##gff-version 3
##sequence-region BBa_K625005 1 2743
BBa_K625005	.	sequence_feature	1569	1576	.	+	0	ID=BBa_G0002;Name=BBa_G0002
BBa_K625005	.	primer	2599	2618	.	+	0	ID=BBa_G00100;Name=BBa_G00100
BBa_K625005	.	primer_binding_site	2599	2618	.	+	0	ID=annotation1934505;Name=VF2;Parent=BBa_G00100
BBa_K625005	.	sequence_feature	2709	2721	.	+	0	ID=BBa_B0043;Name=BBa_B0043
BBa_K625005	.	sequence_feature	2709	2713	.	+	0	ID=annotation1797101;Name=Topoisomerase I recognition site;Parent=BBa_B0043
BBa_K625005	.	sequence_feature	2714	2716	.	+	0	ID=annotation1797103;Name=Arbitrary sequence (could be any bases);Parent=BBa_B0043
BBa_K625005	.	sequence_feature	2717	2721	.	+	0	ID=annotation1797102;Name=Nt.BstNB I recognition site;Parent=BBa_B0043
BBa_K625005	.	sequence_feature	2713	2713	.	+	0	ID=annotation1797104;Name=Generated T overhang;Parent=BBa_B0043
BBa_K625005	.	engineered_region	1577	2519	.	+	0	ID=BBa_P1002;Name=BBa_P1002
BBa_K625005	.	promoter	1607	1612	.	+	0	ID=annotation1897109;Name=ampR -35;Parent=BBa_P1002
BBa_K625005	.	CDS	1656	2519	.	+	0	ID=annotation1790786;Name=ampicillin resistance;Parent=BBa_P1002
BBa_K625005	.	ribosome_entry_site	1647	1651	.	+	0	ID=annotation1897111;Name=ampR RBS;Parent=BBa_P1002
BBa_K625005	.	promoter	1633	1638	.	+	0	ID=annotation1897110;Name=ampR -10;Parent=BBa_P1002
BBa_K625005	.	sequence_feature	2520	2526	.	+	0	ID=BBa_G0004;Name=BBa_G0004
BBa_K625005	.	sequence_feature	1	21	.	+	0	ID=BBa_G00001;Name=BBa_G00001
BBa_K625005	.	sequence_feature	2	7	.	+	0	ID=annotation1934226;Name=SpeI;Parent=BBa_G00001
BBa_K625005	.	engineered_region	1	21	.	+	0	ID=annotation1821544;Name=Suffix;Parent=BBa_G00001
BBa_K625005	.	sequence_feature	16	21	.	+	0	ID=annotation1934227;Name=PstI;Parent=BBa_G00001
BBa_K625005	.	sequence_feature	9	16	.	+	0	ID=annotation1934228;Name=NotI;Parent=BBa_G00001
BBa_K625005	.	sequence_feature	22	34	.	+	0	ID=BBa_B0044;Name=BBa_B0044
BBa_K625005	.	sequence_feature	30	34	.	+	0	ID=annotation1797128;Name=Topoisomerase I recognition site;Parent=BBa_B0044
BBa_K625005	.	sequence_feature	22	26	.	+	0	ID=annotation1797126;Name=Nt.BstNB I recognition site;Parent=BBa_B0044
BBa_K625005	.	sequence_feature	27	29	.	+	0	ID=annotation1797127;Name=Arbitrary sequence (could be any bases);Parent=BBa_B0044
BBa_K625005	.	sequence_feature	30	30	.	+	0	ID=annotation1797129;Name=Generated T overhang;Parent=BBa_B0044
BBa_K625005	.	terminator	47	115	.	+	0	ID=BBa_B0054;Name=BBa_B0054
BBa_K625005	.	stem_loop	57	88	.	+	0	ID=annotation1747359;Name=stem loop;Parent=BBa_B0054
BBa_K625005	.	sequence_feature	50	89	.	+	0	ID=annotation1783342;Name=identical to E. coli MG1655 between tonB and yciA;Parent=BBa_B0054
BBa_K625005	.	sequence_feature	177	183	.	+	0	ID=BBa_G0003;Name=BBa_G0003
BBa_K625005	.	sequence_feature	2722	2743	.	+	0	ID=BBa_G00000;Name=BBa_G00000
BBa_K625005	.	sequence_feature	2728	2735	.	+	0	ID=annotation1961228;Name=NotI site;Parent=BBa_G00000
BBa_K625005	.	sequence_feature	2737	2742	.	+	0	ID=annotation1934224;Name=XbaI site;Parent=BBa_G00000
BBa_K625005	.	engineered_region	2722	2743	.	+	0	ID=annotation1934225;Name=Prefix;Parent=BBa_G00000
BBa_K625005	.	sequence_feature	2722	2727	.	+	0	ID=annotation1934223;Name=EcoRI site;Parent=BBa_G00000
BBa_K625005	.	sequence_feature	35	46	.	+	0	ID=BBa_B0042;Name=BBa_B0042
BBa_K625005	.	stop_codon	35	37	.	+	0	ID=annotation1797084;Name=stop codon (-1 frame);Parent=BBa_B0042
BBa_K625005	.	stop_codon	39	41	.	+	0	ID=annotation1797088;Name=stop codon (-3 frame);Parent=BBa_B0042
BBa_K625005	.	stop_codon	43	45	.	+	0	ID=annotation1797087;Name=stop codon (-2 frame);Parent=BBa_B0042
BBa_K625005	.	stop_codon	36	38	.	+	0	ID=annotation1797083;Name=stop codon (+2 frame);Parent=BBa_B0042
BBa_K625005	.	stop_codon	40	42	.	+	0	ID=annotation1797085;Name=stop codon (+3 frame);Parent=BBa_B0042
BBa_K625005	.	stop_codon	44	46	.	+	0	ID=annotation1797086;Name=stop codon (+1 frame);Parent=BBa_B0042
BBa_K625005	.	terminator	2527	2598	.	+	0	ID=BBa_B0053;Name=BBa_B0053
BBa_K625005	.	stem_loop	2535	2569	.	+	0	ID=annotation318602;Name=stem_loop;Parent=BBa_B0053
BBa_K625005	.	terminator	136	176	.	+	0	ID=BBa_B0062;Name=BBa_B0062
BBa_K625005	.	stem_loop	149	168	.	+	0	ID=annotation318637;Name=stem_loop;Parent=BBa_B0062
BBa_K625005	.	terminator	2619	2696	.	+	0	ID=BBa_B0055;Name=BBa_B0055
BBa_K625005	.	stem_loop	2654	2675	.	+	0	ID=annotation1783301;Name=stem loop from bacteriophage T3;Parent=BBa_B0055
BBa_K625005	.	sequence_feature	2646	2684	.	+	0	ID=annotation1783311;Name=identical to bacteriophage T3;Parent=BBa_B0055
BBa_K625005	.	sequence_feature	184	1568	.	+	0	ID=BBa_K625004;Name=BBa_K625004
BBa_K625005	.	sequence_feature	942	1561	.	+	0	ID=annotation2141659;Name=pMB1 origin;Parent=BBa_K625004
BBa_K625005	.	sequence_feature	1397	1504	.	+	0	ID=annotation2141755;Name=RNA I transcript;Parent=BBa_K625004
BBa_K625005	.	sequence_feature	955	1507	.	+	0	ID=annotation2141752;Name=RNA II transcript;Parent=BBa_K625004
BBa_K625005	.	sequence_feature	336	527	.	+	0	ID=annotation2141660;Name=ROP;Parent=BBa_K625004
BBa_K625005	.	promoter	1517	1522	.	+	0	ID=annotation2141754;Name=RNA II promoter -10 sequence;Parent=BBa_K625004
BBa_K625005	.	promoter	1383	1388	.	+	0	ID=annotation2141773;Name=RNA I promoter -10 sequence;Parent=BBa_K625004
BBa_K625005	.	promoter	1538	1543	.	+	0	ID=annotation2141753;Name=RNA II promoter -35 sequence;Parent=BBa_K625004
BBa_K625005	.	promoter	1361	1366	.	+	0	ID=annotation2141772;Name=RNA I promoter -35 sequence;Parent=BBa_K625004
BBa_K625005	.	sequence_feature	326	330	.	+	0	ID=annotation2141751;Name=ROP RBS;Parent=BBa_K625004
BBa_K625005	.	sequence_feature	116	135	.	+	0	ID=BBa_G00102;Name=BBa_G00102
BBa_K625005	.	primer_binding_site	116	135	.	+	0	ID=annotation1961229;Name=VR;Parent=BBa_G00102
BBa_K625005	.	sequence_feature	2697	2708	.	+	0	ID=BBa_B0042;Name=BBa_B0042
BBa_K625005	.	stop_codon	2697	2699	.	+	0	ID=annotation1797084;Name=stop codon (-1 frame);Parent=BBa_B0042
BBa_K625005	.	stop_codon	2701	2703	.	+	0	ID=annotation1797088;Name=stop codon (-3 frame);Parent=BBa_B0042
BBa_K625005	.	stop_codon	2705	2707	.	+	0	ID=annotation1797087;Name=stop codon (-2 frame);Parent=BBa_B0042
BBa_K625005	.	stop_codon	2698	2700	.	+	0	ID=annotation1797083;Name=stop codon (+2 frame);Parent=BBa_B0042
BBa_K625005	.	stop_codon	2702	2704	.	+	0	ID=annotation1797085;Name=stop codon (+3 frame);Parent=BBa_B0042
BBa_K625005	.	stop_codon	2706	2708	.	+	0	ID=annotation1797086;Name=stop codon (+1 frame);Parent=BBa_B0042
>BBa_K625005
tactagtagcggccgctgcaggagtcactaagggttagttagttagattagcagaaagtcaaaagcctccgaccggagg
cttttgactaaaacttcccttggggttatcattggggctcactcaaaggcggtaatcagataaaaaaaatccttagctt
tcgctaaggatgatttctgctagagcccgccgcatccataccgccagttgtttaccctcacaacgttccagtaaccggg
catgttcatcatcagtaacccgtatcgtgagcatcctctctcgtttcatcggtatcattacccccatgaacagaaatcc
cccttacacggaggcatcagtgaccaaacaggaaaaaaccgcccttaacatggcccgctttatcagaagccagacatta
acgcttctggagaaactcaacgagctggacgcggatgaacaggcagacatctgtgaatcgcttcacgaccacgctgatg
agctttaccgcagctgcctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcac
agcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggcgca
gccatgacccagtcacgtagcgatagcggagtgtatactggcttaactatgcggcatcagagcagattgtactgagagt
gcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctc
actgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacag
aatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgct
ggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgttcaagtcagaggtggcgaaacccgaca
ggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggat
acctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggt
cgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtctt
gagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtag
gcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgct
gaagccagttgccttcggaaaaagagttggtagttcttgatccggcaaacaaaccaccgctggtagcggtggttttttt
gtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggtactagagctaa
atacattcaaatatctatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagaatatga
gtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgct
ggtgaaagtaaaagatgccgaagatcagttgggtgcacgtgtgggttacatcgaactggacctcaacagcggtaagatt
cttgagagttttcgccccgaagaacgtttcccaatgatgagcacttttaaagttctgctctgtggcgcggtattatccc
gtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcac
agaaaagcatcttacggacggcatgacagtacgcgaattatgcagcgctgccataaccatgagtgataacacggcggcc
aacttacttctgacaacgatcggaggaccgaaggagcttaccgcttttttgcacaacatgggtgatcatgtaactcgcc
ttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagctatggcaac
aacgttgcgcaaactcttaactggcgaacttcttactctcgcttcccggcaacaattaatagactggatggaggcggat
aaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtg
ggtcccgcggtattattgcagccctggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagcca
ggcaactatggacgaacgtaatcgccagatcgctgagataggtgcctccctgattaagcattggtaataatactagctc
cggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctg
acgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgat
tgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagag