BBa_K628001
type: ComponentDefinition
persistent identity: BBa K628001
display id: BBa_K628001
version: 1
was generated by: iGEM to SBOL conversion
description: antimicrobial peptide kill switch
created: 2011-09-18T11:00:00Z
modified: 2015-05-08T01:12:56Z
mutable provenance: It comes from pigs. Again, I will update soon.
owned by: james
owned by: myers
mutable description: This will be updated soon, I promise.
discontinued: true
dominant: false
experience: experience/None
group u list: _800_
m user id: 0
owner id: 9738
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: TBA
star: false
creator: Charles Thompson
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K206000
component: BBa_B0015
component: BBa_K268000
component: BBa_J61011
sequence annotation: BBa_K206000
sequence annotation: BBa_J61011
sequence annotation: BBa_K268000
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K628001_sequence/1

BBa_K206000
type: Component
persistent identity: component2134003
display id: component2134003
version: 1
top level: BBa_K628001
definition: pBAD
access: public

BBa_B0015
type: Component
persistent identity: component2134020
display id: component2134020
version: 1
top level: BBa_K628001
definition: BBa_B0015
access: public

BBa_K268000
type: Component
persistent identity: component2134013
display id: component2134013
version: 1
top level: BBa_K628001
definition: BBa_K268000
access: public

BBa_J61011
type: Component
persistent identity: component2134004
display id: component2134004
version: 1
top level: BBa_K628001
definition: BBa_J61011
access: public

BBa_K206000
type: SequenceAnnotation
persistent identity: annotation2134003
display id: annotation2134003
version: 1
top level: BBa_K628001
location: https://synbiohub.org/public/igem/BBa_K628001/annotation2134003/range2134003/1
component: BBa_K206000

https://synbiohub.org/public/igem/BBa_K628001/annotation2134003/range2134003/1
type: Range
persistent identity: range2134003
display id: range2134003
version: 1
top level: BBa_K628001
direction: direction/reverse
start: 1
end: 130
orientation: inline

BBa_J61011
type: SequenceAnnotation
persistent identity: annotation2134004
display id: annotation2134004
version: 1
top level: BBa_K628001
location: https://synbiohub.org/public/igem/BBa_K628001/annotation2134004/range2134004/1
component: BBa_J61011

https://synbiohub.org/public/igem/BBa_K628001/annotation2134004/range2134004/1
type: Range
persistent identity: range2134004
display id: range2134004
version: 1
top level: BBa_K628001
direction: direction/reverse
start: 139
end: 5704
orientation: inline

BBa_K268000
type: SequenceAnnotation
persistent identity: annotation2134013
display id: annotation2134013
version: 1
top level: BBa_K628001
location: https://synbiohub.org/public/igem/BBa_K628001/annotation2134013/range2134013/1
component: BBa_K268000

https://synbiohub.org/public/igem/BBa_K628001/annotation2134013/range2134013/1
type: Range
persistent identity: range2134013
display id: range2134013
version: 1
top level: BBa_K628001
direction: direction/reverse
start: 5713
end: 10526
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2134020
display id: annotation2134020
version: 1
top level: BBa_K628001
location: https://synbiohub.org/public/igem/BBa_K628001/annotation2134020/range2134020/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K628001/annotation2134020/range2134020/1
type: Range
persistent identity: range2134020
display id: range2134020
version: 1
top level: BBa_K628001
direction: direction/reverse
start: 10535
end: 10663
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_J61011
type: ComponentDefinition
persistent identity: BBa J61011
display id: BBa_J61011
version: 1
was generated by: iGEM to SBOL conversion
description: pBR939b
created: 2006-08-06T11:00:00Z
modified: 2015-08-31T02:02:59Z
mutable provenance: later
owned by: james
owned by: myers
mutable description: later
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: N/A
star: false
creator: John Anderson
type: DnaRegion
role: partType/Plasmid
role: SO:0000155
sequence: https://synbiohub.org/public/igem/BBa_J61011_sequence/1

