BBa_C0040 1 tetR tetracycline repressor from transposon Tn10 (+LVA) 2003-01-31T12:00:00Z 2015-08-31T04:07:23Z Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999) Released HQ 2013 Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.</P> false true _1_ 0 24 7 In stock false References (unparsed) here: <p>Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). </P> <p> Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 </p> <P> References (unparsed) here: <p>Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). </P> <p> Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 </p> <P>BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA. <P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman. annotation2213989 1 Help:Barcodes range2213989 1 661 685 annotation23329 1 tetR range23329 1 4 620 annotation23330 1 SsrA range23330 1 621 654 BBa_K624004 1 BBa_K624004 ori+Pmsp1+rep (pYMB essentials) 2011-09-29T11:00:00Z 2015-05-08T01:12:55Z Express in AMB-1 Released HQ 2013 express in AMB-1 false false _796_ 0 6664 9 In stock false Express in AMB-1 false SHANG-JUI TSAI, CHUN-YU LAI component2148120 1 BBa_K624000 component2148123 1 BBa_K624003 annotation2148123 1 BBa_K624003 range2148123 1 286 1142 annotation2148120 1 BBa_K624000 range2148120 1 1 277 BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_K624000 1 BBa_K624000 ori+Pmsp1 2011-09-29T11:00:00Z 2015-05-08T01:12:55Z express Released HQ 2013 express false false _796_ 0 6664 9 In stock false express false SHANG-JUI TSAI, CHUN-YU LAI annotation2148112 1 ori (pMGT) range2148112 1 1 80 annotation2148114 1 start range2148114 1 275 277 annotation2148113 1 Pmsp1 range2148113 1 81 274 BBa_K624003 1 BBa_K624003 rep gene 2011-09-29T11:00:00Z 2015-05-08T01:12:55Z yes Released HQ 2013 only the plasmid contain rep gene can be used in magnetic bacteria AMB-1 false false _796_ 0 6664 9 In stock false yes false SHANG-JUI TSAI, CHUN-YU LAI annotation2148115 1 rep (pMGT) range2148115 1 28 857 annotation2148127 1 start range2148127 1 25 27 annotation2148116 1 rbs from pMGT range2148116 1 1 24 BBa_K624012 1 BBa_K624012 RBS from Pmsp3 2011-10-01T11:00:00Z 2015-05-08T01:12:55Z synthetic DNA predicted RBS site from Pmsp3, the strongest promoter of Magnetospirillum magneticum AMB-1 false false _796_ 0 9029 9 Not in stock false to fit biobrick assembly rules false Shang-Jui Tsai annotation2148844 1 rbs (from Pmsp3) range2148844 1 1 28 annotation2148845 1 artificial start codon range2148845 1 29 31 BBa_K624028 1 BBa_K624028 pYMB+rbs (Pmsp3) (expression vector essentials on Magnetospirillum magneticum AMB-1) 2011-10-03T11:00:00Z 2015-05-08T01:12:55Z composite essentials of pYMB as an expression vector false false _796_ 0 9029 9 It's complicated false to fit biobrick assembly standards false Shang-Jui Tsai component2152344 1 BBa_K624012 component2152341 1 BBa_K624004 annotation2152344 1 BBa_K624012 range2152344 1 1151 1181 annotation2152341 1 BBa_K624004 range2152341 1 1 1142 BBa_K624055 1 BBa_K624055 pYMB essentials + RBS(Pmsp3) + tetR + RBS(Pmsp3) + GFP 2011-10-04T11:00:00Z 2015-05-08T01:12:55Z no. Pmsp1 + RBS + tetR + RBS + GFP false false _796_ 0 8576 9 Not in stock false no. false Yi-Hong Liou component2264306 1 BBa_C0040 component2264311 1 BBa_E0040 component2264302 1 BBa_K624028 component2264309 1 BBa_K624012 annotation2264311 1 BBa_E0040 range2264311 1 1918 2637 annotation2264302 1 BBa_K624028 range2264302 1 1 1181 annotation2264309 1 BBa_K624012 range2264309 1 1881 1911 annotation2264306 1 BBa_C0040 range2264306 1 1188 1872 BBa_K624028_sequence 1 tgatccgccagcgccgaagccatcataactgcattgaataattcaaagtgcattgaataaggcagggtgcataaggcgcagcggtaacgccgcctcgccggtggaagggtgttgtgtggcatccatgccacagtgcggagttttcgatacagccgacgtcccgcctcgcttgactggcggcgcccatcatggcgtgatggcgaccgttcgttcatttggatagcggtcgcggccattgggggtcgttttcgcaaagccaaagagaggagataccatgtactagagcgggagagggagctgatcccgctgatggtgacgctgacaggccatcacaatgcacggatgagcctcgctgaatgtctgacggtgatgaagaaggccaagcagagccttcaccagtctggcccatggaagcgccatgttggcccggtggtcgaagggcacgtcacggcaacggaggtcaatcggtcacgccgccatggctggcacgtccattttcacgtcctgatgctgattgaggccgccagcgaggccgaagcgattgcgctcgtgacccgacacctcgaacctgaatggctccggcagcttgagcgccaaggcagctggggcggcgaacatgcgttagacgtccagggcgcagccgatgcggatcggtatgtggccaagtggggcgcggctgaggaattggctctgtcgggcgaaaagcgggcgcgcggcgacgccgaaattaagggtcaaacgccgtgggaattgttggccgctgcggctgatggtgacgtccaggctggccgcgattttgtggagtacgcgaccctgtttcatggcaaacgccagctcgtttggtcgcgaggactgaaagagcgtgtcggcctcaaggtggtggaagacgacgaggccgccggcgagctggaggatgtcgcagatagcgccatcaacgaagtggccgtgttgccgaggccgacttggcgtcacatcgtccggcgcaggctccgcgcggatctgctggccgccgctcgtgatgacggattcgatggcgtcgcgcggctgctcaatgagcatggcatcggcggagctatgcccggcggctggcgaccaatgcttagcgaatataccgagcatattgcgatggctgcgtgactctttactagaggtccaagccctatgagggagcctttctaatg BBa_K624000_sequence 1 tgatccgccagcgccgaagccatcataactgcattgaataattcaaagtgcattgaataaggcagggtgcataaggcgcagcggtaacgccgcctcgccggtggaagggtgttgtgtggcatccatgccacagtgcggagttttcgatacagccgacgtcccgcctcgcttgactggcggcgcccatcatggcgtgatggcgaccgttcgttcatttggatagcggtcgcggccattgggggtcgttttcgcaaagccaaagagaggagataccatg BBa_K624012_sequence 1 gtccaagccctatgagggagcctttctaatg BBa_K624055_sequence 1 tgatccgccagcgccgaagccatcataactgcattgaataattcaaagtgcattgaataaggcagggtgcataaggcgcagcggtaacgccgcctcgccggtggaagggtgttgtgtggcatccatgccacagtgcggagttttcgatacagccgacgtcccgcctcgcttgactggcggcgcccatcatggcgtgatggcgaccgttcgttcatttggatagcggtcgcggccattgggggtcgttttcgcaaagccaaagagaggagataccatgtactagagcgggagagggagctgatcccgctgatggtgacgctgacaggccatcacaatgcacggatgagcctcgctgaatgtctgacggtgatgaagaaggccaagcagagccttcaccagtctggcccatggaagcgccatgttggcccggtggtcgaagggcacgtcacggcaacggaggtcaatcggtcacgccgccatggctggcacgtccattttcacgtcctgatgctgattgaggccgccagcgaggccgaagcgattgcgctcgtgacccgacacctcgaacctgaatggctccggcagcttgagcgccaaggcagctggggcggcgaacatgcgttagacgtccagggcgcagccgatgcggatcggtatgtggccaagtggggcgcggctgaggaattggctctgtcgggcgaaaagcgggcgcgcggcgacgccgaaattaagggtcaaacgccgtgggaattgttggccgctgcggctgatggtgacgtccaggctggccgcgattttgtggagtacgcgaccctgtttcatggcaaacgccagctcgtttggtcgcgaggactgaaagagcgtgtcggcctcaaggtggtggaagacgacgaggccgccggcgagctggaggatgtcgcagatagcgccatcaacgaagtggccgtgttgccgaggccgacttggcgtcacatcgtccggcgcaggctccgcgcggatctgctggccgccgctcgtgatgacggattcgatggcgtcgcgcggctgctcaatgagcatggcatcggcggagctatgcccggcggctggcgaccaatgcttagcgaatataccgagcatattgcgatggctgcgtgactctttactagaggtccaagccctatgagggagcctttctaatgtactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagaggtccaagccctatgagggagcctttctaatgtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_C0040_sequence 1 atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac BBa_K624004_sequence 1 tgatccgccagcgccgaagccatcataactgcattgaataattcaaagtgcattgaataaggcagggtgcataaggcgcagcggtaacgccgcctcgccggtggaagggtgttgtgtggcatccatgccacagtgcggagttttcgatacagccgacgtcccgcctcgcttgactggcggcgcccatcatggcgtgatggcgaccgttcgttcatttggatagcggtcgcggccattgggggtcgttttcgcaaagccaaagagaggagataccatgtactagagcgggagagggagctgatcccgctgatggtgacgctgacaggccatcacaatgcacggatgagcctcgctgaatgtctgacggtgatgaagaaggccaagcagagccttcaccagtctggcccatggaagcgccatgttggcccggtggtcgaagggcacgtcacggcaacggaggtcaatcggtcacgccgccatggctggcacgtccattttcacgtcctgatgctgattgaggccgccagcgaggccgaagcgattgcgctcgtgacccgacacctcgaacctgaatggctccggcagcttgagcgccaaggcagctggggcggcgaacatgcgttagacgtccagggcgcagccgatgcggatcggtatgtggccaagtggggcgcggctgaggaattggctctgtcgggcgaaaagcgggcgcgcggcgacgccgaaattaagggtcaaacgccgtgggaattgttggccgctgcggctgatggtgacgtccaggctggccgcgattttgtggagtacgcgaccctgtttcatggcaaacgccagctcgtttggtcgcgaggactgaaagagcgtgtcggcctcaaggtggtggaagacgacgaggccgccggcgagctggaggatgtcgcagatagcgccatcaacgaagtggccgtgttgccgaggccgacttggcgtcacatcgtccggcgcaggctccgcgcggatctgctggccgccgctcgtgatgacggattcgatggcgtcgcgcggctgctcaatgagcatggcatcggcggagctatgcccggcggctggcgaccaatgcttagcgaatataccgagcatattgcgatggctgcgtgactctt BBa_K624003_sequence 1 cgggagagggagctgatcccgctgatggtgacgctgacaggccatcacaatgcacggatgagcctcgctgaatgtctgacggtgatgaagaaggccaagcagagccttcaccagtctggcccatggaagcgccatgttggcccggtggtcgaagggcacgtcacggcaacggaggtcaatcggtcacgccgccatggctggcacgtccattttcacgtcctgatgctgattgaggccgccagcgaggccgaagcgattgcgctcgtgacccgacacctcgaacctgaatggctccggcagcttgagcgccaaggcagctggggcggcgaacatgcgttagacgtccagggcgcagccgatgcggatcggtatgtggccaagtggggcgcggctgaggaattggctctgtcgggcgaaaagcgggcgcgcggcgacgccgaaattaagggtcaaacgccgtgggaattgttggccgctgcggctgatggtgacgtccaggctggccgcgattttgtggagtacgcgaccctgtttcatggcaaacgccagctcgtttggtcgcgaggactgaaagagcgtgtcggcctcaaggtggtggaagacgacgaggccgccggcgagctggaggatgtcgcagatagcgccatcaacgaagtggccgtgttgccgaggccgacttggcgtcacatcgtccggcgcaggctccgcgcggatctgctggccgccgctcgtgatgacggattcgatggcgtcgcgcggctgctcaatgagcatggcatcggcggagctatgcccggcggctggcgaccaatgcttagcgaatataccgagcatattgcgatggctgcgtgactctt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z