LOCUS       BBa_K624061             2768 bp    DNA     linear       26-Nov-2024
DEFINITION  pYMB essentials + RBS(trunc.) + LLO
ACCESSION   BBa_K624061
VERSION     BBa_K624061.1  
FEATURES             Location/Qualifiers
     misc_feature    1..80
                     /label=ori (pMGT)
     promoter        81..274
                     /label=Pmsp1
     misc_feature    275..277
                     /label=start
     promoter        1..277
                     /label=BBa_K624000
     CDS             313..1142
                     /label=rep (pMGT)
     RBS             286..309
                     /label=rbs from pMGT
     misc_feature    310..312
                     /label=start
     CDS             286..1142
                     /label=BBa_K624003
     CDS             1..1142
                     /label=BBa_K624004
     RBS             1151..1172
                     /label=rbs (trunc.) from Pmsp3
     RBS             1151..1172
                     /label=BBa_K624013
     promoter        1..1172
                     /label=BBa_K624029
     CDS             1179..2768
                     /label=protein
     CDS             1179..2768
                     /label=BBa_K177026
ORIGIN
        1 tgatccgcca gcgccgaagc catcataact gcattgaata attcaaagtg cattgaataa
       61 ggcagggtgc ataaggcgca gcggtaacgc cgcctcgccg gtggaagggt gttgtgtggc
      121 atccatgcca cagtgcggag ttttcgatac agccgacgtc ccgcctcgct tgactggcgg
      181 cgcccatcat ggcgtgatgg cgaccgttcg ttcatttgga tagcggtcgc ggccattggg
      241 ggtcgttttc gcaaagccaa agagaggaga taccatgtac tagagcggga gagggagctg
      301 atcccgctga tggtgacgct gacaggccat cacaatgcac ggatgagcct cgctgaatgt
      361 ctgacggtga tgaagaaggc caagcagagc cttcaccagt ctggcccatg gaagcgccat
      421 gttggcccgg tggtcgaagg gcacgtcacg gcaacggagg tcaatcggtc acgccgccat
      481 ggctggcacg tccattttca cgtcctgatg ctgattgagg ccgccagcga ggccgaagcg
      541 attgcgctcg tgacccgaca cctcgaacct gaatggctcc ggcagcttga gcgccaaggc
      601 agctggggcg gcgaacatgc gttagacgtc cagggcgcag ccgatgcgga tcggtatgtg
      661 gccaagtggg gcgcggctga ggaattggct ctgtcgggcg aaaagcgggc gcgcggcgac
      721 gccgaaatta agggtcaaac gccgtgggaa ttgttggccg ctgcggctga tggtgacgtc
      781 caggctggcc gcgattttgt ggagtacgcg accctgtttc atggcaaacg ccagctcgtt
      841 tggtcgcgag gactgaaaga gcgtgtcggc ctcaaggtgg tggaagacga cgaggccgcc
      901 ggcgagctgg aggatgtcgc agatagcgcc atcaacgaag tggccgtgtt gccgaggccg
      961 acttggcgtc acatcgtccg gcgcaggctc cgcgcggatc tgctggccgc cgctcgtgat
     1021 gacggattcg atggcgtcgc gcggctgctc aatgagcatg gcatcggcgg agctatgccc
     1081 ggcggctggc gaccaatgct tagcgaatat accgagcata ttgcgatggc tgcgtgactc
     1141 tttactagag gtccaagccc tatgagggag cctactagat gaaaaaaata atgctagttt
     1201 ttattacact tatattagtt agtctaccaa ttgcgcaaca aactgaagca aaggatgcat
     1261 ctgcattcaa taaagaaaat ttaatttcat ccatagcacc accagcatct ccgcctgcaa
     1321 gtcctaagac gccaatcgaa aagaaacacg cggatgaaat cgataagtat atacaaggat
     1381 tggattacaa taaaaacaat gtattagtat accacggaga tgcagtgaca aatgtgccgc
     1441 caagaaaagg ttataaagat ggaaatgaat atatcgttgt ggagaaaaag aagaaatcca
     1501 tcaatcaaaa taatgcagat atccaagttg tgaatgcaat ttcgagccta acatatccag
     1561 gtgctctcgt gaaagccaat tcggaattag tagaaaatca acccgatgtt cttcctgtca
     1621 aacgtgattc attaacactt agcattgatt tgccaggaat gactaatcaa gacaataaaa
     1681 ttgttgtaaa aaatgctact aaatcgaacg ttaacaacgc agtaaataca ttagtggaaa
     1741 gatggaatga aaaatatgct caagcttatc caaatgtaag tgcaaaaatt gattatgatg
     1801 acgaaatggc ttacagtgaa tcacaattaa ttgcaaaatt tggtacggca tttaaagctg
     1861 taaataatag cttgaatgta aacttcggcg caatcagtga agggaaaatg caagaagaag
     1921 tcattagttt taaacaaatt tactataacg tgaatgttaa tgaacctaca agaccttcca
     1981 gatttttcgg caaagctgtt actaaagagc agttgcaagc gcttggagtg aatgcagaaa
     2041 atcctcctgc atatatctca agtgtggcat atggccgtca agtttatttg aaattatcaa
     2101 ctaattccca tagtaccaaa gtaaaagctg cttttgacgc tgccgtaagt gggaaatctg
     2161 tctcaggtga tgtagaactg acaaatatca tcaaaaattc ttccttcaaa gccgtaattt
     2221 acggtggctc cgcaaaagat gaagttcaaa tcatcgacgg taacctcgga gacttacgag
     2281 atattttgaa aaaaggtgct acttttaacc gggaaacacc aggagttccc attgcctata
     2341 caacaaactt cttaaaagac aatgaattag ctgttattaa aaacaactca gaatatattg
     2401 aaacaacttc aaaagcttat acagatggaa aaatcaacat cgatcactct ggaggatacg
     2461 ttgctcaatt caacatctct tgggatgaaa taaattatga tcctgaaggt aacgaaattg
     2521 ttcaacataa aaactggagc gaaaacaata aaagtaagct agctcatttc acatcgtcca
     2581 tctatttgcc aggtaacgca agaaatatta atgtttacgc taaagaatgc actggtttag
     2641 cttgggaatg gtggagaacg gtaattgatg accggaacct accgcttgtg aaaaatagaa
     2701 atatctccat ctggggcact acactttatc cgaaatatag taatagtgta gataatccaa
     2761 tcgaataa
//