BBa_K627004
1
mdnD
N-acetyltransferase from the mdn-cluster
2011-09-20T11:00:00Z
2015-05-08T01:12:56Z
The BioBrick mdnD as a part of the microviridin gene (mdn) cluster was isolated from Microcystis aeruginosa strain NIES-843.
The BioBrick mdnD is a part of the whole microviridin gene (mdn) cluster, which encodes the protease
inhibitor microviridin L. Microviridins are tricyclic depsipeptides, which are ribosomally synthesized
by Microcystis (Ziemert et al., 2010). They have a promising potential for therapy as they can block
disease-relevant proteases (Ziemert et al., 2008).
<br><br>
Microviridins are synthesized from a ribosomal precursor peptide (MdnA). Additionally, the microviridin L
biosynthesis gene cluster consists of genes encoding an ATP-grasp-type ligase (mdnB and mdnC) and genes,
which encode an ABC transporter (mdnE) and a N-acetyltransferase of the GNAT family (mdnD) (Ziemert et al.,
2008).
<br><br>
The following BioBrick mdnD encodes a N-acetyltransferase of the GNAT family (Ziemert et al., 2008).
<br><br>
Because this BioBrick is a RF10 expression part the adenine of mdnD gene's start codon is part of the XbaI
recognition site.
false
false
_799_
0
10585
9
In stock
false
This BioBrick was built by PCR using the following PCR primers<br>
Forward primer: TATATGAATTCGCGGCCGCTTCTAGATGAAAGCACTGGAAAAACTG<br>
Reverse primer: ATTTCTGCAGCGGCCGCTACTAGTATCAGCAAACCCTACTTAATTTC <br>
To insert mdnD in the vector pSB1C3, the resulting PCR product and the vector were digested with the restriction enzymes EcoRI and SpeI.
<br><br>
Because this BioBrick is a RF10 expression part the adenine of mdnD gene start codon is part of the XbaI recognition site.
false
iGEM11 Potsdam_Bioware
annotation2136264
1
mdnD
range2136264
1
1
533
annotation2136266
1
stop
range2136266
1
531
533
annotation2136265
1
start
range2136265
1
1
2
BBa_K627004_sequence
1
tgaaagcactggaaaaactggtgacggaacgattatatttagtccccttcaagcttgaacttctcaaggccgcaattataggtatcgatgaattagcaacaattttgggggtaaaagttgcagctgactgggccgaacaagagttgattcaagctttccctgatattgcgaatattttgctgcaattccccttacaaaatgagtgggggtgggggagtttaattattcatcaaacagaaaatacccttattggtcatgtgatgatcaaaattattcctgatgtcacaggtttacccacagatgctgtagaaattggctatcaagtagcgccgttatatcgacgacaaggctacgcatctgaggcgacaaaagcaatgatggactggacactttctcaacctggtatgcaaacagtgaccgctggctgcgctcccgataatatagcttcaaagcgagttttagaaaaaataggcatggatctggtagaagcacgagagaaagtcttggtctggaaattaagtagggtttgctgat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z