BBa_K625000
1
BBa_K625000
RBS-LacIM1
2011-09-20T11:00:00Z
2015-05-08T01:12:56Z
PCR on DNA synthesized by GeneArt
Contains a rather weak RBS in front of a codon modified variant of LacI. This reduces the likelihood of recombination with wild type LacI when used in multi plasmid systems.
false
false
_797_
0
10153
9
It's complicated
false
The RBS was chosen to match the one in [Basu et al, 2005] in order to create a working band pass filter. Minimal RBS length was chosen to be 40 bp in accordance with [Salis et al, 2009].
false
Michael Eichenberger
annotation2136635
1
RBS
range2136635
1
1
35
annotation2136636
1
LacI M1
range2136636
1
36
1121
BBa_K625000_sequence
1
tatagtcgaataaaacaagtggctaaggaggttgtatgaagccggtgaccctgtatgacgtggcggaatacgcgggcgtgagctaccaaacggtgagccgtgttgttaatcaagcgtctcatgtgagcgccaagacccgcgagaaggttgaggccgccatggccgaattaaactatatcccgaatcgtgttgcgcagcagttagccggtaagcaaagcctgttaatcggtgtggcgacgagcagcttagcgttacatgccccaagccagatcgtggccgccatcaagagccgtgcggaccagttaggcgcgtctgttgttgttagcatggtggagcgctctggtgtggaggcgtgcaaggccgccgttcataacctgctggcccagcgtgtgagcggcttaattatcaattacccattagacgatcaagacgcgatcgcggttgaggcggcgtgtaccaacgtgccagccctgttcctggacgtgagcgatcaaaccccgattaatagcatcatctttagccacgaggatggcacccgcttaggtgttgaacacttagtggcgctgggccatcaacagattgccttactggccggtccgctgagcagcgtgagcgcccgcttacgcttagcgggttggcacaagtacctgacccgtaaccagatccaaccaattgccgagcgcgagggtgattggagcgcgatgagcggcttccagcagacgatgcagatgttaaacgaaggtattgtgccgaccgccatgttagtggcgaatgaccaaatggccttaggtgcgatgcgtgcgatcacggaaagcggcttacgtgtgggcgccgacattagcgtggttggctatgatgacacggaggattctagctgctacattccaccaagcacgacgattaagcaagacttccgtttattaggccagacgtctgttgatcgtctgttacagctgagccaaggtcaagccgttaaaggtaaccaattactgccggtgagcttagttaagcgcaagacgacgttagccccgaacacccagacggcgagcccgcgtgccctggcggacagcctgatgcaattagcgcgccaagtgagccgcttagagtctggccaataataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z