LOCUS       BBa_K624060             1877 bp    DNA     linear       12-Mar-2025
DEFINITION  pYMB essentials + RBS(trunc.) + minC
ACCESSION   BBa_K624060
VERSION     BBa_K624060.1  
FEATURES             Location/Qualifiers
     misc_feature    1..80
                     /label=ori (pMGT)
     promoter        81..274
                     /label=Pmsp1
     misc_feature    275..277
                     /label=start
     promoter        1..277
                     /label=BBa_K624000
     CDS             313..1142
                     /label=rep (pMGT)
     RBS             286..309
                     /label=rbs from pMGT
     misc_feature    310..312
                     /label=start
     CDS             286..1142
                     /label=BBa_K624003
     CDS             1..1142
                     /label=BBa_K624004
     RBS             1151..1172
                     /label=rbs (trunc.) from Pmsp3
     RBS             1151..1172
                     /label=BBa_K624013
     promoter        1..1172
                     /label=BBa_K624029
     CDS             1179..1877
                     /label=minC
     primer_bind     1857..1877
                     /label=reverse minC primer binding
     primer_bind     1179..1206
                     /label=forward minC primer binding
     CDS             1179..1877
                     /label=BBa_K624033
ORIGIN
        1 tgatccgcca gcgccgaagc catcataact gcattgaata attcaaagtg cattgaataa
       61 ggcagggtgc ataaggcgca gcggtaacgc cgcctcgccg gtggaagggt gttgtgtggc
      121 atccatgcca cagtgcggag ttttcgatac agccgacgtc ccgcctcgct tgactggcgg
      181 cgcccatcat ggcgtgatgg cgaccgttcg ttcatttgga tagcggtcgc ggccattggg
      241 ggtcgttttc gcaaagccaa agagaggaga taccatgtac tagagcggga gagggagctg
      301 atcccgctga tggtgacgct gacaggccat cacaatgcac ggatgagcct cgctgaatgt
      361 ctgacggtga tgaagaaggc caagcagagc cttcaccagt ctggcccatg gaagcgccat
      421 gttggcccgg tggtcgaagg gcacgtcacg gcaacggagg tcaatcggtc acgccgccat
      481 ggctggcacg tccattttca cgtcctgatg ctgattgagg ccgccagcga ggccgaagcg
      541 attgcgctcg tgacccgaca cctcgaacct gaatggctcc ggcagcttga gcgccaaggc
      601 agctggggcg gcgaacatgc gttagacgtc cagggcgcag ccgatgcgga tcggtatgtg
      661 gccaagtggg gcgcggctga ggaattggct ctgtcgggcg aaaagcgggc gcgcggcgac
      721 gccgaaatta agggtcaaac gccgtgggaa ttgttggccg ctgcggctga tggtgacgtc
      781 caggctggcc gcgattttgt ggagtacgcg accctgtttc atggcaaacg ccagctcgtt
      841 tggtcgcgag gactgaaaga gcgtgtcggc ctcaaggtgg tggaagacga cgaggccgcc
      901 ggcgagctgg aggatgtcgc agatagcgcc atcaacgaag tggccgtgtt gccgaggccg
      961 acttggcgtc acatcgtccg gcgcaggctc cgcgcggatc tgctggccgc cgctcgtgat
     1021 gacggattcg atggcgtcgc gcggctgctc aatgagcatg gcatcggcgg agctatgccc
     1081 ggcggctggc gaccaatgct tagcgaatat accgagcata ttgcgatggc tgcgtgactc
     1141 tttactagag gtccaagccc tatgagggag cctactagat gtcaaacacg ccaatcgagc
     1201 ttaaaggcag tagcttcact ttatctgtgg ttcatctgca tgaggcagaa cctaaggtta
     1261 tccatcaggc gctggaagac aaaatcgctc aggcccccgc atttttaaaa catgcccccg
     1321 ttgtactcaa cgtcagtgca ctggaagacc cggtaaactg gtcagcgatg cataaggcgg
     1381 tttcggcaac cggtttgcgg gttattggcg taagtggctg caaagatgcg caacttaaag
     1441 ccgaaattga aaagatgggg ctgcctatcc tgacggaagg aaaggaaaaa gcgccacgtc
     1501 cagctcccac accgcaggct ccagcgcaaa atacaacgcc ggtcacaaaa acgcgtttaa
     1561 tagatacccc ggtgcgttcc ggtcagcgta tttatgctcc acaatgtgat ctgattgtta
     1621 caagccacgt tagcgctggg gccgaattga ttgccgatgg gaacattcat gtctatggca
     1681 tgatgcgcgg tcgtgcgctg gcaggggcaa gtggtgaccg ggaaacgcaa atattttgta
     1741 cgaacctgat ggcggaactg gtgtccatcg caggtgaata ctggctgagt gatcaaatcc
     1801 cagcagaatt ttatggcaaa gcggcgcgac tgcaattagt cgaaaacgct ttgaccgttc
     1861 aaccgttaaa ttaataa
//