LOCUS BBa_K624043 5032 bp DNA linear 26-Dec-2024 DEFINITION RBS + LLO + RBS + Invasin + RBS + ECFP + Ter ACCESSION BBa_K624043 VERSION BBa_K624043.1 FEATURES Location/Qualifiers RBS 1..13 /label=RBS-3\Weak RBS 7..10 /label=RBS misc_feature 1..13 /label=BBa_B0032 RBS 1..13 /label=BBa_B0032 CDS 22..2847 /label=protein CDS 22..2529 /label=BBa_K177010 RBS 2538..2550 /label=RBS-3\Weak RBS 2544..2547 /label=RBS misc_feature 2538..2550 /label=BBa_B0032 RBS 2538..2550 /label=BBa_B0032 CDS 2557..4146 /label=protein CDS 2557..4146 /label=BBa_K177026 misc_feature 4159..4162 /label=conserved RBS 4155..4166 /label=BBa_B0034 CDS 4173..4895 /label=BBa_E0020 stem_loop 4915..4958 /label=stem_loop misc_feature 4904..4983 /label=BBa_B0010 terminator 4904..4983 /label=BBa_B0010 stem_loop 4999..5018 /label=T7 TE misc_feature 5025..5025 /label=stop polyA_site 5019..5032 /label=polya misc_feature 4992..5032 /label=BBa_B0012 terminator 4992..5032 /label=BBa_B0012 misc_feature 4155..5032 /label=BBa_E0420 ORIGIN 1 tcacacagga aagtactaga gctattgagg ctccgcacac agcggatacg ccgtagccgc 61 agatgttggt atgtcgccgg tggtcatatc catagtgaca aaatctgtac tggtcttttt 121 agtccaatag ttacccgatg gccactcagc gctatcatag gttgccaaac ttccccactc 181 tccccacaga gtcccgtctg gtgaacgcga accattactg gcgcgtgcgg actcgatcag 241 cgcagtaaaa tcagtgcgct ggcaatttcg actcgcctcg acacttgatt gcagtgacat 301 ggtgccggag aacacccaca aattaggttt gaattgatat gttgccgtat aactcgggaa 361 ttttttactt tttgccgtca cagtgacggt gctaccatag gttttatagg caatattgac 421 tttaccctga ttatcaaccg gcgcactggc cgcataggat gatgtccagt catattgagt 481 attattcgcc acatcgtcat tcatcaccaa ttggaacgtg gctttattaa aggtagtttt 541 cgggaagcct ttatctgtgg caaattgctc accattcacc ttaatgccgg ttatcttcgg 601 tactgggtac agggtgattc ttttctgcaa caagtctagt gattcccccc ccacctgcgg 661 cgtaatatcg acatctccta cggaatttcc caccacactg gcggtatagt tgtcagcatt 721 ttcggttacc gaactaatag ttaccggaac accactttgt ataaattcaa gatctgttat 781 cccactgaca aactcattat ttttattacg cggaacaaag gtgagtatcg actgcatgga 841 gccatcggca acaatatcag atggagaaac gttaaaactt gagtttgtga catctggcgg 901 cagcacggca aatcgtacat caacagtagc ctgttgcccc tgaatatcta aagtgacgac 961 tgacacccca acagtgaagc tagttaatgc aatgcttatc acaccctgtg catcggtttt 1021 ttttgtgact ttactgggaa gcgcaccatt actggtggtt atcaccccct cttgctcggc 1081 aaccggcgta ctatcaatat tagtcactgt aaatttcacc gtgatgggag tcctgccatc 1141 agcaagcgca ccatcatcag tcacttccga ggtaataacg aactgaggtt gctgcacaat 1201 aacggtcaac gtagctggat tagaactatt gccgtggtta tcgatagccg tggcgctgag 1261 ggtataggta ttgaccgccg cttcgctctc gccggttttc cccacagtac gactcgctgg 1321 tgccatcggt ttataaggcg gtaagaccac attgtaatct gttgcactga cgggtattaa 1381 tttgccgccg gcagcaaccc attgcgcatc attccagaga atacgttgta gtgcagattg 1441 agactgtatt tgtgcactaa cggaataaac ctgccccggc aggccggaga gtacggcggg 1501 tgaaaatgcc aatttgatag tattctgttt ttggtattcc agaacaatat ttggattgcg 1561 ctcaaccaga ttataacggc tctcagccag taaacgagtt ccggccacca ctgcggggct 1621 aaactgcgaa cgaaaacttt cgccgaggcg ataatccatt tgtaagttcc attgtatttc 1681 atgctgccga ctttttccca ttcgttgatc gacccccagt gtaatgaagg ggatcggcgt 1741 ataaataagc cctgtggtca ccgcataagg gttactttgc aggttatctt taccaaataa 1801 agccacccgc tcaccacggt actgctcata ttttaatttc ccgcccaatt gtggcagcgc 1861 aggtaaatac gctttgacgt gaatgtcgcc cccgcttgcc gggcgctcat tatagtccgc 1921 gaaatcacga gattgatgcc aaccattgag gcgaaaataa ccattggccg ataattgtaa 1981 ataatcagtc caggcttctg cacccacgcc aatacgatga ttgtgcccgg tcatatcatt 2041 gtcatagaaa gtgttaaagc catacatcca actttgttgg aaggtacgca ccccagcgcc 2101 gatattaagg gtattgcgac tgtctttatt tcttataccc aactgactaa aaaagacata 2161 tgaagcagag tcataccaag gcaacagcca atcaagagaa ctttctttga gggagaactt 2221 tttatcaaag ttaacattga cctgagttgt cccaaatcta tttaaccagt gttttacttc 2281 ctgatttgcc gcgtcattta ccattgacct ggtgatattg gatgcattac ccgataccgc 2341 cagactggtt ccggtactga ttatcttatt gaaagcctca ttatgttcag cggggttctc 2401 taacgcttct gaattaaaat aatgttgctg tgagaaccca taagtaaata ttccaaatat 2461 taaaccgatc gataatatta gcctgctaat tattttagtc acagttagcg tattaaaaaa 2521 tgaatacatt actagagtca cacaggaaag tactagatga aaaaaataat gctagttttt 2581 attacactta tattagttag tctaccaatt gcgcaacaaa ctgaagcaaa ggatgcatct 2641 gcattcaata aagaaaattt aatttcatcc atagcaccac cagcatctcc gcctgcaagt 2701 cctaagacgc caatcgaaaa gaaacacgcg gatgaaatcg ataagtatat acaaggattg 2761 gattacaata aaaacaatgt attagtatac cacggagatg cagtgacaaa tgtgccgcca 2821 agaaaaggtt ataaagatgg aaatgaatat atcgttgtgg agaaaaagaa gaaatccatc 2881 aatcaaaata atgcagatat ccaagttgtg aatgcaattt cgagcctaac atatccaggt 2941 gctctcgtga aagccaattc ggaattagta gaaaatcaac ccgatgttct tcctgtcaaa 3001 cgtgattcat taacacttag cattgatttg ccaggaatga ctaatcaaga caataaaatt 3061 gttgtaaaaa atgctactaa atcgaacgtt aacaacgcag taaatacatt agtggaaaga 3121 tggaatgaaa aatatgctca agcttatcca aatgtaagtg caaaaattga ttatgatgac 3181 gaaatggctt acagtgaatc acaattaatt gcaaaatttg gtacggcatt taaagctgta 3241 aataatagct tgaatgtaaa cttcggcgca atcagtgaag ggaaaatgca agaagaagtc 3301 attagtttta aacaaattta ctataacgtg aatgttaatg aacctacaag accttccaga 3361 tttttcggca aagctgttac taaagagcag ttgcaagcgc ttggagtgaa tgcagaaaat 3421 cctcctgcat atatctcaag tgtggcatat ggccgtcaag tttatttgaa attatcaact 3481 aattcccata gtaccaaagt aaaagctgct tttgacgctg ccgtaagtgg gaaatctgtc 3541 tcaggtgatg tagaactgac aaatatcatc aaaaattctt ccttcaaagc cgtaatttac 3601 ggtggctccg caaaagatga agttcaaatc atcgacggta acctcggaga cttacgagat 3661 attttgaaaa aaggtgctac ttttaaccgg gaaacaccag gagttcccat tgcctataca 3721 acaaacttct taaaagacaa tgaattagct gttattaaaa acaactcaga atatattgaa 3781 acaacttcaa aagcttatac agatggaaaa atcaacatcg atcactctgg aggatacgtt 3841 gctcaattca acatctcttg ggatgaaata aattatgatc ctgaaggtaa cgaaattgtt 3901 caacataaaa actggagcga aaacaataaa agtaagctag ctcatttcac atcgtccatc 3961 tatttgccag gtaacgcaag aaatattaat gtttacgcta aagaatgcac tggtttagct 4021 tgggaatggt ggagaacggt aattgatgac cggaacctac cgcttgtgaa aaatagaaat 4081 atctccatct ggggcactac actttatccg aaatatagta atagtgtaga taatccaatc 4141 gaataatact agagaaagag gagaaatact agatggtgag caagggcgag gagctgttca 4201 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt gaacggccac aagttcagcg 4261 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca 4321 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tggggcgtgc 4381 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc 4441 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc 4501 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg 4561 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac atcagccaca 4621 acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc 4681 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg 4741 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca 4801 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga 4861 tcactctcgg catggacgag ctgtacaagt aataatacta gagccaggca tcaaataaaa 4921 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct 4981 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta //