BBa_K625002
1
BBa_K625002
Pu promoter long version with stop codon
2011-09-20T11:00:00Z
2015-05-08T01:12:56Z
PCR reaction on plasmid DNA provided by Prof. Sven Panke. pCK04AxylR
This is an adapted version of the Pu promoter BBa_I723020. The original design contains the RBS and first 81bp of the XylU. To prevent unwanted fusion proteins from emerging (when this biobrick is cloned without scar), a double stop codon was inserted at the end of the promoter.
false
false
_797_
0
10153
9
It's complicated
false
The whole promoter region with all putative regulatory sequences was BioBricked. Stop codons were added in frame with the XylU coding region.
false
Michael Eichenberger
annotation2141974
1
XylU
range2141974
1
239
319
annotation2141890
1
IHF binding site
range2141890
1
130
157
annotation2141915
1
XylR binding site
range2141915
1
69
90
annotation2141889
1
PprA binding site
range2141889
1
42
61
annotation2141891
1
-24
range2141891
1
182
188
annotation2141892
1
-12
range2141892
1
193
196
annotation2141973
1
xylU start codon
range2141973
1
239
241
annotation2141983
1
double stop codon
range2141983
1
319
325
annotation2141888
1
XylR binding site
range2141888
1
37
52
BBa_K625002_sequence
1
cccgggaaagcgcgatgaaccttttttatcgctgccttgatcaaatcgacaggtggttatgcgcgattgatgatttgctcaaatacagccagcgtgctgtagattttctctcataccccccctttcttttttacaaagaaaatcaataatttagatgaaataaggggatcggtataagcaatggcatggcggttgctagctatacgagacttaaaataaaaatagtggtgacccttcaatgttgtattttctcaactctgttcagattggttgctttcgccatgtatatcctcaaagcgggccagccgtagccgttacgtaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z