BBa_K309012 1 BBa_K309012 Unc-54 3' UTR (for use in C. elegans chassis) 2010-10-21T11:00:00Z 2015-05-08T01:11:53Z From C. elegans genomic DNA. This is a terminator for use in C. elegans chassis. false false _428_ 0 4458 9 It's complicated false The exact length and sequence of the functional sequence that acts as the promoter in C. elegans has not been elucidated. Thus this sequence is a section of genomic DNA that exists in behind of the Unc-54 gene that has been previously demonstrated to function as a promoter, with the same expression pattern as the Unc-54 gene. false Chris Palmer BBa_K093005 1 RBS-RFP RFP with RBS 2008-10-27T12:00:00Z 2015-05-08T01:08:40Z registry plasmids Released HQ 2013 RFP, BBa_E1010, with Elowitz RBS, BBa_B0034. false false _247_ 0 3630 9 In stock false The part was needed for downstream applications. There can be no expression of a reporter without a ribosome binding site. true Kathy Lam, Danielle Nash component2244025 1 BBa_E1010 component2244022 1 BBa_B0034 annotation2244022 1 BBa_B0034 range2244022 1 1 12 annotation2244025 1 BBa_E1010 range2244025 1 19 724 BBa_E1010 1 mRFP1 **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral) 2004-07-27T11:00:00Z 2015-08-31T04:07:26Z Campbell et al., PNAS v99 p7877 <a href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12060735">URL</a> Released HQ 2013 monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm false false _11_1_ 0 52 7 In stock false TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A. true Drew Endy annotation1014044 1 mrfp1 range1014044 1 1 675 annotation2214014 1 Help:Barcodes range2214014 1 682 706 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K631002 1 BBa_K631002 let-858 (promoter; for use in C. elegans) 2011-09-13T11:00:00Z 2015-05-08T01:12:56Z test test false false _805_ 0 9721 9 It's complicated false test false Allister Smith BBa_K631040 1 BBa_K631040 let-858:RFP:UNC-54 2011-09-27T11:00:00Z 2015-05-08T01:12:57Z Test Test false false _805_ 0 9721 9 It's complicated false Test false Allister Smith component2250755 1 BBa_K093005 component2250756 1 BBa_K309012 component2250749 1 BBa_K631002 annotation2250755 1 BBa_K093005 range2250755 1 2009 2732 annotation2250756 1 BBa_K309012 range2250756 1 2741 3632 annotation2250749 1 BBa_K631002 range2250749 1 1 2000 BBa_K093005_sequence 1 aaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc BBa_K309012_sequence 1 cattcgtagatttccaactgagcgccggtcgctaccattaccaacttgtctggtgtcaaaaataataggggccgctgtcatcagagtaagtttaaactgagttctactaactaacgagtaatatttaaattttcagcatctcgcgcccgtgcctctgacttctaagtccaattactcttcaacatccctacatgctctttctccctgtgctcccaccccctatttttgttattatcaaaaaaacttcttcttaatttctttgttttttagcttcttttaagtcacctctaacaatgaaattgtgtagattcaaaaatagaattaattcgtaataaaaagtcgaaaaaaattgtgctccctccccccattaataataattctatcccaaaatctacacaatgttctgtgtacacttcttatgttttttttacttctgataaattttttttgaaacatcatagaaaaaaccgcacacaaaataccttatcatatgttacgtttcagtttatgaccgcaatttttatttcttcgcacgtctgggcctctcatgacgtcaaatcatgctcatcgtgaaaaagttttggagtatttttggaatttttcaatcaagtgaaagtttatgaaattaattttcctgcttttgctttttgggggtttcccctattgtttgtcaagagtttcgaggacggcgtttttcttgctaaaatcacaagtattgatgagcacgatgcaagaaagatcggaagaaggtttgggtttgaggctcagtggaaggtgagtagaagttgataatttgaaagtggagtagtgtctatggggtttttgccttaaatgacagaatacattcccaatataccaaacataactgtttcctacttgtcggccgtacggg BBa_B0034_sequence 1 aaagaggagaaa BBa_K631040_sequence 1 tttattaattcaaataaaagttacaacgtttaaaaccaacgagatgggctttttttaattcaagcttcggccctataaatcaacaagacccataaattgtgatgtataaatcttcaatcttaaatctcataatcattgatatgatcaatcaaaactttaatatataattaaagaaacacctctatggattacatcaatgtttatccaacaaattgcctaatccaattcctctctgaacacctggtttagcatctctccactgccgaaaatgcatggaaacaagcgtcatatcagatatccgataggttgcttcatttcagttttcttctctctttgcttcgtgtttcttataaacaaaaagaaacacaaatctttgatgaatgaaaattcagacatcgttggaggggatggttgaccttcaggcggctcaattcattaccagtttcacaaaaaattcgatgattatctctataaattgtcaagagaaaatgaaaaaaagaaaaccttttcgctttctgccgagccatgacaagatgcctatcttttactattttctctctgtcactttcggtaaacccgccaaggacggacacgatttcatgcttcggttgtaaacaaattcagtgtgaataagaagaatattcggaggaaaggcgacggaaattatcaaatttacagccagacatcatgtgagacaagagaactatatgaatatgcaaaataaattgaattggattgtttagttggccagttggaagggagtcatagaatgaacacacaaattcagttatcaaaaaagaagtctagcttgagttcttcacgaattatgatcctagaactattcaggaattatgatcctagaactattcaggaattatttcagaaatcggaaaactaaataaccggtaaaatgttcacaaaacccttttggaattataataagttttccaaaaaaagattgataattttacattcaaaatcacacactttcgcttttgaggcaggttttgagcagcttttcaaaactttaaattggaagtgaagttagtttcaagaagtaaaaatatttctctatacttttatctaaatttccaatcaaaaaactttgaaaaacgatgaaaaaatggagattttgtaagcgagccgaaaaaaagttcagcacatatctgttacctgccatatactgaacatgacaaaatatatcagaacaaaattaagttttatgcatagtagggatgtttttgcatattttatcactgaaaaatcttttaatatcagtagtttcagaaaagttacatgccattttctgaaaatttcaatttttgctcataaaatcctccattttcaaaattttcgcttcaaattttgattaaacatgttatttttgagtcttctattagattctgaaagaattttttaaaaatgttggctcatctccaaaatcgtcttccgctctgaaaaacgaaagtggacctttgacatccgaaaaaatgggcgaaaaaatgaaattgagctttttgggtcgaaaaaaatgtttttagaatgctgagaacacgttaaacacgaagatcatatttattttgagacccggatgctctgaaaatgtctgacatagatttaaaaaagcatatatatatttttcattttcaacgtgaaagttttgtgcaactttatagaatctcctattggcacattgttttttatttaactgaggcagtttttgaacacctttttgaaactttgaatctctttgaagtatactgtcgaaaagactgacttgagcgttcgaaatgccagaagaaaactatatttgaatctcgcgctaaattgagaaatgcaaccgcgctccactggacaattggaaaaaaaatttattcggaggcgacaacggtattttcgaaattgattttctgtgtattttctcattttttataaattcttctttgatttatcgttcgtttgtgagaaatttaattgtattcaaacttttttatagtaagtactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagcattcgtagatttccaactgagcgccggtcgctaccattaccaacttgtctggtgtcaaaaataataggggccgctgtcatcagagtaagtttaaactgagttctactaactaacgagtaatatttaaattttcagcatctcgcgcccgtgcctctgacttctaagtccaattactcttcaacatccctacatgctctttctccctgtgctcccaccccctatttttgttattatcaaaaaaacttcttcttaatttctttgttttttagcttcttttaagtcacctctaacaatgaaattgtgtagattcaaaaatagaattaattcgtaataaaaagtcgaaaaaaattgtgctccctccccccattaataataattctatcccaaaatctacacaatgttctgtgtacacttcttatgttttttttacttctgataaattttttttgaaacatcatagaaaaaaccgcacacaaaataccttatcatatgttacgtttcagtttatgaccgcaatttttatttcttcgcacgtctgggcctctcatgacgtcaaatcatgctcatcgtgaaaaagttttggagtatttttggaatttttcaatcaagtgaaagtttatgaaattaattttcctgcttttgctttttgggggtttcccctattgtttgtcaagagtttcgaggacggcgtttttcttgctaaaatcacaagtattgatgagcacgatgcaagaaagatcggaagaaggtttgggtttgaggctcagtggaaggtgagtagaagttgataatttgaaagtggagtagtgtctatggggtttttgccttaaatgacagaatacattcccaatataccaaacataactgtttcctacttgtcggccgtacggg BBa_E1010_sequence 1 atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc BBa_K631002_sequence 1 tttattaattcaaataaaagttacaacgtttaaaaccaacgagatgggctttttttaattcaagcttcggccctataaatcaacaagacccataaattgtgatgtataaatcttcaatcttaaatctcataatcattgatatgatcaatcaaaactttaatatataattaaagaaacacctctatggattacatcaatgtttatccaacaaattgcctaatccaattcctctctgaacacctggtttagcatctctccactgccgaaaatgcatggaaacaagcgtcatatcagatatccgataggttgcttcatttcagttttcttctctctttgcttcgtgtttcttataaacaaaaagaaacacaaatctttgatgaatgaaaattcagacatcgttggaggggatggttgaccttcaggcggctcaattcattaccagtttcacaaaaaattcgatgattatctctataaattgtcaagagaaaatgaaaaaaagaaaaccttttcgctttctgccgagccatgacaagatgcctatcttttactattttctctctgtcactttcggtaaacccgccaaggacggacacgatttcatgcttcggttgtaaacaaattcagtgtgaataagaagaatattcggaggaaaggcgacggaaattatcaaatttacagccagacatcatgtgagacaagagaactatatgaatatgcaaaataaattgaattggattgtttagttggccagttggaagggagtcatagaatgaacacacaaattcagttatcaaaaaagaagtctagcttgagttcttcacgaattatgatcctagaactattcaggaattatgatcctagaactattcaggaattatttcagaaatcggaaaactaaataaccggtaaaatgttcacaaaacccttttggaattataataagttttccaaaaaaagattgataattttacattcaaaatcacacactttcgcttttgaggcaggttttgagcagcttttcaaaactttaaattggaagtgaagttagtttcaagaagtaaaaatatttctctatacttttatctaaatttccaatcaaaaaactttgaaaaacgatgaaaaaatggagattttgtaagcgagccgaaaaaaagttcagcacatatctgttacctgccatatactgaacatgacaaaatatatcagaacaaaattaagttttatgcatagtagggatgtttttgcatattttatcactgaaaaatcttttaatatcagtagtttcagaaaagttacatgccattttctgaaaatttcaatttttgctcataaaatcctccattttcaaaattttcgcttcaaattttgattaaacatgttatttttgagtcttctattagattctgaaagaattttttaaaaatgttggctcatctccaaaatcgtcttccgctctgaaaaacgaaagtggacctttgacatccgaaaaaatgggcgaaaaaatgaaattgagctttttgggtcgaaaaaaatgtttttagaatgctgagaacacgttaaacacgaagatcatatttattttgagacccggatgctctgaaaatgtctgacatagatttaaaaaagcatatatatatttttcattttcaacgtgaaagttttgtgcaactttatagaatctcctattggcacattgttttttatttaactgaggcagtttttgaacacctttttgaaactttgaatctctttgaagtatactgtcgaaaagactgacttgagcgttcgaaatgccagaagaaaactatatttgaatctcgcgctaaattgagaaatgcaaccgcgctccactggacaattggaaaaaaaatttattcggaggcgacaacggtattttcgaaattgattttctgtgtattttctcattttttataaattcttctttgatttatcgttcgtttgtgagaaatttaattgtattcaaacttttttatagtaag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z