BBa_K627000
1
mdnED
ABC transporter and N-acetyltransferase from mdn-cluster
2011-09-19T11:00:00Z
2015-05-08T01:12:56Z
The BioBrick mdnDE as a part of the microviridin gene (mdn) cluster was isolated from Microcystis aeruginosa strain NIES-843.
The BioBrick mdnED is a part of the whole microviridin gene (mdn) cluster, which encodes the protease inhibitor microviridin L. Microviridins are tricyclic depsipeptides, which are ribosomally synthesized by Microcystis (Ziemert et al., 2010). They have a promising potential for therapy as they can block disease-relevant proteases (Ziemert et al., 2008).
Microviridins are synthesized from a ribosomal precursor peptide (MdnA). Additionally, the microviridin L biosynthesis gene cluster consists of genes encoding an ATP-grasp-type ligase (mdnB and mdnC) and genes, which encode an ABC transporter (mdnE) and a N-acetyltransferase of the GNAT family (mdnD) (Ziemert et al., 2008).
In the following BioBrick mdnED the genes mdnD (N-acetyltransferase of the GNAT family) and mdnE (ABC transporter) is encoded (Ziemert et al., 2008).
Because this BioBrick is an expression part, the adenin of mdnE gene's start codon is part of the XbaI recognition site.
false
false
_799_
0
10585
9
In stock
false
This biobrick was built by PCR using the following PCR primers
Forward primer: TAAATGAATTCGCGGCCGCTTCTAGATGCCTCAATATACTACTAAAC
Reverse primer: ATTTCTGCAGCGGCCGCTACTAGTATCAGCAAACCCTACTTAATTTC
To insert mdnED in the vector pSb1C3, the resulting PCR product and the vector were digested with the restriction enzymes, EcoRI and SpeI.
false
iGEM2011 Potsdam_Bioware
annotation2135038
1
stop codon
range2135038
1
2061
2063
annotation2135039
1
start codon
range2135039
1
2210
2212
annotation2135036
1
mdnE
range2135036
1
1
2063
annotation2135041
1
start codon
range2135041
1
1
2
annotation2135040
1
stop codon
range2135040
1
2741
2743
annotation2135037
1
mdnD
range2135037
1
2210
2743
BBa_K627000_sequence
1
tgcctcaatatactactaaacaagcaacggaaaaccctgttgtgtccgaccagtccgccaagaatcccttttcactatttggtcaattttgggaaagcctaaaaattgtcgctcaaccctattggtatccaacggagttaaatggacgtgcctttggagatgtgatcatttcttgggggatgtcagctttgattcttttaagtatcttggcaacggtgggggtagaagcttttagtagctactggaatcgttatgtactcgacattattattgaagatagagatctttctaaatatctaaatacgctgtggttatccagttcgttgattatattaacaacggggttatttgctttttctcaatttattcgtcgcaaagtagccttagattggtataagtggttaacgaaacaaaccgtcaaaaaataactcaattatcgcgcttattataacattgattttacctcagctttaaaaaatcccgatcaaagattatctcaagaaattgaaccaattacgaccatgaccctaaggttgttaattaccttcgtggaaaaagggttacaaatgattacttttgcgattattctttggacaatttctcggcaaattgcggtttatctaataatttatacgatcgcaggaaatttcatagcaatttatctaactcaagaactaaacaagattaatcaatctgagcttaataataaagctgattataattatgctttaacccatgtgcgtaaccacgccgaatccattgctttttttcagggagaagagcaggaagaaaaaatcattgaaggccgttttgaacgggttattcaaagctcggaaaaactgataaattgggaacggtttaataatctttttaatcgaggttatcagtcagcgattagtgtattttcaatgtttattttgaccccgatgtttattaaaaatgaaatcgattatggggaaataagtcaagcgagtttatgctgtttccttttttctagtgccttgggacaattaattactgaatggggaacatcgggtaaagtttcaagttatattgaacggttagctaccttgacagatgccttaaaagttgccagtgaagagccgaaaaacttaagtcgtattatcaccctggaagataatcgtctagcttttgaaaattttaccctacaaactcccggttatgaaaaggtaattgtggagaatttatcggtttctgtccctttgggagaaagcctactgattattggccccagtggtcgaggaaaaagttccttgttacgagcaatcgccggtttatggaaagcaggtagcggccgttcagtgcgtcctcctttagctaaaatgttatttttgccccaacgtccttatattatccttggcagtttacgccagcaattactttatccccataccaatgatcaggtgagcgatgaaagattagaaaatatcttaaaaaaagtcaacctgcaacatttattaactgacaaaaatagcttagataaagaagtcaactgggaacaaattttatctctgggagaacaacaaagattagcttttgctcgactattaatcactaaacctacctttactatcttagatgaagcaactagcgctttagatttggccaatgaagccagcttatatcagcaattacaggaaagtgaaaccacttttattagtgttggtcatcgggaaagtttatttaattatcatcgatgggtcttagagttaacagaaaattcccattggcagctgccaactgtagaagattatcagcgcaaaaaagtgaataacctagtgatgatgtcaaaaaaatcagaaaatttttcggcggtaaaaatagagactttgtctgaggttaaaactgagcatgatgcggaaattgcaggagtttctgaaatagccgaaggtttgtcccatcaacaaatgcagtccctaactgattattctttaagtagcgttcgtaatcgagccagtttaggtaaaatcattactgccaaagatggttttaattatcgttacgataaagacccaaaaaccttaaaatgggtgagaatatagctaatggggtgacaaacagattgaaagtgagacagagtattcagacttccccctctcaggagaactagagaatcaggtttttgctgggtaagttgattaacaagctactaactgataactgataactggtataggagttttatctcatgaaagcactggaaaaactggtgacggaacgattatatttagtccccttcaagcttgaacttctcaaggccgcaattataggtatcgatgaattagcaacaattttgggggtaaaagttgcagctgactgggccgaacaagagttgattcaagctttccctgatattgcgaatattttgctgcaattccccttacaaaatgagtgggggtgggggagtttaattattcatcaaacagaaaatacccttattggtcatgtgatgatcaaaattattcctgatgtcacaggtttacccacagatgctgtagaaattggctatcaagtagcgccgttatatcgacgacaaggctacgcatctgaggcgacaaaagcaatgatggactggacactttctcaacctggtatgcaaacagtgaccgctggctgcgctcccgataatatagcttcaaagcgagttttagaaaaaataggcatggatctggtagaagcacgagagaaagtcttggtctggaaattaagtagggtttgctgat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z