggagatgcagtgacaaatgtgccgccaagaaaaggttataaagatggaaatgaatatatcgttgtggagaaaaagaagaaatccatcaatcaaaataatgcagatatccaagttgtgaatgcaatttcgagcctaacatatccaggtgctctcgtgaaagccaattcggaattagtagaaaatcaacccgatgttcttcctgtcaaacgtgattcattaacacttagcattgatttgccaggaatgactaatcaagacaataaaattgttgtaaaaaatgctactaaatcgaacgttaacaacgcagtaaatacattagtggaaagatggaatgaaaaatatgctcaagcttatccaaatgtaagtgcaaaaattgattatgatgacgaaatggcttacagtgaatcacaattaattgcaaaatttggtacggcatttaaagctgtaaataatagcttgaatgtaaacttcggcgcaatcagtgaagggaaaatgcaagaagaagtcattagttttaaacaaatttactataacgtgaatgttaatgaacctacaagaccttccagatttttcggcaaagctgttactaaagagcagttgcaagcgcttggagtgaatgcagaaaatcctcctgcatatatctcaagtgtggcatatggccgtcaagtttatttgaaattatcaactaattcccatagtaccaaagtaaaagctgcttttgacgctgccgtaagtgggaaatctgtctcaggtgatgtagaactgacaaatatcatcaaaaattcttccttcaaagccgtaatttacggtggctccgcaaaagatgaagttcaaatcatcgacggtaacctcggagacttacgagatattttgaaaaaaggtgctacttttaaccgggaaacaccaggagttcccattgcctatacaacaaacttcttaaaagacaatgaattagctgttattaaaaacaactcagaatatattgaaacaacttcaaaagcttatacagatggaaaaatcaacatcgatcactctggaggatacgttgctcaattcaacatctcttgggatgaaataaattatgatcctgaaggtaacgaaattgttcaacataaaaactggagcgaaaacaataaaagtaagctagctcatttcacatcgtccatctatttgccaggtaacgcaagaaatattaatgtttacgctaaagaatgcactggtttagcttgggaatggtggagaacggtaattgatgaccggaacctaccgcttgtgaaaaatagaaatatctccatctggggcactacactttatccgaaatatagtaatagtgtagataatccaatcgaataatactagagtcacacaggaaagtactagagctattgaggctccgcacacagcggatacgccgtagccgcagatgttggtatgtcgccggtggtcatatccatagtgacaaaatctgtactggtctttttagtccaatagttacccgatggccactcagcgctatcataggttgccaaacttccccactctccccacagagtcccgtctggtgaacgcgaaccattactggcgcgtgcggactcgatcagcgcagtaaaatcagtgcgctggcaatttcgactcgcctcgacacttgattgcagtgacatggtgccggagaacacccacaaattaggtttgaattgatatgttgccgtataactcgggaattttttactttttgccgtcacagtgacggtgctaccataggttttataggcaatattgactttaccctgattatcaaccggcgcactggccgcataggatgatgtccagtcatattgagtattattcgccacatcgtcattcatcaccaattggaacgtggctttattaaaggtagttttcgggaagcctttatctgtggcaaattgctcaccattcaccttaatgccggttatcttcggtactgggtacagggtgattcttttctgcaacaagtctagtgattccccccccacctgcggcgtaatatcgacatctcctacggaatttcccaccacactggcggtatagttgtcagcattttcggttaccgaactaatagttaccggaacaccactttgtataaattcaagatctgttatcccactgacaaactcattatttttattacgcggaacaaaggtgagtatcgactgcatggagccatcggcaacaatatcagatggagaaacgttaaaacttgagtttgtgacatctggcggcagcacggcaaatcgtacatcaacagtagcctgttgcccctgaatatctaaagtgacgactgacaccccaacagtgaagctagttaatgcaatgcttatcacaccctgtgcatcggttttttttgtgactttactgggaagcgcaccattactggtggttatcaccccctcttgctcggcaaccggcgtactatcaatattagtcactgtaaatttcaccgtgatgggagtcctgccatcagcaagcgcaccatcatcagtcacttccgaggtaataacgaactgaggttgctgcacaataacggtcaacgtagctggattagaactattgccgtggttatcgatagccgtggcgctgagggtataggtattgaccgccgcttcgctctcgccggttttccccacagtacgactcgctggtgccatcggtttataaggcggtaagaccacattgtaatctgttgcactgacgggtattaatttgccgccggcagcaacccattgcgcatcattccagagaatacgttgtagtgcagattgagactgtatttgtgcactaacggaataaacctgccccggcaggccggagagtacggcgggtgaaaatgccaatttgatagtattctgtttttggtattccagaacaatatttggattgcgctcaaccagattataacggctctcagccagtaaacgagttccggccaccactgcggggctaaactgcgaacgaaaactttcgccgaggcgataatccatttgtaagttccattgtatttcatgctgccgactttttcccattcgttgatcgacccccagtgtaatgaaggggatcggcgtataaataagccctgtggtcaccgcataagggttactttgcaggttatctttaccaaataaagccacccgctcaccacggtactgctcatattttaatttcccgcccaattgtggcagcgcaggtaaatacgctttgacgtgaatgtcgcccccgcttgccgggcgctcattatagtccgcgaaatcacgagattgatgccaaccattgaggcgaaaataaccattggccgataattgtaaataatcagtccaggcttctgcacccacgccaatacgatgattgtgcccggtcatatcattgtcatagaaagtgttaaagccatacatccaactttgttggaaggtacgcaccccagcgccgatattaagggtattgcgactgtctttatttcttatacccaactgactaaaaaagacatatgaagcagagtcataccaaggcaacagccaatcaagagaactttctttgagggagaactttttatcaaagttaacattgacctgagttgtcccaaatctatttaaccagtgttttacttcctgatttgccgcgtcatttaccattgacctggtgatattggatgcattacccgataccgccagactggttccggtactgattatcttattgaaagcctcattatgttcagcggggttctctaacgcttctgaattaaaataatgttgctgtgagaacccataagtaaatattccaaatattaaaccgatcgataatattagcctgctaattattttagtcacagttagcgtattaaaaaatgaatacattactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z