LOCUS       BBa_K624057             2777 bp    DNA     linear       30-Dec-2025
DEFINITION  pYMB essentials + RBS(Pmsp3) + LLO
ACCESSION   BBa_K624057
VERSION     BBa_K624057.1  
FEATURES             Location/Qualifiers
     misc_feature    1..80
                     /label=ori (pMGT)
     promoter        81..274
                     /label=Pmsp1
     misc_feature    275..277
                     /label=start
     promoter        1..277
                     /label=BBa_K624000
     CDS             313..1142
                     /label=rep (pMGT)
     RBS             286..309
                     /label=rbs from pMGT
     misc_feature    310..312
                     /label=start
     CDS             286..1142
                     /label=BBa_K624003
     CDS             1..1142
                     /label=BBa_K624004
     RBS             1151..1178
                     /label=rbs (from Pmsp3)
     misc_feature    1179..1181
                     /label=artificial start codon
     RBS             1151..1181
                     /label=BBa_K624012
     promoter        1..1181
                     /label=BBa_K624028
     CDS             1188..2777
                     /label=protein
     CDS             1188..2777
                     /label=BBa_K177026
ORIGIN
        1 tgatccgcca gcgccgaagc catcataact gcattgaata attcaaagtg cattgaataa
       61 ggcagggtgc ataaggcgca gcggtaacgc cgcctcgccg gtggaagggt gttgtgtggc
      121 atccatgcca cagtgcggag ttttcgatac agccgacgtc ccgcctcgct tgactggcgg
      181 cgcccatcat ggcgtgatgg cgaccgttcg ttcatttgga tagcggtcgc ggccattggg
      241 ggtcgttttc gcaaagccaa agagaggaga taccatgtac tagagcggga gagggagctg
      301 atcccgctga tggtgacgct gacaggccat cacaatgcac ggatgagcct cgctgaatgt
      361 ctgacggtga tgaagaaggc caagcagagc cttcaccagt ctggcccatg gaagcgccat
      421 gttggcccgg tggtcgaagg gcacgtcacg gcaacggagg tcaatcggtc acgccgccat
      481 ggctggcacg tccattttca cgtcctgatg ctgattgagg ccgccagcga ggccgaagcg
      541 attgcgctcg tgacccgaca cctcgaacct gaatggctcc ggcagcttga gcgccaaggc
      601 agctggggcg gcgaacatgc gttagacgtc cagggcgcag ccgatgcgga tcggtatgtg
      661 gccaagtggg gcgcggctga ggaattggct ctgtcgggcg aaaagcgggc gcgcggcgac
      721 gccgaaatta agggtcaaac gccgtgggaa ttgttggccg ctgcggctga tggtgacgtc
      781 caggctggcc gcgattttgt ggagtacgcg accctgtttc atggcaaacg ccagctcgtt
      841 tggtcgcgag gactgaaaga gcgtgtcggc ctcaaggtgg tggaagacga cgaggccgcc
      901 ggcgagctgg aggatgtcgc agatagcgcc atcaacgaag tggccgtgtt gccgaggccg
      961 acttggcgtc acatcgtccg gcgcaggctc cgcgcggatc tgctggccgc cgctcgtgat
     1021 gacggattcg atggcgtcgc gcggctgctc aatgagcatg gcatcggcgg agctatgccc
     1081 ggcggctggc gaccaatgct tagcgaatat accgagcata ttgcgatggc tgcgtgactc
     1141 tttactagag gtccaagccc tatgagggag cctttctaat gtactagatg aaaaaaataa
     1201 tgctagtttt tattacactt atattagtta gtctaccaat tgcgcaacaa actgaagcaa
     1261 aggatgcatc tgcattcaat aaagaaaatt taatttcatc catagcacca ccagcatctc
     1321 cgcctgcaag tcctaagacg ccaatcgaaa agaaacacgc ggatgaaatc gataagtata
     1381 tacaaggatt ggattacaat aaaaacaatg tattagtata ccacggagat gcagtgacaa
     1441 atgtgccgcc aagaaaaggt tataaagatg gaaatgaata tatcgttgtg gagaaaaaga
     1501 agaaatccat caatcaaaat aatgcagata tccaagttgt gaatgcaatt tcgagcctaa
     1561 catatccagg tgctctcgtg aaagccaatt cggaattagt agaaaatcaa cccgatgttc
     1621 ttcctgtcaa acgtgattca ttaacactta gcattgattt gccaggaatg actaatcaag
     1681 acaataaaat tgttgtaaaa aatgctacta aatcgaacgt taacaacgca gtaaatacat
     1741 tagtggaaag atggaatgaa aaatatgctc aagcttatcc aaatgtaagt gcaaaaattg
     1801 attatgatga cgaaatggct tacagtgaat cacaattaat tgcaaaattt ggtacggcat
     1861 ttaaagctgt aaataatagc ttgaatgtaa acttcggcgc aatcagtgaa gggaaaatgc
     1921 aagaagaagt cattagtttt aaacaaattt actataacgt gaatgttaat gaacctacaa
     1981 gaccttccag atttttcggc aaagctgtta ctaaagagca gttgcaagcg cttggagtga
     2041 atgcagaaaa tcctcctgca tatatctcaa gtgtggcata tggccgtcaa gtttatttga
     2101 aattatcaac taattcccat agtaccaaag taaaagctgc ttttgacgct gccgtaagtg
     2161 ggaaatctgt ctcaggtgat gtagaactga caaatatcat caaaaattct tccttcaaag
     2221 ccgtaattta cggtggctcc gcaaaagatg aagttcaaat catcgacggt aacctcggag
     2281 acttacgaga tattttgaaa aaaggtgcta cttttaaccg ggaaacacca ggagttccca
     2341 ttgcctatac aacaaacttc ttaaaagaca atgaattagc tgttattaaa aacaactcag
     2401 aatatattga aacaacttca aaagcttata cagatggaaa aatcaacatc gatcactctg
     2461 gaggatacgt tgctcaattc aacatctctt gggatgaaat aaattatgat cctgaaggta
     2521 acgaaattgt tcaacataaa aactggagcg aaaacaataa aagtaagcta gctcatttca
     2581 catcgtccat ctatttgcca ggtaacgcaa gaaatattaa tgtttacgct aaagaatgca
     2641 ctggtttagc ttgggaatgg tggagaacgg taattgatga ccggaaccta ccgcttgtga
     2701 aaaatagaaa tatctccatc tggggcacta cactttatcc gaaatatagt aatagtgtag
     2761 ataatccaat cgaataa
//