LOCUS       BBa_K624056             1886 bp    DNA     linear       03-May-2025
DEFINITION  pYMB essentials + RBS(Pmsp3) + minC
ACCESSION   BBa_K624056
VERSION     BBa_K624056.1  
FEATURES             Location/Qualifiers
     misc_feature    1..80
                     /label=ori (pMGT)
     promoter        81..274
                     /label=Pmsp1
     misc_feature    275..277
                     /label=start
     promoter        1..277
                     /label=BBa_K624000
     CDS             313..1142
                     /label=rep (pMGT)
     RBS             286..309
                     /label=rbs from pMGT
     misc_feature    310..312
                     /label=start
     CDS             286..1142
                     /label=BBa_K624003
     CDS             1..1142
                     /label=BBa_K624004
     RBS             1151..1178
                     /label=rbs (from Pmsp3)
     misc_feature    1179..1181
                     /label=artificial start codon
     RBS             1151..1181
                     /label=BBa_K624012
     promoter        1..1181
                     /label=BBa_K624028
     CDS             1188..1886
                     /label=minC
     primer_bind     1866..1886
                     /label=reverse minC primer binding
     primer_bind     1188..1215
                     /label=forward minC primer binding
     CDS             1188..1886
                     /label=BBa_K624033
ORIGIN
        1 tgatccgcca gcgccgaagc catcataact gcattgaata attcaaagtg cattgaataa
       61 ggcagggtgc ataaggcgca gcggtaacgc cgcctcgccg gtggaagggt gttgtgtggc
      121 atccatgcca cagtgcggag ttttcgatac agccgacgtc ccgcctcgct tgactggcgg
      181 cgcccatcat ggcgtgatgg cgaccgttcg ttcatttgga tagcggtcgc ggccattggg
      241 ggtcgttttc gcaaagccaa agagaggaga taccatgtac tagagcggga gagggagctg
      301 atcccgctga tggtgacgct gacaggccat cacaatgcac ggatgagcct cgctgaatgt
      361 ctgacggtga tgaagaaggc caagcagagc cttcaccagt ctggcccatg gaagcgccat
      421 gttggcccgg tggtcgaagg gcacgtcacg gcaacggagg tcaatcggtc acgccgccat
      481 ggctggcacg tccattttca cgtcctgatg ctgattgagg ccgccagcga ggccgaagcg
      541 attgcgctcg tgacccgaca cctcgaacct gaatggctcc ggcagcttga gcgccaaggc
      601 agctggggcg gcgaacatgc gttagacgtc cagggcgcag ccgatgcgga tcggtatgtg
      661 gccaagtggg gcgcggctga ggaattggct ctgtcgggcg aaaagcgggc gcgcggcgac
      721 gccgaaatta agggtcaaac gccgtgggaa ttgttggccg ctgcggctga tggtgacgtc
      781 caggctggcc gcgattttgt ggagtacgcg accctgtttc atggcaaacg ccagctcgtt
      841 tggtcgcgag gactgaaaga gcgtgtcggc ctcaaggtgg tggaagacga cgaggccgcc
      901 ggcgagctgg aggatgtcgc agatagcgcc atcaacgaag tggccgtgtt gccgaggccg
      961 acttggcgtc acatcgtccg gcgcaggctc cgcgcggatc tgctggccgc cgctcgtgat
     1021 gacggattcg atggcgtcgc gcggctgctc aatgagcatg gcatcggcgg agctatgccc
     1081 ggcggctggc gaccaatgct tagcgaatat accgagcata ttgcgatggc tgcgtgactc
     1141 tttactagag gtccaagccc tatgagggag cctttctaat gtactagatg tcaaacacgc
     1201 caatcgagct taaaggcagt agcttcactt tatctgtggt tcatctgcat gaggcagaac
     1261 ctaaggttat ccatcaggcg ctggaagaca aaatcgctca ggcccccgca tttttaaaac
     1321 atgcccccgt tgtactcaac gtcagtgcac tggaagaccc ggtaaactgg tcagcgatgc
     1381 ataaggcggt ttcggcaacc ggtttgcggg ttattggcgt aagtggctgc aaagatgcgc
     1441 aacttaaagc cgaaattgaa aagatggggc tgcctatcct gacggaagga aaggaaaaag
     1501 cgccacgtcc agctcccaca ccgcaggctc cagcgcaaaa tacaacgccg gtcacaaaaa
     1561 cgcgtttaat agataccccg gtgcgttccg gtcagcgtat ttatgctcca caatgtgatc
     1621 tgattgttac aagccacgtt agcgctgggg ccgaattgat tgccgatggg aacattcatg
     1681 tctatggcat gatgcgcggt cgtgcgctgg caggggcaag tggtgaccgg gaaacgcaaa
     1741 tattttgtac gaacctgatg gcggaactgg tgtccatcgc aggtgaatac tggctgagtg
     1801 atcaaatccc agcagaattt tatggcaaag cggcgcgact gcaattagtc gaaaacgctt
     1861 tgaccgttca accgttaaat taataa
//