LOCUS       BBa_K625004             1385 bp    DNA     linear       14-Jun-2024
DEFINITION  Minimal pBR322 pMB1 origin
ACCESSION   BBa_K625004
VERSION     BBa_K625004.1  
FEATURES             Location/Qualifiers
     misc_feature    759..1378
                     /label=pMB1 origin
     misc_feature    153..344
                     /label=ROP
     misc_feature    143..147
                     /label=ROP RBS
     misc_feature    772..1324
                     /label=RNA II transcript
     promoter        1355..1360
                     /label=RNA II promoter -35 sequence
     promoter        1334..1339
                     /label=RNA II promoter -10 sequence
     misc_feature    1214..1321
                     /label=RNA I transcript
     promoter        1178..1183
                     /label=RNA I promoter -35 sequence
     promoter        1200..1205
                     /label=RNA I promoter -10 sequence
ORIGIN
        1 cccgccgcat ccataccgcc agttgtttac cctcacaacg ttccagtaac cgggcatgtt
       61 catcatcagt aacccgtatc gtgagcatcc tctctcgttt catcggtatc attaccccca
      121 tgaacagaaa tcccccttac acggaggcat cagtgaccaa acaggaaaaa accgccctta
      181 acatggcccg ctttatcaga agccagacat taacgcttct ggagaaactc aacgagctgg
      241 acgcggatga acaggcagac atctgtgaat cgcttcacga ccacgctgat gagctttacc
      301 gcagctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
      361 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
      421 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag
      481 tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
      541 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc
      601 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
      661 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
      721 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
      781 gctccgcccc cctgacgagc atcacaaaaa tcgacgttca agtcagaggt ggcgaaaccc
      841 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
      901 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
      961 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     1021 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     1081 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     1141 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     1201 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagttg ccttcggaaa
     1261 aagagttggt agttcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     1321 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     1381 tacgg
//