##gff-version 3
##sequence-region BBa_K624050 1 1477
BBa_K624050	.	engineered_region	764	1477	.	+	0	ID=BBa_J06504;Name=BBa_J06504
BBa_K624050	.	sequence_alteration	1115	1115	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_K624050	.	CDS	764	1474	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_K624050	.	ribosome_entry_site	736	757	.	+	0	ID=BBa_K624013;Name=BBa_K624013
BBa_K624050	.	ribosome_entry_site	736	757	.	+	0	ID=annotation2148846;Name=rbs (trunc.) from Pmsp3;Parent=BBa_K624013
BBa_K624050	.	ribosome_entry_site	1	22	.	+	0	ID=BBa_K624013;Name=BBa_K624013
BBa_K624050	.	ribosome_entry_site	1	22	.	+	0	ID=annotation2148846;Name=rbs (trunc.) from Pmsp3;Parent=BBa_K624013
BBa_K624050	.	CDS	29	727	.	+	0	ID=BBa_K624033;Name=BBa_K624033
BBa_K624050	.	CDS	29	727	.	+	0	ID=annotation2154427;Name=minC;Parent=BBa_K624033
BBa_K624050	.	primer_binding_site	707	727	.	+	0	ID=annotation2154497;Name=reverse minC primer binding;Parent=BBa_K624033
BBa_K624050	.	primer_binding_site	29	56	.	+	0	ID=annotation2154503;Name=forward minC primer binding;Parent=BBa_K624033
>BBa_K624050
gtccaagccctatgagggagcctactagatgtcaaacacgccaatcgagcttaaaggcagtagcttcactttatctgtg
gttcatctgcatgaggcagaacctaaggttatccatcaggcgctggaagacaaaatcgctcaggcccccgcatttttaa
aacatgcccccgttgtactcaacgtcagtgcactggaagacccggtaaactggtcagcgatgcataaggcggtttcggc
aaccggtttgcgggttattggcgtaagtggctgcaaagatgcgcaacttaaagccgaaattgaaaagatggggctgcct
atcctgacggaaggaaaggaaaaagcgccacgtccagctcccacaccgcaggctccagcgcaaaatacaacgccggtca
caaaaacgcgtttaatagataccccggtgcgttccggtcagcgtatttatgctccacaatgtgatctgattgttacaag
ccacgttagcgctggggccgaattgattgccgatgggaacattcatgtctatggcatgatgcgcggtcgtgcgctggca
ggggcaagtggtgaccgggaaacgcaaatattttgtacgaacctgatggcggaactggtgtccatcgcaggtgaatact
ggctgagtgatcaaatcccagcagaattttatggcaaagcggcgcgactgcaattagtcgaaaacgctttgaccgttca
accgttaaattaataatactagaggtccaagccctatgagggagcctactagatggtgagcaagggcgaggaggataac
atggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagg
gcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgc
ctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttg
aagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccagg
actcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaat
gcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaag
cagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagc
tgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacga
acgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataa