LOCUS       BBa_K625001             1312 bp    DNA     linear       14-Jun-2024
DEFINITION  pTet - LacIM1 - Ter
ACCESSION   BBa_K625001
VERSION     BBa_K625001.1  
FEATURES             Location/Qualifiers
     misc_feature    1..54
                     /label=BBa_R0040
     misc_feature    1..19
                     /label=TetR 1
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     RBS             55..89
                     /label=RBS
     CDS             90..1175
                     /label=LacI M1
     misc_feature    55..1175
                     /label=BBa_K625000
     misc_feature    1184..1263
                     /label=BBa_B0010
     stem_loop       1195..1238
                     /label=stem_loop
     misc_feature    1272..1312
                     /label=BBa_B0012
     stem_loop       1279..1298
                     /label=T7 TE
     polyA_site      1299..1312
                     /label=polya
     misc_feature    1305..1305
                     /label=stop
     misc_feature    1184..1312
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactatagt
       61 cgaataaaac aagtggctaa ggaggttgta tgaagccggt gaccctgtat gacgtggcgg
      121 aatacgcggg cgtgagctac caaacggtga gccgtgttgt taatcaagcg tctcatgtga
      181 gcgccaagac ccgcgagaag gttgaggccg ccatggccga attaaactat atcccgaatc
      241 gtgttgcgca gcagttagcc ggtaagcaaa gcctgttaat cggtgtggcg acgagcagct
      301 tagcgttaca tgccccaagc cagatcgtgg ccgccatcaa gagccgtgcg gaccagttag
      361 gcgcgtctgt tgttgttagc atggtggagc gctctggtgt ggaggcgtgc aaggccgccg
      421 ttcataacct gctggcccag cgtgtgagcg gcttaattat caattaccca ttagacgatc
      481 aagacgcgat cgcggttgag gcggcgtgta ccaacgtgcc agccctgttc ctggacgtga
      541 gcgatcaaac cccgattaat agcatcatct ttagccacga ggatggcacc cgcttaggtg
      601 ttgaacactt agtggcgctg ggccatcaac agattgcctt actggccggt ccgctgagca
      661 gcgtgagcgc ccgcttacgc ttagcgggtt ggcacaagta cctgacccgt aaccagatcc
      721 aaccaattgc cgagcgcgag ggtgattgga gcgcgatgag cggcttccag cagacgatgc
      781 agatgttaaa cgaaggtatt gtgccgaccg ccatgttagt ggcgaatgac caaatggcct
      841 taggtgcgat gcgtgcgatc acggaaagcg gcttacgtgt gggcgccgac attagcgtgg
      901 ttggctatga tgacacggag gattctagct gctacattcc accaagcacg acgattaagc
      961 aagacttccg tttattaggc cagacgtctg ttgatcgtct gttacagctg agccaaggtc
     1021 aagccgttaa aggtaaccaa ttactgccgg tgagcttagt taagcgcaag acgacgttag
     1081 ccccgaacac ccagacggcg agcccgcgtg ccctggcgga cagcctgatg caattagcgc
     1141 gccaagtgag ccgcttagag tctggccaat aataatacta gagccaggca tcaaataaaa
     1201 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     1261 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
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