LOCUS BBa_K624058 3697 bp DNA linear 22-Dec-2024 DEFINITION pYMB essentials + RBS(Pmsp3) + Inv ACCESSION BBa_K624058 VERSION BBa_K624058.1 FEATURES Location/Qualifiers misc_feature 1..80 /label=ori (pMGT) promoter 81..274 /label=Pmsp1 misc_feature 275..277 /label=start promoter 1..277 /label=BBa_K624000 CDS 313..1142 /label=rep (pMGT) RBS 286..309 /label=rbs from pMGT misc_feature 310..312 /label=start CDS 286..1142 /label=BBa_K624003 CDS 1..1142 /label=BBa_K624004 RBS 1151..1178 /label=rbs (from Pmsp3) misc_feature 1179..1181 /label=artificial start codon RBS 1151..1181 /label=BBa_K624012 promoter 1..1181 /label=BBa_K624028 CDS 1190..4015 /label=protein CDS 1190..3697 /label=BBa_K177010 ORIGIN 1 tgatccgcca gcgccgaagc catcataact gcattgaata attcaaagtg cattgaataa 61 ggcagggtgc ataaggcgca gcggtaacgc cgcctcgccg gtggaagggt gttgtgtggc 121 atccatgcca cagtgcggag ttttcgatac agccgacgtc ccgcctcgct tgactggcgg 181 cgcccatcat ggcgtgatgg cgaccgttcg ttcatttgga tagcggtcgc ggccattggg 241 ggtcgttttc gcaaagccaa agagaggaga taccatgtac tagagcggga gagggagctg 301 atcccgctga tggtgacgct gacaggccat cacaatgcac ggatgagcct cgctgaatgt 361 ctgacggtga tgaagaaggc caagcagagc cttcaccagt ctggcccatg gaagcgccat 421 gttggcccgg tggtcgaagg gcacgtcacg gcaacggagg tcaatcggtc acgccgccat 481 ggctggcacg tccattttca cgtcctgatg ctgattgagg ccgccagcga ggccgaagcg 541 attgcgctcg tgacccgaca cctcgaacct gaatggctcc ggcagcttga gcgccaaggc 601 agctggggcg gcgaacatgc gttagacgtc cagggcgcag ccgatgcgga tcggtatgtg 661 gccaagtggg gcgcggctga ggaattggct ctgtcgggcg aaaagcgggc gcgcggcgac 721 gccgaaatta agggtcaaac gccgtgggaa ttgttggccg ctgcggctga tggtgacgtc 781 caggctggcc gcgattttgt ggagtacgcg accctgtttc atggcaaacg ccagctcgtt 841 tggtcgcgag gactgaaaga gcgtgtcggc ctcaaggtgg tggaagacga cgaggccgcc 901 ggcgagctgg aggatgtcgc agatagcgcc atcaacgaag tggccgtgtt gccgaggccg 961 acttggcgtc acatcgtccg gcgcaggctc cgcgcggatc tgctggccgc cgctcgtgat 1021 gacggattcg atggcgtcgc gcggctgctc aatgagcatg gcatcggcgg agctatgccc 1081 ggcggctggc gaccaatgct tagcgaatat accgagcata ttgcgatggc tgcgtgactc 1141 tttactagag gtccaagccc tatgagggag cctttctaat gtactagagc tattgaggct 1201 ccgcacacag cggatacgcc gtagccgcag atgttggtat gtcgccggtg gtcatatcca 1261 tagtgacaaa atctgtactg gtctttttag tccaatagtt acccgatggc cactcagcgc 1321 tatcataggt tgccaaactt ccccactctc cccacagagt cccgtctggt gaacgcgaac 1381 cattactggc gcgtgcggac tcgatcagcg cagtaaaatc agtgcgctgg caatttcgac 1441 tcgcctcgac acttgattgc agtgacatgg tgccggagaa cacccacaaa ttaggtttga 1501 attgatatgt tgccgtataa ctcgggaatt ttttactttt tgccgtcaca gtgacggtgc 