LOCUS BBa_K624051 6073 bp DNA linear 26-Nov-2024 DEFINITION pYMB essentials + RBS(trunc.) + LLO + RBS(trunc.) + Invasin + RBS(trunc.) + ECFP ACCESSION BBa_K624051 VERSION BBa_K624051.1 FEATURES Location/Qualifiers misc_feature 1..80 /label=ori (pMGT) promoter 81..274 /label=Pmsp1 misc_feature 275..277 /label=start promoter 1..277 /label=BBa_K624000 CDS 313..1142 /label=rep (pMGT) RBS 286..309 /label=rbs from pMGT misc_feature 310..312 /label=start CDS 286..1142 /label=BBa_K624003 CDS 1..1142 /label=BBa_K624004 RBS 1151..1172 /label=rbs (trunc.) from Pmsp3 RBS 1151..1172 /label=BBa_K624013 CDS 1179..2768 /label=protein CDS 1179..2768 /label=BBa_K177026 RBS 2777..2798 /label=rbs (trunc.) from Pmsp3 RBS 2777..2798 /label=BBa_K624013 CDS 2807..5632 /label=protein CDS 2807..5314 /label=BBa_K177010 RBS 5323..5344 /label=rbs (trunc.) from Pmsp3 RBS 5323..5344 /label=BBa_K624013 CDS 5351..6073 /label=BBa_E0020 misc_feature 1151..6073 /label=BBa_K624048 ORIGIN 1 tgatccgcca gcgccgaagc catcataact gcattgaata attcaaagtg cattgaataa 61 ggcagggtgc ataaggcgca gcggtaacgc cgcctcgccg gtggaagggt gttgtgtggc 121 atccatgcca cagtgcggag ttttcgatac agccgacgtc ccgcctcgct tgactggcgg 181 cgcccatcat ggcgtgatgg cgaccgttcg ttcatttgga tagcggtcgc ggccattggg 241 ggtcgttttc gcaaagccaa agagaggaga taccatgtac tagagcggga gagggagctg 301 atcccgctga tggtgacgct gacaggccat cacaatgcac ggatgagcct cgctgaatgt 361 ctgacggtga tgaagaaggc caagcagagc cttcaccagt ctggcccatg gaagcgccat 421 gttggcccgg tggtcgaagg gcacgtcacg gcaacggagg tcaatcggtc acgccgccat 481 ggctggcacg tccattttca cgtcctgatg ctgattgagg ccgccagcga ggccgaagcg 541 attgcgctcg tgacccgaca cctcgaacct gaatggctcc ggcagcttga gcgccaaggc 601 agctggggcg gcgaacatgc gttagacgtc cagggcgcag ccgatgcgga tcggtatgtg 661 gccaagtggg gcgcggctga ggaattggct ctgtcgggcg aaaagcgggc gcgcggcgac 721 gccgaaatta agggtcaaac gccgtgggaa ttgttggccg ctgcggctga tggtgacgtc 781 caggctggcc gcgattttgt ggagtacgcg accctgtttc atggcaaacg ccagctcgtt 841 tggtcgcgag gactgaaaga gcgtgtcggc ctcaaggtgg tggaagacga cgaggccgcc 901 ggcgagctgg aggatgtcgc agatagcgcc atcaacgaag tggccgtgtt gccgaggccg 961 acttggcgtc acatcgtccg gcgcaggctc cgcgcggatc tgctggccgc cgctcgtgat 1021 gacggattcg atggcgtcgc gcggctgctc aatgagcatg gcatcggcgg agctatgccc 1081 ggcggctggc gaccaatgct tagcgaatat accgagcata ttgcgatggc tgcgtgactc 1141 tttactagag gtccaagccc tatgagggag cctactagat gaaaaaaata atgctagttt 1201 ttattacact tatattagtt agtctaccaa ttgcgcaaca aactgaagca aaggatgcat 1261 ctgcattcaa taaagaaaat ttaatttcat ccatagcacc accagcatct ccgcctgcaa 1321 gtcctaagac gccaatcgaa aagaaacacg cggatgaaat cgataagtat atacaaggat 1381 tggattacaa taaaaacaat gtattagtat accacggaga tgcagtgaca aatgtgccgc 1441 caagaaaagg ttataaagat ggaaatgaat atatcgttgt ggagaaaaag aagaaatcca 1501 tcaatcaaaa taatgcagat atccaagttg tgaatgcaat ttcgagccta acatatccag 1561 gtgctctcgt gaaagccaat tcggaattag tagaaaatca acccgatgtt cttcctgtca 1621 aacgtgattc attaacactt agcattgatt tgccaggaat gactaatcaa gacaataaaa 1681 ttgttgtaaa aaatgctact aaatcgaacg ttaacaacgc agtaaataca ttagtggaaa 1741 gatggaatga aaaatatgct caagcttatc caaatgtaag tgcaaaaatt gattatgatg 1801 acgaaatggc ttacagtgaa tcacaattaa ttgcaaaatt tggtacggca tttaaagctg 1861 taaataatag cttgaatgta aacttcggcg caatcagtga agggaaaatg caagaagaag 1921 tcattagttt taaacaaatt tactataacg tgaatgttaa tgaacctaca agaccttcca 1981 gatttttcgg caaagctgtt actaaagagc agttgcaagc gcttggagtg aatgcagaaa 2041 atcctcctgc atatatctca agtgtggcat atggccgtca agtttatttg aaattatcaa 2101 ctaattccca tagtaccaaa gtaaaagctg cttttgacgc tgccgtaagt gggaaatctg 2161 tctcaggtga tgtagaactg acaaatatca tcaaaaattc ttccttcaaa gccgtaattt 2221 acggtggctc cgcaaaagat gaagttcaaa tcatcgacgg taacctcgga gacttacgag 2281 atattttgaa aaaaggtgct acttttaacc gggaaacacc aggagttccc attgcctata 2341 caacaaactt cttaaaagac aatgaattag ctgttattaa aaacaactca gaatatattg 2401 aaacaacttc aaaagcttat acagatggaa aaatcaacat cgatcactct ggaggatacg 2461 ttgctcaatt caacatctct tgggatgaaa taaattatga tcctgaaggt aacgaaattg 2521 ttcaacataa aaactggagc gaaaacaata aaagtaagct agctcatttc acatcgtcca 2581 tctatttgcc aggtaacgca agaaatatta atgtttacgc taaagaatgc actggtttag 2641 cttgggaatg gtggagaacg gtaattgatg accggaacct accgcttgtg aaaaatagaa 2701 atatctccat ctggggcact acactttatc cgaaatatag taatagtgta gataatccaa 2761 tcgaataata ctagaggtcc aagccctatg agggagccta ctagagctat tgaggctccg 2821 cacacagcgg atacgccgta gccgcagatg ttggtatgtc gccggtggtc atatccatag 2881 tgacaaaatc tgtactggtc tttttagtcc aatagttacc cgatggccac tcagcgctat 2941 cataggttgc caaacttccc cactctcccc acagagtccc gtctggtgaa cgcgaaccat 3001 tactggcgcg tgcggactcg atcagcgcag taaaatcagt gcgctggcaa tttcgactcg 3061 cctcgacact tgattgcagt gacatggtgc cggagaacac ccacaaatta ggtttgaatt 3121 gatatgttgc cgtataactc gggaattttt tactttttgc cgtcacagtg acggtgctac 3181 cataggtttt ataggcaata ttgactttac cctgattatc aaccggcgca ctggccgcat 3241 aggatgatgt ccagtcatat tgagtattat tcgccacatc gtcattcatc accaattgga 3301 acgtggcttt attaaaggta gttttcggga agcctttatc tgtggcaaat tgctcaccat 3361 tcaccttaat gccggttatc ttcggtactg ggtacagggt gattcttttc tgcaacaagt 3421 ctagtgattc cccccccacc tgcggcgtaa tatcgacatc tcctacggaa tttcccacca 3481 cactggcggt atagttgtca gcattttcgg ttaccgaact aatagttacc ggaacaccac 3541 tttgtataaa ttcaagatct gttatcccac tgacaaactc attattttta ttacgcggaa 3601 caaaggtgag tatcgactgc atggagccat cggcaacaat atcagatgga gaaacgttaa 3661 aacttgagtt tgtgacatct ggcggcagca cggcaaatcg tacatcaaca gtagcctgtt 3721 gcccctgaat atctaaagtg acgactgaca ccccaacagt gaagctagtt aatgcaatgc 3781 ttatcacacc ctgtgcatcg gttttttttg tgactttact gggaagcgca ccattactgg 3841 tggttatcac cccctcttgc tcggcaaccg gcgtactatc aatattagtc actgtaaatt 3901 tcaccgtgat gggagtcctg ccatcagcaa gcgcaccatc atcagtcact tccgaggtaa 3961 taacgaactg aggttgctgc acaataacgg tcaacgtagc tggattagaa ctattgccgt 4021 ggttatcgat agccgtggcg ctgagggtat aggtattgac cgccgcttcg ctctcgccgg 4081 ttttccccac agtacgactc gctggtgcca tcggtttata aggcggtaag accacattgt 4141 aatctgttgc actgacgggt attaatttgc cgccggcagc aacccattgc gcatcattcc 4201 agagaatacg ttgtagtgca gattgagact gtatttgtgc actaacggaa taaacctgcc 4261 ccggcaggcc ggagagtacg gcgggtgaaa atgccaattt gatagtattc tgtttttggt 4321 attccagaac aatatttgga ttgcgctcaa ccagattata acggctctca gccagtaaac 4381 gagttccggc caccactgcg gggctaaact gcgaacgaaa actttcgccg aggcgataat 4441 ccatttgtaa gttccattgt atttcatgct gccgactttt tcccattcgt tgatcgaccc 4501 ccagtgtaat gaaggggatc ggcgtataaa taagccctgt ggtcaccgca taagggttac 4561 tttgcaggtt atctttacca aataaagcca cccgctcacc acggtactgc tcatatttta 4621 atttcccgcc caattgtggc agcgcaggta aatacgcttt gacgtgaatg tcgcccccgc 4681 ttgccgggcg ctcattatag tccgcgaaat cacgagattg atgccaacca ttgaggcgaa 4741 aataaccatt ggccgataat tgtaaataat cagtccaggc ttctgcaccc acgccaatac 4801 gatgattgtg cccggtcata tcattgtcat agaaagtgtt aaagccatac atccaacttt 4861 gttggaaggt acgcacccca gcgccgatat taagggtatt gcgactgtct ttatttctta 4921 tacccaactg actaaaaaag acatatgaag cagagtcata ccaaggcaac agccaatcaa 4981 gagaactttc tttgagggag aactttttat caaagttaac attgacctga gttgtcccaa 5041 atctatttaa ccagtgtttt acttcctgat ttgccgcgtc atttaccatt gacctggtga 5101 tattggatgc attacccgat accgccagac tggttccggt actgattatc ttattgaaag 5161 cctcattatg ttcagcgggg ttctctaacg cttctgaatt aaaataatgt tgctgtgaga 5221 acccataagt aaatattcca aatattaaac cgatcgataa tattagcctg ctaattattt 5281 tagtcacagt tagcgtatta aaaaatgaat acattactag aggtccaagc cctatgaggg 5341 agcctactag atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt 5401 cgagctggac ggcgacgtga acggccacaa gttcagcgtg tccggcgagg gcgagggcga 5461 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc 5521 ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag tgcttcagcc gctaccccga 5581 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg 5641 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg 5701 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat 5761 cctggggcac aagctggagt acaactacat cagccacaac gtctatatca ccgccgacaa 5821 gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcagcgt 5881 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc 5941 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga 6001 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct 6061 gtacaagtaa taa //