LOCUS       BBa_K624055             2637 bp    DNA     linear       22-Nov-2024
DEFINITION  pYMB essentials + RBS(Pmsp3) + tetR + RBS(Pmsp3) + GFP
ACCESSION   BBa_K624055
VERSION     BBa_K624055.1  
FEATURES             Location/Qualifiers
     misc_feature    1..80
                     /label=ori (pMGT)
     promoter        81..274
                     /label=Pmsp1
     misc_feature    275..277
                     /label=start
     promoter        1..277
                     /label=BBa_K624000
     CDS             313..1142
                     /label=rep (pMGT)
     RBS             286..309
                     /label=rbs from pMGT
     misc_feature    310..312
                     /label=start
     CDS             286..1142
                     /label=BBa_K624003
     CDS             1..1142
                     /label=BBa_K624004
     RBS             1151..1178
                     /label=rbs (from Pmsp3)
     misc_feature    1179..1181
                     /label=artificial start codon
     RBS             1151..1181
                     /label=BBa_K624012
     promoter        1..1181
                     /label=BBa_K624028
     CDS             1191..1807
                     /label=tetR
     misc_feature    1808..1841
                     /label=SsrA
     misc_feature    1848..1872
                     /label=Help:Barcodes
     CDS             1188..1872
                     /label=BBa_C0040
     RBS             1881..1908
                     /label=rbs (from Pmsp3)
     misc_feature    1909..1911
                     /label=artificial start codon
     RBS             1881..1911
                     /label=BBa_K624012
     CDS             1918..2637
                     /label=GFP protein
     CDS             1918..2637
                     /label=BBa_E0040
ORIGIN
        1 tgatccgcca gcgccgaagc catcataact gcattgaata attcaaagtg cattgaataa
       61 ggcagggtgc ataaggcgca gcggtaacgc cgcctcgccg gtggaagggt gttgtgtggc
      121 atccatgcca cagtgcggag ttttcgatac agccgacgtc ccgcctcgct tgactggcgg
      181 cgcccatcat ggcgtgatgg cgaccgttcg ttcatttgga tagcggtcgc ggccattggg
      241 ggtcgttttc gcaaagccaa agagaggaga taccatgtac tagagcggga gagggagctg
      301 atcccgctga tggtgacgct gacaggccat cacaatgcac ggatgagcct cgctgaatgt
      361 ctgacggtga tgaagaaggc caagcagagc cttcaccagt ctggcccatg gaagcgccat
      421 gttggcccgg tggtcgaagg gcacgtcacg gcaacggagg tcaatcggtc acgccgccat
      481 ggctggcacg tccattttca cgtcctgatg ctgattgagg ccgccagcga ggccgaagcg
      541 attgcgctcg tgacccgaca cctcgaacct gaatggctcc ggcagcttga gcgccaaggc
      601 agctggggcg gcgaacatgc gttagacgtc cagggcgcag ccgatgcgga tcggtatgtg
      661 gccaagtggg gcgcggctga ggaattggct ctgtcgggcg aaaagcgggc gcgcggcgac
      721 gccgaaatta agggtcaaac gccgtgggaa ttgttggccg ctgcggctga tggtgacgtc
      781 caggctggcc gcgattttgt ggagtacgcg accctgtttc atggcaaacg ccagctcgtt
      841 tggtcgcgag gactgaaaga gcgtgtcggc ctcaaggtgg tggaagacga cgaggccgcc
      901 ggcgagctgg aggatgtcgc agatagcgcc atcaacgaag tggccgtgtt gccgaggccg
      961 acttggcgtc acatcgtccg gcgcaggctc cgcgcggatc tgctggccgc cgctcgtgat
     1021 gacggattcg atggcgtcgc gcggctgctc aatgagcatg gcatcggcgg agctatgccc
     1081 ggcggctggc gaccaatgct tagcgaatat accgagcata ttgcgatggc tgcgtgactc
     1141 tttactagag gtccaagccc tatgagggag cctttctaat gtactagatg tccagattag
     1201 ataaaagtaa agtgattaac agcgcattag agctgcttaa tgaggtcgga atcgaaggtt
     1261 taacaacccg taaactcgcc cagaagctag gtgtagagca gcctacattg tattggcatg
     1321 taaaaaataa gcgggctttg ctcgacgcct tagccattga gatgttagat aggcaccata
     1381 ctcacttttg ccctttagaa ggggaaagct ggcaagattt tttacgtaat aacgctaaaa
     1441 gttttagatg tgctttacta agtcatcgcg atggagcaaa agtacattta ggtacacggc
     1501 ctacagaaaa acagtatgaa actctcgaaa atcaattagc ctttttatgc caacaaggtt
     1561 tttcactaga gaatgcatta tatgcactca gcgctgtggg gcattttact ttaggttgcg
     1621 tattggaaga tcaagagcat caagtcgcta aagaagaaag ggaaacacct actactgata
     1681 gtatgccgcc attattacga caagctatcg aattatttga tcaccaaggt gcagagccag
     1741 ccttcttatt cggccttgaa ttgatcatat gcggattaga aaaacaactt aaatgtgaaa
     1801 gtgggtccgc tgcaaacgac gaaaactacg ctttagtagc ttaataacac tgatagtgct
     1861 agtgtagatc actactagag gtccaagccc tatgagggag cctttctaat gtactagatg
     1921 cgtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
     1981 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
     2041 cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc
     2101 actactttcg gttatggtgt tcaatgcttt gcgagatacc cagatcatat gaaacagcat
     2161 gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa
     2221 gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat
     2281 agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg
     2341 gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc
     2401 aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat
     2461 tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg
     2521 tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt
     2581 gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa ataataa
//