1561 taccataggt tttataggca atattgactt taccctgatt atcaaccggc gcactggccg 1621 cataggatga tgtccagtca tattgagtat tattcgccac atcgtcattc atcaccaatt 1681 ggaacgtggc tttattaaag gtagttttcg ggaagccttt atctgtggca aattgctcac 1741 cattcacctt aatgccggtt atcttcggta ctgggtacag ggtgattctt ttctgcaaca 1801 agtctagtga ttcccccccc acctgcggcg taatatcgac atctcctacg gaatttccca 1861 ccacactggc ggtatagttg tcagcatttt cggttaccga actaatagtt accggaacac 1921 cactttgtat aaattcaaga tctgttatcc cactgacaaa ctcattattt ttattacgcg 1981 gaacaaaggt gagtatcgac tgcatggagc catcggcaac aatatcagat ggagaaacgt 2041 taaaacttga gtttgtgaca tctggcggca gcacggcaaa tcgtacatca acagtagcct 2101 gttgcccctg aatatctaaa gtgacgactg acaccccaac agtgaagcta gttaatgcaa 2161 tgcttatcac accctgtgca tcggtttttt ttgtgacttt actgggaagc gcaccattac 2221 tggtggttat caccccctct tgctcggcaa ccggcgtact atcaatatta gtcactgtaa 2281 atttcaccgt gatgggagtc ctgccatcag caagcgcacc atcatcagtc acttccgagg 2341 taataacgaa ctgaggttgc tgcacaataa cggtcaacgt agctggatta gaactattgc 2401 cgtggttatc gatagccgtg gcgctgaggg tataggtatt gaccgccgct tcgctctcgc 2461 cggttttccc cacagtacga ctcgctggtg ccatcggttt ataaggcggt aagaccacat 2521 tgtaatctgt tgcactgacg ggtattaatt tgccgccggc agcaacccat tgcgcatcat 2581 tccagagaat acgttgtagt gcagattgag actgtatttg tgcactaacg gaataaacct 2641 gccccggcag gccggagagt acggcgggtg aaaatgccaa tttgatagta ttctgttttt 2701 ggtattccag aacaatattt ggattgcgct caaccagatt ataacggctc tcagccagta 2761 aacgagttcc ggccaccact gcggggctaa actgcgaacg aaaactttcg ccgaggcgat 2821 aatccatttg taagttccat tgtatttcat gctgccgact ttttcccatt cgttgatcga 2881 cccccagtgt aatgaagggg atcggcgtat aaataagccc tgtggtcacc gcataagggt 2941 tactttgcag gttatcttta ccaaataaag ccacccgctc accacggtac tgctcatatt 3001 ttaatttccc gcccaattgt ggcagcgcag gtaaatacgc tttgacgtga atgtcgcccc 3061 cgcttgccgg gcgctcatta tagtccgcga aatcacgaga ttgatgccaa ccattgaggc 3121 gaaaataacc attggccgat aattgtaaat aatcagtcca ggcttctgca cccacgccaa 3181 tacgatgatt gtgcccggtc atatcattgt catagaaagt gttaaagcca tacatccaac 3241 tttgttggaa ggtacgcacc ccagcgccga tattaagggt attgcgactg tctttatttc 3301 ttatacccaa ctgactaaaa aagacatatg aagcagagtc ataccaaggc aacagccaat 3361 caagagaact ttctttgagg gagaactttt tatcaaagtt aacattgacc tgagttgtcc 3421 caaatctatt taaccagtgt tttacttcct gatttgccgc gtcatttacc attgacctgg 3481 tgatattgga tgcattaccc gataccgcca gactggttcc ggtactgatt atcttattga 3541 aagcctcatt atgttcagcg gggttctcta acgcttctga attaaaataa tgttgctgtg 3601 agaacccata agtaaatatt ccaaatatta aaccgatcga taatattagc ctgctaatta 3661 ttttagtcac agttagcgta ttaaaaaatg aatacat //