LOCUS BBa_K624062 3688 bp DNA linear 29-Nov-2024 DEFINITION pYMB essentials + RBS(trunc.) + Inv ACCESSION BBa_K624062 VERSION BBa_K624062.1 FEATURES Location/Qualifiers misc_feature 1..80 /label=ori (pMGT) promoter 81..274 /label=Pmsp1 misc_feature 275..277 /label=start promoter 1..277 /label=BBa_K624000 CDS 313..1142 /label=rep (pMGT) RBS 286..309 /label=rbs from pMGT misc_feature 310..312 /label=start CDS 286..1142 /label=BBa_K624003 CDS 1..1142 /label=BBa_K624004 RBS 1151..1172 /label=rbs (trunc.) from Pmsp3 RBS 1151..1172 /label=BBa_K624013 promoter 1..1172 /label=BBa_K624029 CDS 1181..4006 /label=protein CDS 1181..3688 /label=BBa_K177010 ORIGIN 1 tgatccgcca gcgccgaagc catcataact gcattgaata attcaaagtg cattgaataa 61 ggcagggtgc ataaggcgca gcggtaacgc cgcctcgccg gtggaagggt gttgtgtggc 121 atccatgcca cagtgcggag ttttcgatac agccgacgtc ccgcctcgct tgactggcgg 181 cgcccatcat ggcgtgatgg cgaccgttcg ttcatttgga tagcggtcgc ggccattggg 241 ggtcgttttc gcaaagccaa agagaggaga taccatgtac tagagcggga gagggagctg 301 atcccgctga tggtgacgct gacaggccat cacaatgcac ggatgagcct cgctgaatgt 361 ctgacggtga tgaagaaggc caagcagagc cttcaccagt ctggcccatg gaagcgccat 421 gttggcccgg tggtcgaagg gcacgtcacg gcaacggagg tcaatcggtc acgccgccat 481 ggctggcacg tccattttca cgtcctgatg ctgattgagg ccgccagcga ggccgaagcg 541 attgcgctcg tgacccgaca cctcgaacct gaatggctcc ggcagcttga gcgccaaggc 601 agctggggcg gcgaacatgc gttagacgtc cagggcgcag ccgatgcgga tcggtatgtg 661 gccaagtggg gcgcggctga ggaattggct ctgtcgggcg aaaagcgggc gcgcggcgac 721 gccgaaatta agggtcaaac gccgtgggaa ttgttggccg ctgcggctga tggtgacgtc 781 caggctggcc gcgattttgt ggagtacgcg accctgtttc atggcaaacg ccagctcgtt 841 tggtcgcgag gactgaaaga gcgtgtcggc ctcaaggtgg tggaagacga cgaggccgcc 901 ggcgagctgg aggatgtcgc agatagcgcc atcaacgaag tggccgtgtt gccgaggccg 961 acttggcgtc acatcgtccg gcgcaggctc cgcgcggatc tgctggccgc cgctcgtgat 1021 gacggattcg atggcgtcgc gcggctgctc aatgagcatg gcatcggcgg agctatgccc 1081 ggcggctggc gaccaatgct tagcgaatat accgagcata ttgcgatggc tgcgtgactc 1141 tttactagag gtccaagccc tatgagggag cctactagag ctattgaggc tccgcacaca 1201 gcggatacgc cgtagccgca gatgttggta tgtcgccggt ggtcatatcc atagtgacaa 1261 aatctgtact ggtcttttta gtccaatagt tacccgatgg ccactcagcg ctatcatagg 1321 ttgccaaact tccccactct ccccacagag tcccgtctgg tgaacgcgaa ccattactgg 1381 cgcgtgcgga ctcgatcagc gcagtaaaat cagtgcgctg gcaatttcga ctcgcctcga 1441 cacttgattg cagtgacatg gtgccggaga acacccacaa attaggtttg aattgatatg 1501 ttgccgtata actcgggaat tttttacttt ttgccgtcac agtgacggtg ctaccatagg 1561 ttttataggc aatattgact ttaccctgat tatcaaccgg cgcactggcc gcataggatg 1621 atgtccagtc atattgagta ttattcgcca catcgtcatt catcaccaat tggaacgtgg 1681 ctttattaaa ggtagttttc gggaagcctt tatctgtggc aaattgctca ccattcacct 1741 taatgccggt tatcttcggt actgggtaca gggtgattct tttctgcaac aagtctagtg 1801 attccccccc cacctgcggc gtaatatcga catctcctac ggaatttccc accacactgg 1861 cggtatagtt gtcagcattt tcggttaccg aactaatagt taccggaaca ccactttgta 1921 taaattcaag atctgttatc ccactgacaa actcattatt tttattacgc ggaacaaagg 1981 tgagtatcga ctgcatggag ccatcggcaa caatatcaga tggagaaacg ttaaaacttg 2041 agtttgtgac atctggcggc agcacggcaa atcgtacatc aacagtagcc tgttgcccct 2101 gaatatctaa agtgacgact gacaccccaa cagtgaagct agttaatgca atgcttatca 2161 caccctgtgc atcggttttt tttgtgactt tactgggaag cgcaccatta ctggtggtta 2221 tcaccccctc ttgctcggca accggcgtac tatcaatatt agtcactgta aatttcaccg 2281 tgatgggagt cctgccatca gcaagcgcac catcatcagt cacttccgag gtaataacga 2341 actgaggttg ctgcacaata acggtcaacg tagctggatt agaactattg ccgtggttat 2401 cgatagccgt ggcgctgagg gtataggtat tgaccgccgc ttcgctctcg ccggttttcc 2461 ccacagtacg actcgctggt gccatcggtt tataaggcgg taagaccaca ttgtaatctg 2521 ttgcactgac gggtattaat ttgccgccgg cagcaaccca ttgcgcatca ttccagagaa 2581 tacgttgtag tgcagattga gactgtattt gtgcactaac ggaataaacc tgccccggca 2641 ggccggagag tacggcgggt gaaaatgcca atttgatagt attctgtttt tggtattcca 2701 gaacaatatt tggattgcgc tcaaccagat tataacggct ctcagccagt aaacgagttc 2761 cggccaccac tgcggggcta aactgcgaac gaaaactttc gccgaggcga taatccattt 2821 gtaagttcca ttgtatttca tgctgccgac tttttcccat tcgttgatcg acccccagtg 2881 taatgaaggg gatcggcgta taaataagcc ctgtggtcac cgcataaggg ttactttgca 2941 ggttatcttt accaaataaa gccacccgct caccacggta ctgctcatat tttaatttcc 3001 cgcccaattg tggcagcgca ggtaaatacg ctttgacgtg aatgtcgccc ccgcttgccg 3061 ggcgctcatt atagtccgcg aaatcacgag attgatgcca accattgagg cgaaaataac 3121 cattggccga taattgtaaa taatcagtcc aggcttctgc acccacgcca atacgatgat 3181 tgtgcccggt catatcattg tcatagaaag tgttaaagcc atacatccaa ctttgttgga 3241 aggtacgcac cccagcgccg atattaaggg tattgcgact gtctttattt cttataccca 3301 actgactaaa aaagacatat gaagcagagt cataccaagg caacagccaa tcaagagaac 3361 tttctttgag ggagaacttt ttatcaaagt taacattgac ctgagttgtc ccaaatctat 3421 ttaaccagtg ttttacttcc tgatttgccg cgtcatttac cattgacctg gtgatattgg 3481 atgcattacc cgataccgcc agactggttc cggtactgat tatcttattg aaagcctcat 3541 tatgttcagc ggggttctct aacgcttctg aattaaaata atgttgctgt gagaacccat 3601 aagtaaatat tccaaatatt aaaccgatcg ataatattag cctgctaatt attttagtca 3661 cagttagcgt attaaaaaat gaatacat //