LOCUS BBa_K624044 6100 bp DNA linear 22-Dec-2024 DEFINITION pYMB essentials + RBS + LLO + RBS + Invasin + RBS + ECFP ACCESSION BBa_K624044 VERSION BBa_K624044.1 FEATURES Location/Qualifiers misc_feature 1..80 /label=ori (pMGT) promoter 81..274 /label=Pmsp1 misc_feature 275..277 /label=start promoter 1..277 /label=BBa_K624000 CDS 313..1142 /label=rep (pMGT) RBS 286..309 /label=rbs from pMGT misc_feature 310..312 /label=start CDS 286..1142 /label=BBa_K624003 CDS 1..1142 /label=BBa_K624004 RBS 1151..1178 /label=rbs (from Pmsp3) misc_feature 1179..1181 /label=artificial start codon RBS 1151..1181 /label=BBa_K624012 CDS 1188..2777 /label=protein CDS 1188..2777 /label=BBa_K177026 RBS 2786..2813 /label=rbs (from Pmsp3) misc_feature 2814..2816 /label=artificial start codon RBS 2786..2816 /label=BBa_K624012 CDS 2825..5650 /label=protein CDS 2825..5332 /label=BBa_K177010 RBS 5341..5368 /label=rbs (from Pmsp3) misc_feature 5369..5371 /label=artificial start codon RBS 5341..5371 /label=BBa_K624012 CDS 5378..6100 /label=BBa_E0020 misc_feature 1151..6100 /label=BBa_K624047 ORIGIN 1 tgatccgcca gcgccgaagc catcataact gcattgaata attcaaagtg cattgaataa 61 ggcagggtgc ataaggcgca gcggtaacgc cgcctcgccg gtggaagggt gttgtgtggc 121 atccatgcca cagtgcggag ttttcgatac agccgacgtc ccgcctcgct tgactggcgg 181 cgcccatcat ggcgtgatgg cgaccgttcg ttcatttgga tagcggtcgc ggccattggg 241 ggtcgttttc gcaaagccaa agagaggaga taccatgtac tagagcggga gagggagctg 301 atcccgctga tggtgacgct gacaggccat cacaatgcac ggatgagcct cgctgaatgt 361 ctgacggtga tgaagaaggc caagcagagc cttcaccagt ctggcccatg gaagcgccat 421 gttggcccgg tggtcgaagg gcacgtcacg gcaacggagg tcaatcggtc acgccgccat 481 ggctggcacg tccattttca cgtcctgatg ctgattgagg ccgccagcga ggccgaagcg 541 attgcgctcg tgacccgaca cctcgaacct gaatggctcc ggcagcttga gcgccaaggc 601 agctggggcg gcgaacatgc gttagacgtc cagggcgcag ccgatgcgga tcggtatgtg 661 gccaagtggg gcgcggctga ggaattggct ctgtcgggcg aaaagcgggc gcgcggcgac 721 gccgaaatta agggtcaaac gccgtgggaa ttgttggccg ctgcggctga tggtgacgtc 781 caggctggcc gcgattttgt ggagtacgcg accctgtttc atggcaaacg ccagctcgtt 841 tggtcgcgag gactgaaaga gcgtgtcggc ctcaaggtgg tggaagacga cgaggccgcc 901 ggcgagctgg aggatgtcgc agatagcgcc atcaacgaag tggccgtgtt gccgaggccg 961 acttggcgtc acatcgtccg gcgcaggctc cgcgcggatc tgctggccgc cgctcgtgat 1021 gacggattcg atggcgtcgc gcggctgctc aatgagcatg gcatcggcgg agctatgccc 1081 ggcggctggc gaccaatgct tagcgaatat accgagcata ttgcgatggc tgcgtgactc 1141 tttactagag gtccaagccc tatgagggag cctttctaat gtactagatg aaaaaaataa 1201 tgctagtttt tattacactt atattagtta gtctaccaat tgcgcaacaa actgaagcaa 1261 aggatgcatc tgcattcaat aaagaaaatt taatttcatc catagcacca ccagcatctc 1321 cgcctgcaag tcctaagacg ccaatcgaaa agaaacacgc ggatgaaatc gataagtata 1381 tacaaggatt ggattacaat aaaaacaatg tattagtata ccacggagat gcagtgacaa 1441 atgtgccgcc aagaaaaggt tataaagatg gaaatgaata tatcgttgtg gagaaaaaga 1501 agaaatccat caatcaaaat aatgcagata tccaagttgt gaatgcaatt tcgagcctaa 1561 catatccagg tgctctcgtg aaagccaatt cggaattagt agaaaatcaa cccgatgttc 1621 ttcctgtcaa acgtgattca ttaacactta gcattgattt gccaggaatg actaatcaag 1681 acaataaaat tgttgtaaaa aatgctacta aatcgaacgt taacaacgca gtaaatacat 1741 tagtggaaag atggaatgaa aaatatgctc aagcttatcc aaatgtaagt gcaaaaattg 1801 attatgatga cgaaatggct tacagtgaat cacaattaat tgcaaaattt ggtacggcat 1861 ttaaagctgt aaataatagc ttgaatgtaa acttcggcgc aatcagtgaa gggaaaatgc 1921 aagaagaagt cattagtttt aaacaaattt actataacgt gaatgttaat gaacctacaa 1981 gaccttccag atttttcggc aaagctgtta ctaaagagca gttgcaagcg cttggagtga 2041 atgcagaaaa tcctcctgca tatatctcaa gtgtggcata tggccgtcaa gtttatttga 2101 aattatcaac taattcccat agtaccaaag taaaagctgc ttttgacgct gccgtaagtg 2161 ggaaatctgt ctcaggtgat gtagaactga caaatatcat caaaaattct tccttcaaag 2221 ccgtaattta cggtggctcc gcaaaagatg aagttcaaat catcgacggt aacctcggag 2281 acttacgaga tattttgaaa aaaggtgcta cttttaaccg ggaaacacca ggagttccca 2341 ttgcctatac aacaaacttc ttaaaagaca atgaattagc tgttattaaa aacaactcag 2401 aatatattga aacaacttca aaagcttata cagatggaaa aatcaacatc gatcactctg 2461 gaggatacgt tgctcaattc aacatctctt gggatgaaat aaattatgat cctgaaggta 2521 acgaaattgt tcaacataaa aactggagcg aaaacaataa aagtaagcta gctcatttca 2581 catcgtccat ctatttgcca ggtaacgcaa gaaatattaa tgtttacgct aaagaatgca 2641 ctggtttagc ttgggaatgg tggagaacgg taattgatga ccggaaccta ccgcttgtga 2701 aaaatagaaa tatctccatc tggggcacta cactttatcc gaaatatagt aatagtgtag 2761 ataatccaat cgaataatac tagaggtcca agccctatga gggagccttt ctaatgtact 2821 agagctattg aggctccgca cacagcggat acgccgtagc cgcagatgtt ggtatgtcgc 2881 cggtggtcat atccatagtg acaaaatctg tactggtctt tttagtccaa tagttacccg 2941 atggccactc agcgctatca taggttgcca aacttcccca ctctccccac agagtcccgt 3001 ctggtgaacg cgaaccatta ctggcgcgtg cggactcgat cagcgcagta aaatcagtgc 3061 gctggcaatt tcgactcgcc tcgacacttg attgcagtga catggtgccg gagaacaccc 3121 acaaattagg tttgaattga tatgttgccg tataactcgg gaatttttta ctttttgccg 3181 tcacagtgac ggtgctacca taggttttat aggcaatatt gactttaccc tgattatcaa 3241 ccggcgcact ggccgcatag gatgatgtcc agtcatattg agtattattc gccacatcgt 3301 cattcatcac caattggaac gtggctttat taaaggtagt tttcgggaag cctttatctg 3361 tggcaaattg ctcaccattc accttaatgc cggttatctt cggtactggg tacagggtga 3421 ttcttttctg caacaagtct agtgattccc cccccacctg cggcgtaata tcgacatctc 3481 ctacggaatt tcccaccaca ctggcggtat agttgtcagc attttcggtt accgaactaa 3541 tagttaccgg aacaccactt tgtataaatt caagatctgt tatcccactg acaaactcat 3601 tatttttatt acgcggaaca aaggtgagta tcgactgcat ggagccatcg gcaacaatat 3661 cagatggaga aacgttaaaa cttgagtttg tgacatctgg cggcagcacg gcaaatcgta 3721 catcaacagt agcctgttgc ccctgaatat ctaaagtgac gactgacacc ccaacagtga 3781 agctagttaa tgcaatgctt atcacaccct gtgcatcggt tttttttgtg actttactgg 3841 gaagcgcacc attactggtg gttatcaccc cctcttgctc ggcaaccggc gtactatcaa 3901 tattagtcac tgtaaatttc accgtgatgg gagtcctgcc atcagcaagc gcaccatcat 3961 cagtcacttc cgaggtaata acgaactgag gttgctgcac aataacggtc aacgtagctg 4021 gattagaact attgccgtgg ttatcgatag ccgtggcgct gagggtatag gtattgaccg 4081 ccgcttcgct ctcgccggtt ttccccacag tacgactcgc tggtgccatc ggtttataag 4141 gcggtaagac cacattgtaa tctgttgcac tgacgggtat taatttgccg ccggcagcaa 4201 cccattgcgc atcattccag agaatacgtt gtagtgcaga ttgagactgt atttgtgcac 4261 taacggaata aacctgcccc ggcaggccgg agagtacggc gggtgaaaat gccaatttga 4321 tagtattctg tttttggtat tccagaacaa tatttggatt gcgctcaacc agattataac 4381 ggctctcagc cagtaaacga gttccggcca ccactgcggg gctaaactgc gaacgaaaac 4441 tttcgccgag gcgataatcc atttgtaagt tccattgtat ttcatgctgc cgactttttc 4501 ccattcgttg atcgaccccc agtgtaatga aggggatcgg cgtataaata agccctgtgg 4561 tcaccgcata agggttactt tgcaggttat ctttaccaaa taaagccacc cgctcaccac 4621 ggtactgctc atattttaat ttcccgccca attgtggcag cgcaggtaaa tacgctttga 4681 cgtgaatgtc gcccccgctt gccgggcgct cattatagtc cgcgaaatca cgagattgat 4741 gccaaccatt gaggcgaaaa taaccattgg ccgataattg taaataatca gtccaggctt 4801 ctgcacccac gccaatacga tgattgtgcc cggtcatatc attgtcatag aaagtgttaa 4861 agccatacat ccaactttgt tggaaggtac gcaccccagc gccgatatta agggtattgc 4921 gactgtcttt atttcttata cccaactgac taaaaaagac atatgaagca gagtcatacc 4981 aaggcaacag ccaatcaaga gaactttctt tgagggagaa ctttttatca aagttaacat 5041 tgacctgagt tgtcccaaat ctatttaacc agtgttttac ttcctgattt gccgcgtcat 5101 ttaccattga cctggtgata ttggatgcat tacccgatac cgccagactg gttccggtac 5161 tgattatctt attgaaagcc tcattatgtt cagcggggtt ctctaacgct tctgaattaa 5221 aataatgttg ctgtgagaac ccataagtaa atattccaaa tattaaaccg atcgataata 5281 ttagcctgct aattatttta gtcacagtta gcgtattaaa aaatgaatac attactagag 5341 gtccaagccc tatgagggag cctttctaat gtactagatg gtgagcaagg gcgaggagct 5401 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtgaacg gccacaagtt 5461 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat 5521 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctgggg 5581 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc 5641 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa 5701 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg 5761 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacatcag 5821 ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat 5881 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc 5941 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct 6001 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc 6061 cgggatcact ctcggcatgg acgagctgta caagtaataa //