BBa_K625000 1 BBa_K625000 RBS-LacIM1 2011-09-20T11:00:00Z 2015-05-08T01:12:56Z PCR on DNA synthesized by GeneArt Contains a rather weak RBS in front of a codon modified variant of LacI. This reduces the likelihood of recombination with wild type LacI when used in multi plasmid systems. false false _797_ 0 10153 9 It's complicated false The RBS was chosen to match the one in [Basu et al, 2005] in order to create a working band pass filter. Minimal RBS length was chosen to be 40 bp in accordance with [Salis et al, 2009]. false Michael Eichenberger annotation2136636 1 LacI M1 range2136636 1 36 1121 annotation2136635 1 RBS range2136635 1 1 35 BBa_K625000_sequence 1 tatagtcgaataaaacaagtggctaaggaggttgtatgaagccggtgaccctgtatgacgtggcggaatacgcgggcgtgagctaccaaacggtgagccgtgttgttaatcaagcgtctcatgtgagcgccaagacccgcgagaaggttgaggccgccatggccgaattaaactatatcccgaatcgtgttgcgcagcagttagccggtaagcaaagcctgttaatcggtgtggcgacgagcagcttagcgttacatgccccaagccagatcgtggccgccatcaagagccgtgcggaccagttaggcgcgtctgttgttgttagcatggtggagcgctctggtgtggaggcgtgcaaggccgccgttcataacctgctggcccagcgtgtgagcggcttaattatcaattacccattagacgatcaagacgcgatcgcggttgaggcggcgtgtaccaacgtgccagccctgttcctggacgtgagcgatcaaaccccgattaatagcatcatctttagccacgaggatggcacccgcttaggtgttgaacacttagtggcgctgggccatcaacagattgccttactggccggtccgctgagcagcgtgagcgcccgcttacgcttagcgggttggcacaagtacctgacccgtaaccagatccaaccaattgccgagcgcgagggtgattggagcgcgatgagcggcttccagcagacgatgcagatgttaaacgaaggtattgtgccgaccgccatgttagtggcgaatgaccaaatggccttaggtgcgatgcgtgcgatcacggaaagcggcttacgtgtgggcgccgacattagcgtggttggctatgatgacacggaggattctagctgctacattccaccaagcacgacgattaagcaagacttccgtttattaggccagacgtctgttgatcgtctgttacagctgagccaaggtcaagccgttaaaggtaaccaattactgccggtgagcttagttaagcgcaagacgacgttagccccgaacacccagacggcgagcccgcgtgccctggcggacagcctgatgcaattagcgcgccaagtgagccgcttagagtctggccaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z