##gff-version 3
##sequence-region BBa_K624052 1 1683
BBa_K624052	.	ribosome_entry_site	207	228	.	+	0	ID=BBa_K624013;Name=BBa_K624013
BBa_K624052	.	ribosome_entry_site	207	228	.	+	0	ID=annotation2148846;Name=rbs (trunc.) from Pmsp3;Parent=BBa_K624013
BBa_K624052	.	engineered_region	970	1683	.	+	0	ID=BBa_J06504;Name=BBa_J06504
BBa_K624052	.	sequence_alteration	1321	1321	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_K624052	.	CDS	970	1680	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_K624052	.	promoter	1	198	.	+	0	ID=BBa_K624046;Name=BBa_K624046
BBa_K624052	.	non_covalent_binding_site	90	95	.	+	0	ID=annotation2155115;Name=-35 box;Parent=BBa_K624046
BBa_K624052	.	non_covalent_binding_site	111	119	.	+	0	ID=annotation2155117;Name=-10 box;Parent=BBa_K624046
BBa_K624052	.	promoter	1	198	.	+	0	ID=annotation2155114;Name=Pmsp1[tetO];Parent=BBa_K624046
BBa_K624052	.	operator	96	110	.	+	0	ID=annotation2155116;Name=tetO;Parent=BBa_K624046
BBa_K624052	.	CDS	235	933	.	+	0	ID=BBa_K624033;Name=BBa_K624033
BBa_K624052	.	CDS	235	933	.	+	0	ID=annotation2154427;Name=minC;Parent=BBa_K624033
BBa_K624052	.	primer_binding_site	235	262	.	+	0	ID=annotation2154503;Name=forward minC primer binding;Parent=BBa_K624033
BBa_K624052	.	primer_binding_site	913	933	.	+	0	ID=annotation2154497;Name=reverse minC primer binding;Parent=BBa_K624033
BBa_K624052	.	ribosome_entry_site	942	963	.	+	0	ID=BBa_K624013;Name=BBa_K624013
BBa_K624052	.	ribosome_entry_site	942	963	.	+	0	ID=annotation2148846;Name=rbs (trunc.) from Pmsp3;Parent=BBa_K624013
>BBa_K624052
gcggtaacgccgcctcgccggtggaagggtgttgtgtggcatccatgccacagtgcggagttttcgatacagccgacgt
cccgcctcgcttgactcctatcagtgatagatggcgtgatggcgaccgttcgttcatttggatagcggtcgcggccatt
gggggtcgttttcgcaaagccaaagagaggagataccatgtactagaggtccaagccctatgagggagcctactagatg
tcaaacacgccaatcgagcttaaaggcagtagcttcactttatctgtggttcatctgcatgaggcagaacctaaggtta
tccatcaggcgctggaagacaaaatcgctcaggcccccgcatttttaaaacatgcccccgttgtactcaacgtcagtgc
actggaagacccggtaaactggtcagcgatgcataaggcggtttcggcaaccggtttgcgggttattggcgtaagtggc
tgcaaagatgcgcaacttaaagccgaaattgaaaagatggggctgcctatcctgacggaaggaaaggaaaaagcgccac
gtccagctcccacaccgcaggctccagcgcaaaatacaacgccggtcacaaaaacgcgtttaatagataccccggtgcg
ttccggtcagcgtatttatgctccacaatgtgatctgattgttacaagccacgttagcgctggggccgaattgattgcc
gatgggaacattcatgtctatggcatgatgcgcggtcgtgcgctggcaggggcaagtggtgaccgggaaacgcaaatat
tttgtacgaacctgatggcggaactggtgtccatcgcaggtgaatactggctgagtgatcaaatcccagcagaatttta
tggcaaagcggcgcgactgcaattagtcgaaaacgctttgaccgttcaaccgttaaattaataatactagaggtccaag
ccctatgagggagcctactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttca
aggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcac
ccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtac
ggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtggg
agcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatcta
caaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcc
tccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccact
acgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagtt
ggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggc
atggacgagctgtacaagtaataa