LOCUS BBa_K625005 2743 bp DNA linear 30-Mar-2025 DEFINITION pSB6A5 ACCESSION BBa_K625005 VERSION BBa_K625005.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=Suffix misc_feature 2..7 /label=SpeI misc_feature 16..21 /label=PstI misc_feature 9..16 /label=NotI misc_feature 1..21 /label=BBa_G00001 misc_feature 22..26 /label=Nt.BstNB I recognition site misc_feature 27..29 /label=Arbitrary sequence (could be any bases) misc_feature 30..34 /label=Topoisomerase I recognition site misc_feature 30..30 /label=Generated T overhang misc_feature 22..34 /label=BBa_B0044 misc_feature 36..38 /label=stop codon (+2 frame) misc_feature 35..37 /label=stop codon (-1 frame) misc_feature 40..42 /label=stop codon (+3 frame) misc_feature 44..46 /label=stop codon (+1 frame) misc_feature 43..45 /label=stop codon (-2 frame) misc_feature 39..41 /label=stop codon (-3 frame) misc_feature 35..46 /label=BBa_B0042 stem_loop 57..88 /label=stem loop misc_feature 50..89 /label=identical to E. coli MG1655 between tonB and yciA terminator 47..115 /label=BBa_B0054 primer_bind 116..135 /label=VR misc_feature 116..135 /label=BBa_G00102 stem_loop 149..168 /label=stem_loop terminator 136..176 /label=BBa_B0062 misc_feature 177..183 /label=BBa_G0003 misc_feature 942..1561 /label=pMB1 origin misc_feature 336..527 /label=ROP misc_feature 326..330 /label=ROP RBS misc_feature 955..1507 /label=RNA II transcript promoter 1538..1543 /label=RNA II promoter -35 sequence promoter 1517..1522 /label=RNA II promoter -10 sequence misc_feature 1397..1504 /label=RNA I transcript promoter 1361..1366 /label=RNA I promoter -35 sequence promoter 1383..1388 /label=RNA I promoter -10 sequence misc_feature 184..1568 /label=BBa_K625004 misc_feature 1569..1576 /label=BBa_G0002 CDS 1656..2519 /label=ampicillin resistance promoter 1607..1612 /label=ampR -35 promoter 1633..1638 /label=ampR -10 RBS 1647..1651 /label=ampR RBS misc_feature 1577..2519 /label=BBa_P1002 misc_feature 2520..2526 /label=BBa_G0004 stem_loop 2535..2569 /label=stem_loop terminator 2527..2598 /label=BBa_B0053 primer_bind 2599..2618 /label=VF2 primer 2599..2618 /label=BBa_G00100 stem_loop 2654..2675 /label=stem loop from bacteriophage T3 misc_feature 2646..2684 /label=identical to bacteriophage T3 terminator 2619..2696 /label=BBa_B0055 misc_feature 2698..2700 /label=stop codon (+2 frame) misc_feature 2697..2699 /label=stop codon (-1 frame) misc_feature 2702..2704 /label=stop codon (+3 frame) misc_feature 2706..2708 /label=stop codon (+1 frame) misc_feature 2705..2707 /label=stop codon (-2 frame) misc_feature 2701..2703 /label=stop codon (-3 frame) misc_feature 2697..2708 /label=BBa_B0042 misc_feature 2709..2713 /label=Topoisomerase I recognition site misc_feature 2717..2721 /label=Nt.BstNB I recognition site misc_feature 2714..2716 /label=Arbitrary sequence (could be any bases) misc_feature 2713..2713 /label=Generated T overhang misc_feature 2709..2721 /label=BBa_B0043 misc_feature 2722..2727 /label=EcoRI site misc_feature 2737..2742 /label=XbaI site misc_feature 2722..2743 /label=Prefix misc_feature 2728..2735 /label=NotI site misc_feature 2722..2743 /label=BBa_G00000 ORIGIN 1 tactagtagc ggccgctgca ggagtcacta agggttagtt agttagatta gcagaaagtc 61 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttggggctca 121 ctcaaaggcg gtaatcagat aaaaaaaatc cttagctttc gctaaggatg atttctgcta 181 gagcccgccg catccatacc gccagttgtt taccctcaca acgttccagt aaccgggcat 241 gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt atcattaccc 301 ccatgaacag aaatccccct tacacggagg catcagtgac caaacaggaa aaaaccgccc 361 ttaacatggc ccgctttatc agaagccaga cattaacgct tctggagaaa ctcaacgagc 421 tggacgcgga tgaacaggca gacatctgtg aatcgcttca cgaccacgct gatgagcttt 481 accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc 541 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg 601 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg 661 gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat 721 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc 781 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 841 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 901 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 961 taggctccgc ccccctgacg agcatcacaa aaatcgacgt tcaagtcaga ggtggcgaaa 1021 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 1081 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 1141 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 1201 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 1261 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 1321 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 1381 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttgccttcgg 1441 aaaaagagtt ggtagttctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 1501 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 1561 ttctacggta ctagagctaa atacattcaa atatctatcc gctcatgaga caataaccct 1621 gataaatgct tcaataatat tgaaaaagga agaatatgag tattcaacat ttccgtgtcg 1681 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg 1741 tgaaagtaaa agatgccgaa gatcagttgg gtgcacgtgt gggttacatc gaactggacc 1801 tcaacagcgg taagattctt gagagttttc gccccgaaga acgtttccca atgatgagca 1861 cttttaaagt tctgctctgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac 1921 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa 1981 agcatcttac ggacggcatg acagtacgcg aattatgcag cgctgccata accatgagtg 2041 ataacacggc ggccaactta cttctgacaa cgatcggagg accgaaggag cttaccgctt 2101 ttttgcacaa catgggtgat catgtaactc gccttgatcg ttgggaaccg gagctgaatg 2161 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agctatggca acaacgttgc 2221 gcaaactctt aactggcgaa cttcttactc tcgcttcccg gcaacaatta atagactgga 2281 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta 2341 ttgctgataa atctggagcc ggtgagcgtg ggtcccgcgg tattattgca gccctggggc 2401 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagccag gcaactatgg 2461 acgaacgtaa tcgccagatc gctgagatag gtgcctccct gattaagcat tggtaataat 2521 actagctccg gcaaaaaaac gggcaaggtg tcaccaccct gccctttttc tttaaaaccg 2581 aaaagattac ttcgcgtttg ccacctgacg tctaagaaaa ggaatattca gcaatttgcc 2641 cgtgccgaag aaaggcccac ccgtgaaggt gagccagtga gttgattgct acgtaattag 2701 ttagttagcc cttagtgact cgaattcgcg gccgcttcta gag //