BBa_K624052 1 BBa_K624052 Pmsp1(tetO) + RBS(trunc.) + minC + RBS(trunc.) + mcherry 2011-10-04T11:00:00Z 2015-05-08T01:12:55Z no. pYMB essentials + RBS(Pmsp3, 6 pbs trunc.) + minC + mcherry false false _796_ 0 8576 9 Not in stock false no. false Yi-Hong Liou component2155422 1 BBa_K624046 component2155433 1 BBa_J06504 component2155428 1 BBa_K624033 component2155430 1 BBa_K624013 component2155424 1 BBa_K624013 annotation2155424 1 BBa_K624013 range2155424 1 207 228 annotation2155433 1 BBa_J06504 range2155433 1 970 1683 annotation2155430 1 BBa_K624013 range2155430 1 942 963 annotation2155422 1 BBa_K624046 range2155422 1 1 198 annotation2155428 1 BBa_K624033 range2155428 1 235 933 BBa_K624033 1 BBa_K624033 minC cell division inhibitor (revised) 2011-10-04T11:00:00Z 2015-05-08T01:12:55Z E.coli K12 This part, minC, is known as a cell division inhibitor. It was first submitted by 2010 Warsaw iGEM team.(http://partsregistry.org/wiki/index.php?title=Part:BBa_K299806) However, there was a PstI site in the middle of the sequence, which makes it hard to do biobrick assembly. We designed primers to remove the site with a synonymous substitution. false false _796_ 0 8576 9 Not in stock false no. false Yi-Hong Liou annotation2154427 1 minC range2154427 1 1 699 annotation2154497 1 reverse minC primer binding range2154497 1 679 699 annotation2154503 1 forward minC primer binding range2154503 1 1 28 BBa_J06504 1 mCherry monomeric RFP optimized for bacteria 2005-07-17T11:00:00Z 2016-01-25T01:05:28Z mCherry from Roger Y. Tsien's lab, altered to be BioBrick compatible Released HQ 2013 mRFP (DsRed) derived, altered to be a biobrick by removing a PstI site and adding in the ends. false false _20_ 4206 340 20 In stock false <p> Made a point mutation to eliminate a PstI site in the middle and then added BioBrick ends using PCR. </p> <p> Sequenced using primers that bind to the pSB1A2 plasmid on either side of the brick. </p> true ytwang annotation1585829 1 mCherry range1585829 1 1 711 annotation1585833 1 C->T (removing PstI site) range1585833 1 352 352 BBa_K624046 1 BBa_K624046 Pmsp1 (tetO) 2011-10-04T11:00:00Z 2015-05-08T01:12:55Z PCR-engineered PCR product from AMB-1 genomic DNA The second strongest promoter on ''Magnetospirillum magneticum'' AMB-1,Pmsp1 with tetO between -10 and -35 boxes. false false _796_ 0 9029 9 Not in stock false to fit biobrick assembly standards false Shang-Jui Tsai annotation2155116 1 tetO range2155116 1 96 110 annotation2155117 1 -10 box range2155117 1 111 119 annotation2155115 1 -35 box range2155115 1 90 95 annotation2155114 1 Pmsp1[tetO] range2155114 1 1 198 BBa_K624013 1 BBa_K624013 RBS (6 bps truncated) from Pmsp3 2011-10-01T11:00:00Z 2015-05-08T01:12:55Z synthetic DNA RBS from Pmsp3 (the strongest promoter on Magnetospirillum magenticum AMB-1) with the last 6 bps truncated to fit biobrick assembly standards (X-S scar will fill in this gap automatically). false false _796_ 0 9029 9 Not in stock false to fit biobrick assembly rules false Shang-Jui Tsai annotation2148846 1 rbs (trunc.) from Pmsp3 range2148846 1 1 22 BBa_K624052_sequence 1 gcggtaacgccgcctcgccggtggaagggtgttgtgtggcatccatgccacagtgcggagttttcgatacagccgacgtcccgcctcgcttgactcctatcagtgatagatggcgtgatggcgaccgttcgttcatttggatagcggtcgcggccattgggggtcgttttcgcaaagccaaagagaggagataccatgtactagaggtccaagccctatgagggagcctactagatgtcaaacacgccaatcgagcttaaaggcagtagcttcactttatctgtggttcatctgcatgaggcagaacctaaggttatccatcaggcgctggaagacaaaatcgctcaggcccccgcatttttaaaacatgcccccgttgtactcaacgtcagtgcactggaagacccggtaaactggtcagcgatgcataaggcggtttcggcaaccggtttgcgggttattggcgtaagtggctgcaaagatgcgcaacttaaagccgaaattgaaaagatggggctgcctatcctgacggaaggaaaggaaaaagcgccacgtccagctcccacaccgcaggctccagcgcaaaatacaacgccggtcacaaaaacgcgtttaatagataccccggtgcgttccggtcagcgtatttatgctccacaatgtgatctgattgttacaagccacgttagcgctggggccgaattgattgccgatgggaacattcatgtctatggcatgatgcgcggtcgtgcgctggcaggggcaagtggtgaccgggaaacgcaaatattttgtacgaacctgatggcggaactggtgtccatcgcaggtgaatactggctgagtgatcaaatcccagcagaattttatggcaaagcggcgcgactgcaattagtcgaaaacgctttgaccgttcaaccgttaaattaataatactagaggtccaagccctatgagggagcctactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataa BBa_J06504_sequence 1 atggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataa BBa_K624033_sequence 1 atgtcaaacacgccaatcgagcttaaaggcagtagcttcactttatctgtggttcatctgcatgaggcagaacctaaggttatccatcaggcgctggaagacaaaatcgctcaggcccccgcatttttaaaacatgcccccgttgtactcaacgtcagtgcactggaagacccggtaaactggtcagcgatgcataaggcggtttcggcaaccggtttgcgggttattggcgtaagtggctgcaaagatgcgcaacttaaagccgaaattgaaaagatggggctgcctatcctgacggaaggaaaggaaaaagcgccacgtccagctcccacaccgcaggctccagcgcaaaatacaacgccggtcacaaaaacgcgtttaatagataccccggtgcgttccggtcagcgtatttatgctccacaatgtgatctgattgttacaagccacgttagcgctggggccgaattgattgccgatgggaacattcatgtctatggcatgatgcgcggtcgtgcgctggcaggggcaagtggtgaccgggaaacgcaaatattttgtacgaacctgatggcggaactggtgtccatcgcaggtgaatactggctgagtgatcaaatcccagcagaattttatggcaaagcggcgcgactgcaattagtcgaaaacgctttgaccgttcaaccgttaaattaataa BBa_K624046_sequence 1 gcggtaacgccgcctcgccggtggaagggtgttgtgtggcatccatgccacagtgcggagttttcgatacagccgacgtcccgcctcgcttgactcctatcagtgatagatggcgtgatggcgaccgttcgttcatttggatagcggtcgcggccattgggggtcgttttcgcaaagccaaagagaggagataccatg BBa_K624013_sequence 1 gtccaagccctatgagggagcc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z