LOCUS BBa_K624067 1967 bp DNA linear 25-Nov-2024 DEFINITION pYMB+|CHAMP-EYFP| ACCESSION BBa_K624067 VERSION BBa_K624067.1 FEATURES Location/Qualifiers misc_feature 1..80 /label=ori (pMGT) promoter 81..274 /label=Pmsp1 misc_feature 275..277 /label=start promoter 1..277 /label=BBa_K624000 CDS 313..1142 /label=rep (pMGT) RBS 286..309 /label=rbs from pMGT misc_feature 310..312 /label=start CDS 286..1142 /label=BBa_K624003 CDS 1..1142 /label=BBa_K624004 RBS 1151..1178 /label=rbs (from Pmsp3) misc_feature 1179..1181 /label=artificial start codon RBS 1151..1181 /label=BBa_K624012 promoter 1..1181 /label=BBa_K624028 misc_feature 1188..1244 /label=BBa_K624010 misc_feature 1188..1190 /label=start CDS 1191..1244 /label=CHAMP (anti-H1[mms13]) misc_feature 1245..1967 /label=BBa_E0030 CDS 1188..1967 /label=BBa_K624025 ORIGIN 1 tgatccgcca gcgccgaagc catcataact gcattgaata attcaaagtg cattgaataa 61 ggcagggtgc ataaggcgca gcggtaacgc cgcctcgccg gtggaagggt gttgtgtggc 121 atccatgcca cagtgcggag ttttcgatac agccgacgtc ccgcctcgct tgactggcgg 181 cgcccatcat ggcgtgatgg cgaccgttcg ttcatttgga tagcggtcgc ggccattggg 241 ggtcgttttc gcaaagccaa agagaggaga taccatgtac tagagcggga gagggagctg 301 atcccgctga tggtgacgct gacaggccat cacaatgcac ggatgagcct cgctgaatgt 361 ctgacggtga tgaagaaggc caagcagagc cttcaccagt ctggcccatg gaagcgccat 421 gttggcccgg tggtcgaagg gcacgtcacg gcaacggagg tcaatcggtc acgccgccat 481 ggctggcacg tccattttca cgtcctgatg ctgattgagg ccgccagcga ggccgaagcg 541 attgcgctcg tgacccgaca cctcgaacct gaatggctcc ggcagcttga gcgccaaggc 601 agctggggcg gcgaacatgc gttagacgtc cagggcgcag ccgatgcgga tcggtatgtg 661 gccaagtggg gcgcggctga ggaattggct ctgtcgggcg aaaagcgggc gcgcggcgac 721 gccgaaatta agggtcaaac gccgtgggaa ttgttggccg ctgcggctga tggtgacgtc 781 caggctggcc gcgattttgt ggagtacgcg accctgtttc atggcaaacg ccagctcgtt 841 tggtcgcgag gactgaaaga gcgtgtcggc ctcaaggtgg tggaagacga cgaggccgcc 901 ggcgagctgg aggatgtcgc agatagcgcc atcaacgaag tggccgtgtt gccgaggccg 961 acttggcgtc acatcgtccg gcgcaggctc cgcgcggatc tgctggccgc cgctcgtgat 1021 gacggattcg atggcgtcgc gcggctgctc aatgagcatg gcatcggcgg agctatgccc 1081 ggcggctggc gaccaatgct tagcgaatat accgagcata ttgcgatggc tgcgtgactc 1141 tttactagag gtccaagccc tatgagggag cctttctaat gtactagatg aagaagctgt 1201 tcgtgctgct gatgttcatc atcgtggccc tgctgtggaa gaagatggtg agcaagggcg 1261 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc 1321 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga 1381 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttcg 1441 gctacggcct gcaatgcttc gcccgctacc ccgaccacat gaagctgcac gacttcttca 1501 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca 1561 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc 1621 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact 1681 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact 1741 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga 1801 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt 1861 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga 1921 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaataa //