It comes from pigs. Again, I will update soon.
Double terminator consisting of BBa_B0010 and BBa_B0012
The sequence was obtained by applying all mutations that upregulated AraC binding and subsequent promoter activity listed in reference [1].
pBAD is an E.coli promoter that is induced by L-arabinose. K206000 is a mutagenized pBAD promoter that is responsive to lower concentrations of arabinose than wild type (<partinfo>I13453</partinfo>) and, additionally, exhibits a higher maximum of protein expression as measured by coupling to a fluorescent reporter.
There were no special design considerations.
Unmodified version can be found by accession number U03440.
Biobricked plasmid backbone pRS314 with XbaI, PstI and SpeI sites removed from outside the MCS. One of a series of pBluescript based yeast centrometer vectors developed by Sikorski R.S., Hieter P (Genetics 122:19-27(1989)). Confers Trp resistence in yeast and ampicillan resistance in E. coli where it can be transformed and amplified for cloning purposes.
Removal of non-biobrick compatible restriction sites.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
Conversion of the iGEM parts registry to SBOL2.1