BBa_K625001 1 BBa_K625001 pTet - LacIM1 - Ter 2011-09-20T11:00:00Z 2015-05-08T01:12:56Z Synthesized by GeneArt. Cloned into a BioBrick backbone. Contains RBS-LacIM1 (BBa_K625000) behind the Tet-Promoter (BBa_R0040) false false _797_ 0 10153 9 It's complicated false As we want a multiplasmid system with LacI behind a Tet and a lambda promoter, we used the codon modified LacIM1 from (Basu et al, 2005) to prevent recombination between the two. false Michael Eichenberger annotation2137845 1 BBa_B0015 range2137845 1 1184 1312 annotation2137833 1 -35 range2137833 1 20 25 annotation2137840 1 stem_loop range2137840 1 1195 1238 annotation2137837 1 LacI M1 range2137837 1 90 1175 annotation2137843 1 polya range2137843 1 1299 1312 annotation2137831 1 BBa_R0040 range2137831 1 1 54 annotation2137842 1 T7 TE range2137842 1 1279 1298 annotation2137832 1 TetR 1 range2137832 1 1 19 annotation2137841 1 BBa_B0012 range2137841 1 1272 1312 annotation2137839 1 BBa_B0010 range2137839 1 1184 1263 annotation2137838 1 BBa_K625000 range2137838 1 55 1175 annotation2137844 1 stop range2137844 1 1305 1305 annotation2137834 1 TetR 2 range2137834 1 26 44 annotation2137836 1 RBS range2137836 1 55 89 annotation2137835 1 -10 range2137835 1 43 48 BBa_K625001_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactatagtcgaataaaacaagtggctaaggaggttgtatgaagccggtgaccctgtatgacgtggcggaatacgcgggcgtgagctaccaaacggtgagccgtgttgttaatcaagcgtctcatgtgagcgccaagacccgcgagaaggttgaggccgccatggccgaattaaactatatcccgaatcgtgttgcgcagcagttagccggtaagcaaagcctgttaatcggtgtggcgacgagcagcttagcgttacatgccccaagccagatcgtggccgccatcaagagccgtgcggaccagttaggcgcgtctgttgttgttagcatggtggagcgctctggtgtggaggcgtgcaaggccgccgttcataacctgctggcccagcgtgtgagcggcttaattatcaattacccattagacgatcaagacgcgatcgcggttgaggcggcgtgtaccaacgtgccagccctgttcctggacgtgagcgatcaaaccccgattaatagcatcatctttagccacgaggatggcacccgcttaggtgttgaacacttagtggcgctgggccatcaacagattgccttactggccggtccgctgagcagcgtgagcgcccgcttacgcttagcgggttggcacaagtacctgacccgtaaccagatccaaccaattgccgagcgcgagggtgattggagcgcgatgagcggcttccagcagacgatgcagatgttaaacgaaggtattgtgccgaccgccatgttagtggcgaatgaccaaatggccttaggtgcgatgcgtgcgatcacggaaagcggcttacgtgtgggcgccgacattagcgtggttggctatgatgacacggaggattctagctgctacattccaccaagcacgacgattaagcaagacttccgtttattaggccagacgtctgttgatcgtctgttacagctgagccaaggtcaagccgttaaaggtaaccaattactgccggtgagcttagttaagcgcaagacgacgttagccccgaacacccagacggcgagcccgcgtgccctggcggacagcctgatgcaattagcgcgccaagtgagccgcttagagtctggccaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z