pBAD
type: ComponentDefinition
persistent identity: BBa K206000
display id: BBa_K206000
version: 1
was generated by: iGEM to SBOL conversion
description: pBAD strong
created: 2009-10-13T11:00:00Z
modified: 2015-05-08T01:11:23Z
mutable provenance: The sequence was obtained by applying all mutations that upregulated AraC binding and subsequent promoter activity listed in reference [1].
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: pBAD is an E.coli promoter that is induced by L-arabinose. K206000 is a mutagenized pBAD promoter that is responsive to lower concentrations of arabinose than wild type (<partinfo>I13453</partinfo>) and, additionally, exhibits a higher maximum of protein expression as measured by coupling to a fluorescent reporter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _307_
m user id: 0
owner id: 4172
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: There were no special design considerations.
star: false
creator: Amelia Hardjasa
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence annotation: AraI1
sequence annotation: AraI2
sequence: https://synbiohub.org/public/igem/BBa_K206000_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2049252
display id: annotation2049252
version: 1
top level: pBAD
location: https://synbiohub.org/public/igem/BBa_K206000/annotation2049252/range2049252/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K206000/annotation2049252/range2049252/1
type: Range
persistent identity: range2049252
display id: range2049252
version: 1
top level: pBAD
direction: direction/forward
start: 1
end: 131
orientation: inline

AraI1
type: SequenceAnnotation
persistent identity: annotation2049253
display id: annotation2049253
version: 1
top level: pBAD
location: https://synbiohub.org/public/igem/BBa_K206000/annotation2049253/range2049253/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K206000/annotation2049253/range2049253/1
type: Range
persistent identity: range2049253
display id: range2049253
version: 1
top level: pBAD
direction: direction/forward
start: 40
end: 57
orientation: inline

AraI2
type: SequenceAnnotation
persistent identity: annotation2049254
display id: annotation2049254
version: 1
top level: pBAD
location: https://synbiohub.org/public/igem/BBa_K206000/annotation2049254/range2049254/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K206000/annotation2049254/range2049254/1
type: Range
persistent identity: range2049254
display id: range2049254
version: 1
top level: pBAD
direction: direction/forward
start: 61
end: 78
orientation: inline

BBa_K268000
type: ComponentDefinition
persistent identity: BBa K268000
display id: BBa_K268000
version: 1
was generated by: iGEM to SBOL conversion
description: pSB6A0
created: 2009-10-20T11:00:00Z
modified: 2015-05-08T01:11:43Z
mutable provenance: Unmodified version can be found by accession number U03440.
owned by: james
owned by: myers
mutable description: Biobricked plasmid backbone pRS314 with XbaI, PstI and SpeI sites removed from outside the MCS. One of a series of pBluescript based yeast centrometer vectors developed by Sikorski R.S., Hieter P (Genetics 122:19-27(1989)). Confers Trp resistence in yeast and ampicillan resistance in E. coli where it can be transformed and amplified for cloning purposes.
discontinued: false
dominant: false
experience: experience/None
group u list: _379_
m user id: 0
owner id: 2483
owning group id: 170
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Removal of non-biobrick compatible restriction sites.
star: false
creator: Nora Yucel
type: DnaRegion
role: partType/Plasmid Backbone
role: SO:0000755
sequence annotation: Biobrick suffix
sequence annotation: F1_Ori, M13_Ori
sequence annotation: TRP1
sequence annotation: M13F
sequence annotation: CEN6_ARS4
sequence annotation: AmpR
sequence annotation: ColE1_Origin
sequence annotation: Biobrick prefix
sequence: https://synbiohub.org/public/igem/BBa_K268000_sequence/1

Biobrick suffix
type: SequenceAnnotation
persistent identity: annotation2062715
display id: annotation2062715
version: 1
top level: BBa_K268000
location: https://synbiohub.org/public/igem/BBa_K268000/annotation2062715/range2062715/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K268000/annotation2062715/range2062715/1
type: Range
persistent identity: range2062715
display id: range2062715
version: 1
top level: BBa_K268000
direction: direction/forward
start: 1
end: 21
orientation: inline

F1_Ori, M13_Ori
type: SequenceAnnotation
persistent identity: annotation2062712
display id: annotation2062712
version: 1
top level: BBa_K268000
location: https://synbiohub.org/public/igem/BBa_K268000/annotation2062712/range2062712/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K268000/annotation2062712/range2062712/1
type: Range
persistent identity: range2062712
display id: range2062712
version: 1
top level: BBa_K268000
direction: direction/forward
start: 264
end: 719
orientation: inline

TRP1
type: SequenceAnnotation
persistent identity: annotation2062711
display id: annotation2062711
version: 1
top level: BBa_K268000
location: https://synbiohub.org/public/igem/BBa_K268000/annotation2062711/range2062711/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K268000/annotation2062711/range2062711/1
type: Range
persistent identity: range2062711
display id: range2062711
version: 1
top level: BBa_K268000
direction: direction/reverse
start: 817
end: 1490
orientation: inline

M13F
type: SequenceAnnotation
persistent identity: annotation2062713
display id: annotation2062713
version: 1
top level: BBa_K268000
location: https://synbiohub.org/public/igem/BBa_K268000/annotation2062713/range2062713/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_K268000/annotation2062713/range2062713/1
type: Range
persistent identity: range2062713
display id: range2062713
version: 1
top level: BBa_K268000
direction: direction/forward
start: 1837
end: 1854
orientation: inline

CEN6_ARS4
type: SequenceAnnotation
persistent identity: annotation2062718
display id: annotation2062718
version: 1
top level: BBa_K268000
location: https://synbiohub.org/public/igem/BBa_K268000/annotation2062718/range2062718/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K268000/annotation2062718/range2062718/1
type: Range
persistent identity: range2062718
display id: range2062718
version: 1
top level: BBa_K268000
direction: direction/forward
start: 2022
end: 2540
orientation: inline

AmpR
type: SequenceAnnotation
persistent identity: annotation2062714
display id: annotation2062714
version: 1
top level: BBa_K268000
location: https://synbiohub.org/public/igem/BBa_K268000/annotation2062714/range2062714/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K268000/annotation2062714/range2062714/1
type: Range
persistent identity: range2062714
display id: range2062714
version: 1
top level: BBa_K268000
direction: direction/forward
start: 2870
end: 3529
orientation: inline

ColE1_Origin
type: SequenceAnnotation
persistent identity: annotation2062716
display id: annotation2062716
version: 1
top level: BBa_K268000
location: https://synbiohub.org/public/igem/BBa_K268000/annotation2062716/range2062716/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K268000/annotation2062716/range2062716/1
type: Range
persistent identity: range2062716
display id: range2062716
version: 1
top level: BBa_K268000
direction: direction/forward
start: 3627
end: 4609
orientation: inline

Biobrick prefix
type: SequenceAnnotation
persistent identity: annotation2062717
display id: annotation2062717
version: 1
top level: BBa_K268000
location: https://synbiohub.org/public/igem/BBa_K268000/annotation2062717/range2062717/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K268000/annotation2062717/range2062717/1
type: Range
persistent identity: range2062717
display id: range2062717
version: 1
top level: BBa_K268000
direction: direction/forward
start: 4793
end: 4814
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K628001_sequence/1
type: Sequence
persistent identity: BBa K628001 sequence
display id: BBa_K628001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagaggcggccgcattacgctgacttgacgggacggcggctttgttactagtcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgatcccgcgaaattaatacgactcactatagggagaccacaacggtttccctctagacctaactaactaaagattaactttataaggaggaaaaacatatgagtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttgacttatggtgttcaatgcttttcaagatacccagatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgcagcaaacgacgaaaactacgctttagtagcttaataagcttaattagctgagcttggactcctgttgatagatccagtaatgacctcagaactccatctggatttgttcagaacgctcggttgccgccgggcgttttttattggtgagaatccaagctagcttggcgagattttcaggagctaaggatcaattcgaagcttgggcccgaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcatcatcatcattgagtttaaacggtctccagcttggctgttttggcggatgagagaagattttcagcctgatacagattaaatcagaacgcagaagcggtctgataaaacagaatttgcctggcggcagtagcgcggtggtcccacctgaccccatgccgaactcagaagtgaaacgccgtagcgccgatggtagtgtggggtctccccatgcgagagtagggaactgccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaactaattcttgaagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61011_sequence/1
type: Sequence
persistent identity: BBa J61011 sequence
display id: BBa_J61011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcggccgcattacgctgacttgacgggacggcggctttgttactagtcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgatcccgcgaaattaatacgactcactatagggagaccacaacggtttccctctagacctaactaactaaagattaactttataaggaggaaaaacatatgagtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttgacttatggtgttcaatgcttttcaagatacccagatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgcagcaaacgacgaaaactacgctttagtagcttaataagcttaattagctgagcttggactcctgttgatagatccagtaatgacctcagaactccatctggatttgttcagaacgctcggttgccgccgggcgttttttattggtgagaatccaagctagcttggcgagattttcaggagctaaggatcaattcgaagcttgggcccgaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcatcatcatcattgagtttaaacggtctccagcttggctgttttggcggatgagagaagattttcagcctgatacagattaaatcagaacgcagaagcggtctgataaaacagaatttgcctggcggcagtagcgcggtggtcccacctgaccccatgccgaactcagaagtgaaacgccgtagcgccgatggtagtgtggggtctccccatgcgagagtagggaactgccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaactaattcttgaagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcactttta…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K206000_sequence/1
type: Sequence
persistent identity: BBa K206000 sequence
display id: BBa_K206000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K268000_sequence/1
type: Sequence
persistent identity: BBa K268000 sequence
display id: BBa_K268000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcagcccgggggatccaatagttctagtgcggccgccaccgcggtggagctccaattcgccctatagtgagtcgtattacaattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccccttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcgacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcaggcaagtgcacaaacaatacttaaataaatactactcagtaataacctatttcttagcatttttgacgaaatttgctattttgttagagtcttttacaccatttgtctccacacctccgcttacatcaacaccaataacgccatttaatctaagcgcatcaccaacattttctggcgtcagtccaccagctaacataaaatgtaagctttcggggctctcttgccttccaacccagtcagaaatcgagttccaatccaaaagttcacctgtcccacctgcttctgaatcaaacaagggaataaacgaatgaggtttctgtgaagctgcactgagtagtatgttgcagtcttttggaaatacgagtcttttaataactggcaaaccgaggaactcttggtattcttgccacgactcatctccatgcagttggacgatatcaatgccgtaatcattgaccagagccaaaacatcctccttaggttgattacgaaacacgccaaccaagtatttcggagtgcctgaactatttttatatgcttttacaagacttgaaattttccttgcaataaccgggtcaattgttctctttctattgggcacacatataatacccagcaagtcagcatcggaatctagtgcacattctgcggcctctgtgctctgcaagccgcaaactttcaccaatggaccagaactacctgtgaaattaataacagacatactccaagctgcctttgtgtgcttaatcacgtatactcacgtgctcaatagtcaccaatgccctccctcttggccctctccttttcttttttcgaccgaattaattcttaatcggcaaaaaaagaaaagctccggatcaagattgtacgtaaggtgacaagctatttttcaataaagaatatcttccactactgccatctggcgtcataactgcaaagtacacatatattacgatgctgtctattaaatgcttcctatattatatatatagtaatgtcgtttatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z