##gff-version 3
##sequence-region BBa_K624045 1 1701
BBa_K624045	.	ribosome_entry_site	207	237	.	+	0	ID=BBa_K624012;Name=BBa_K624012
BBa_K624045	.	ribosome_entry_site	207	234	.	+	0	ID=annotation2148844;Name=rbs (from Pmsp3);Parent=BBa_K624012
BBa_K624045	.	start_codon	235	237	.	+	0	ID=annotation2148845;Name=artificial start codon;Parent=BBa_K624012
BBa_K624045	.	engineered_region	988	1701	.	+	0	ID=BBa_J06504;Name=BBa_J06504
BBa_K624045	.	sequence_alteration	1339	1339	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_K624045	.	CDS	988	1698	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_K624045	.	CDS	244	942	.	+	0	ID=BBa_K624033;Name=BBa_K624033
BBa_K624045	.	CDS	244	942	.	+	0	ID=annotation2154427;Name=minC;Parent=BBa_K624033
BBa_K624045	.	primer_binding_site	922	942	.	+	0	ID=annotation2154497;Name=reverse minC primer binding;Parent=BBa_K624033
BBa_K624045	.	primer_binding_site	244	271	.	+	0	ID=annotation2154503;Name=forward minC primer binding;Parent=BBa_K624033
BBa_K624045	.	promoter	1	198	.	+	0	ID=BBa_K624046;Name=BBa_K624046
BBa_K624045	.	non_covalent_binding_site	90	95	.	+	0	ID=annotation2155115;Name=-35 box;Parent=BBa_K624046
BBa_K624045	.	operator	96	110	.	+	0	ID=annotation2155116;Name=tetO;Parent=BBa_K624046
BBa_K624045	.	non_covalent_binding_site	111	119	.	+	0	ID=annotation2155117;Name=-10 box;Parent=BBa_K624046
BBa_K624045	.	promoter	1	198	.	+	0	ID=annotation2155114;Name=Pmsp1[tetO];Parent=BBa_K624046
BBa_K624045	.	ribosome_entry_site	951	981	.	+	0	ID=BBa_K624012;Name=BBa_K624012
BBa_K624045	.	ribosome_entry_site	951	978	.	+	0	ID=annotation2148844;Name=rbs (from Pmsp3);Parent=BBa_K624012
BBa_K624045	.	start_codon	979	981	.	+	0	ID=annotation2148845;Name=artificial start codon;Parent=BBa_K624012
>BBa_K624045
gcggtaacgccgcctcgccggtggaagggtgttgtgtggcatccatgccacagtgcggagttttcgatacagccgacgt
cccgcctcgcttgactcctatcagtgatagatggcgtgatggcgaccgttcgttcatttggatagcggtcgcggccatt
gggggtcgttttcgcaaagccaaagagaggagataccatgtactagaggtccaagccctatgagggagcctttctaatg
tactagatgtcaaacacgccaatcgagcttaaaggcagtagcttcactttatctgtggttcatctgcatgaggcagaac
ctaaggttatccatcaggcgctggaagacaaaatcgctcaggcccccgcatttttaaaacatgcccccgttgtactcaa
cgtcagtgcactggaagacccggtaaactggtcagcgatgcataaggcggtttcggcaaccggtttgcgggttattggc
gtaagtggctgcaaagatgcgcaacttaaagccgaaattgaaaagatggggctgcctatcctgacggaaggaaaggaaa
aagcgccacgtccagctcccacaccgcaggctccagcgcaaaatacaacgccggtcacaaaaacgcgtttaatagatac
cccggtgcgttccggtcagcgtatttatgctccacaatgtgatctgattgttacaagccacgttagcgctggggccgaa
ttgattgccgatgggaacattcatgtctatggcatgatgcgcggtcgtgcgctggcaggggcaagtggtgaccgggaaa
cgcaaatattttgtacgaacctgatggcggaactggtgtccatcgcaggtgaatactggctgagtgatcaaatcccagc
agaattttatggcaaagcggcgcgactgcaattagtcgaaaacgctttgaccgttcaaccgttaaattaataatactag
aggtccaagccctatgagggagcctttctaatgtactagatggtgagcaagggcgaggaggataacatggccatcatca
aggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgaggg
ccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctg
tcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttcc
ccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgca
ggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagacc
atgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagc
tgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgccta
caacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggc
cgccactccaccggcggcatggacgagctgtacaagtaataa