LOCUS BBa_K628001 10663 bp DNA linear 16-Feb-2025 DEFINITION antimicrobial peptide kill switch ACCESSION BBa_K628001 VERSION BBa_K628001.1 FEATURES Location/Qualifiers promoter 1..131 /label=promoter misc_feature 40..57 /label=AraI1 misc_feature 61..78 /label=AraI2 promoter 1..130 /label=BBa_K206000 misc_feature 139..5704 /label=BBa_J61011 CDS 6529..7202 /label=TRP1 misc_feature 5976..6431 /label=F1_Ori, M13_Ori primer_bind 7549..7566 /label=M13F CDS 8582..9241 /label=AmpR misc_feature 5713..5733 /label=Biobrick suffix misc_feature 9339..10321 /label=ColE1_Origin misc_feature 10505..10526 /label=Biobrick prefix misc_feature 7734..8252 /label=CEN6_ARS4 misc_feature 5713..10526 /label=BBa_K268000 stem_loop 10546..10589 /label=stem_loop misc_feature 10535..10614 /label=BBa_B0010 terminator 10535..10614 /label=BBa_B0010 stem_loop 10630..10649 /label=T7 TE misc_feature 10656..10656 /label=stop polyA_site 10650..10663 /label=polya misc_feature 10623..10663 /label=BBa_B0012 terminator 10623..10663 /label=BBa_B0012 terminator 10535..10663 /label=BBa_B0015 ORIGIN 1 acattgatta tttgcacggc gtcacacttt gctatgccat agcaagatag tccataagat 61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 121 ttgggctagc tactagaggc ggccgcatta cgctgacttg acgggacggc ggctttgtta 181 ctagtcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 241 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 301 atttatcagc aataaaccag ccagccggaa gggccgatcc cgcgaaatta atacgactca 361 ctatagggag accacaacgg tttccctcta gacctaacta actaaagatt aactttataa 421 ggaggaaaaa catatgagta aaggagaaga acttttcact ggagttgtcc caattcttgt 481 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga 541 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc 601 atggccaaca cttgtcacta ctttgactta tggtgttcaa tgcttttcaa gatacccaga 661 tcatatgaaa cggcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag 721 aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg 781 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat 841 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa 901 acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt 961 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc 1021 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga 1081 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact 1141 atacaaaagg cctgcagcaa acgacgaaaa ctacgcttta gtagcttaat aagcttaatt 1201 agctgagctt ggactcctgt tgatagatcc agtaatgacc tcagaactcc atctggattt 1261 gttcagaacg ctcggttgcc gccgggcgtt ttttattggt gagaatccaa gctagcttgg 1321 cgagattttc aggagctaag gatcaattcg aagcttgggc ccgaacaaaa actcatctca 1381 gaagaggatc tgaatagcgc cgtcgaccat catcatcatc atcattgagt ttaaacggtc 1441 tccagcttgg ctgttttggc ggatgagaga agattttcag cctgatacag attaaatcag 1501 aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac 1561 ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc 1621 cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac 1681 tgggcctttc gttttatctg ttgtttgtcg gtgaactaat tcttgaagac gaaagggcct 1741 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg 1801 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc 1861 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag 1921 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg 1981 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt 2041 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt 2101 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt 2161 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa 2221 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag 2281 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac 2341 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac 2401 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac 2461 cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac 2521 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact 2581 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg 2641 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt 2701 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat 2761 aggtgcctca ctgattaagc attggtaacc cgggaccaag tttactcata tatactttag 2821 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat 2881 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa 2941 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca 3001 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt 3061 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg 3121 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc 3181 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga 3241 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc 3301 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc 3361 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca 3421 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg 3481 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta 3541 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct 3601 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag 3661 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa 3721 gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc 3781 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc 3841 cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg 3901 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg 3961 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt 4021 aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca 4081 gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa 4141 gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg 4201 gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac 4261 atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc 4321 agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac 4381 agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact 4441 tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg 4501 tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct 4561 gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca 4621 tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga 4681 tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca 4741 agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc 4801 cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag 4861 gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata 4921 aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga 4981 tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc 5041 gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg 5101 cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc 5161 ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg 5221 caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc 5281 ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa 5341 gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg 5401 gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa 5461 gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa 5521 ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca 5581 agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata 5641 ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag 5701 gatctactag agtactagta gcggccgctg cagcccgggg gatccaatag ttctagtgcg 5761 gccgccaccg cggtggagct ccaattcgcc ctatagtgag tcgtattaca attcactggc 5821 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc 5881 agcacatccc cccttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc 5941 ccaacagttg cgcagcctga atggcgaatg gcgcgacgcg ccctgtagcg gcgcattaag 6001 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc 6061 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc 6121 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa 6181 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg 6241 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac 6301 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta 6361 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac 6421 gtttacaatt tcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc 6481 aggcaagtgc acaaacaata cttaaataaa tactactcag taataaccta tttcttagca 6541 tttttgacga aatttgctat tttgttagag tcttttacac catttgtctc cacacctccg 6601 cttacatcaa caccaataac gccatttaat ctaagcgcat caccaacatt ttctggcgtc 6661 agtccaccag ctaacataaa atgtaagctt tcggggctct cttgccttcc aacccagtca 6721 gaaatcgagt tccaatccaa aagttcacct gtcccacctg cttctgaatc aaacaaggga 6781 ataaacgaat gaggtttctg tgaagctgca ctgagtagta tgttgcagtc ttttggaaat 6841 acgagtcttt taataactgg caaaccgagg aactcttggt attcttgcca cgactcatct 6901 ccatgcagtt ggacgatatc aatgccgtaa tcattgacca gagccaaaac atcctcctta 6961 ggttgattac gaaacacgcc aaccaagtat ttcggagtgc ctgaactatt tttatatgct 7021 tttacaagac ttgaaatttt ccttgcaata accgggtcaa ttgttctctt tctattgggc 7081 acacatataa tacccagcaa gtcagcatcg gaatctagtg cacattctgc ggcctctgtg 7141 ctctgcaagc cgcaaacttt caccaatgga ccagaactac ctgtgaaatt aataacagac 7201 atactccaag ctgcctttgt gtgcttaatc acgtatactc acgtgctcaa tagtcaccaa 7261 tgccctccct cttggccctc tccttttctt ttttcgaccg aattaattct taatcggcaa 7321 aaaaagaaaa gctccggatc aagattgtac gtaaggtgac aagctatttt tcaataaaga 7381 atatcttcca ctactgccat ctggcgtcat aactgcaaag tacacatata ttacgatgct 7441 gtctattaaa tgcttcctat attatatata tagtaatgtc gtttatggtg cactctcagt 7501 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac 7561 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc 7621 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc 7681 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttaggacgga 7741 tcgcttgcct gtaacttaca cgcgcctcgt atcttttaat gatggaataa tttgggaatt 7801 tactctgtgt ttatttattt ttatgttttg tatttggatt ttagaaagta aataaagaag 7861 gtagaagagt tacggaatga agaaaaaaaa ataaacaaag gtttaaaaaa tttcaacaaa 7921 aagcgtactt tacatatata tttattagac aagaaaagca gattaaatag atatacattc 7981 gattaacgat aagtaaaatg taaaatcaca ggattttcgt gtgtggtctt ctacacagac 8041 aagatgaaac aattcggcat taatacctga gagcaggaag agcaagataa aaggtagtat 8101 ttgttggcga tccccctaga gtcttttaca tcttcggaaa acaaaaacta ttttttcttt 8161 aatttctttt tttactttct atttttaatt tatatattta tattaaaaaa tttaaattat 8221 aattattttt atagcacgtg atgaaaagga cccaggtggc acttttcggg gaaatgtgcg 8281 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca 8341 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt 8401 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga 8461 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga 8521 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat 8581 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca 8641 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt 8701 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac 8761 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct 8821 aaccgctttt tttcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga 8881 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac 8941 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat 9001 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg 9061 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc 9121 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg gcagtcaggc 9181 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg 9241 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta 9301 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg 9361 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga 9421 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt 9481 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag 9541 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa 9601 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag 9661 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca 9721 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac 9781 cgaactgaga tacctacagc gtgagcattg agaaagcgcc acgcttcccg aagggagaaa 9841 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc 9901 aggggggaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg 9961 tcgatttttg tgatgctcgt caggggggcc gagcctatgg aaaaacgcca gcaacgcggc 10021 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc 10081 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag 10141 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa 10201 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga 10261 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tacctcactc attaggcacc 10321 ccaggcttta cactttatgc ttccggctcc tatgttgtgt ggaattgtga gcggataaca 10381 atttcacaca ggaaacagct atgaccatga ttacgccaag ctcggaatta accctcacta 10441 aagggaacaa aagctgggta ccgggccccc cctcgaggtc gacggtatcg ataagcttga 10501 tatcgaattc gcggccgctt ctagagtact agagccaggc atcaaataaa acgaaaggct 10561 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 10621 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata //