BBa_K633014 1 BBa_K633014 AraC+Pbad promoter+RBS+signal peptide phoA+Cellulase+Linker+estA membrane protein 2011-09-26T11:00:00Z 2015-06-19T01:50:43Z asdasda The composite part includes a functional expression vector for autotransporter membrane protein estA, with a linker united to a cellulase, attached to a membrane signaling peptide. This was our final construct missing a terminator false false _807_ 4206 9146 9 It's complicated true asdasdadd false Ricardo Camilo Chavez Martinez component2159635 1 BBa_K206000 component2159631 1 BBa_I13458 component2159639 1 BBa_K633002 component2159642 1 BBa_K633001 annotation2159631 1 BBa_I13458 range2159631 1 1 1114 annotation2159639 1 BBa_K633002 range2159639 1 1261 3173 annotation2159635 1 BBa_K206000 range2159635 1 1123 1252 annotation2159642 1 BBa_K633001 range2159642 1 3182 4057 BBa_K633001 1 BBa_K633001 Linker + EstA Membrane Protein 2011-09-26T11:00:00Z 2015-05-08T01:12:57Z where does it come from? We used a linker attached to a protein membrane "estA" false false _807_ 0 9146 9 It's complicated false None whatsoever false Ricardo Camilo Chavez Martinez annotation2145003 1 estA range2145003 1 34 876 annotation2145004 1 linker range2145004 1 1 33 BBa_K633002 1 BBa_K633002 RBS+signal peptide phoA+Cellulase 2011-09-26T11:00:00Z 2015-06-19T01:49:52Z Source We used Rybosome Binding Site BBa_B0034 designed by by Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr, evaluated by Warsaw's 2010 team. we added membrane protein signal peptide phoA and cellulase immediately next to it false false _807_ 4206 9146 9 It's complicated false Since this part is supposed to be attached to part BBa_K633001 in order to geerate part BBa_K633014, we had to begin with both the signal peptide and then the extracelular domain, which is located on the N terminal side of the polypeptide chain. false Ricardo Camilo Chavez Martinez annotation2145250 1 Cellulase range2145250 1 78 1913 annotation2145137 1 part BBa_B0034 range2145137 1 1 14 annotation2145138 1 Signal Peptide range2145138 1 15 77 BBa_I13458 1 BBa_I13458 promoter C and arabinose C operon 2005-08-16T11:00:00Z 2015-08-31T04:07:34Z Released HQ 2013 PC and AraC from the AraBAD operator. PC and AraC are on the complementary strand, reading right to left. false false _11_ 0 253 6 In stock false true Josh Michener BBa_K206000 1 pBAD pBAD strong 2009-10-13T11:00:00Z 2015-05-08T01:11:23Z The sequence was obtained by applying all mutations that upregulated AraC binding and subsequent promoter activity listed in reference [1]. Released HQ 2013 pBAD is an <i>E.coli</i> promoter that is induced by L-arabinose. K206000 is a mutagenized pBAD promoter that is responsive to lower concentrations of arabinose than wild type (<partinfo>I13453</partinfo>) and, additionally, exhibits a higher maximum of protein expression as measured by coupling to a fluorescent reporter. false false _307_ 0 4172 9 In stock true There were no special design considerations. false Amelia Hardjasa annotation2049254 1 AraI2 range2049254 1 61 78 annotation2049252 1 promoter range2049252 1 1 131 annotation2049253 1 AraI1 range2049253 1 40 57 BBa_K633002_sequence 1 aggaggtaaaacatatgaaacaatcaaccatcgcactggcgctgctgccgctgctgttcaccccggtcaccaaagccgagctcgcaaaaatcacggaaaattatcagtttgattcacgtattcgcctgaactcgattggcttcatcccgaatcatagtaaaaaagcgacgattgcggccaactgctccaccttttatgtggttaaagaagatggcaccatcgtgtacaccggtacggcgaccagcatgtttgataacgacacgaaagaaaccgtctatattgccgatttcagctctgtgaatgaagaaggtacctattacctggctgtcccgggtgtgggcaaatcagttaacttcaaaatcgcgatgaacgtctatgaagatgcgttcaaaaccgccatgctgggcatgtacctgctgcgttgcggcacgtcagtgtcggccacctataatggcattcattactctcacggtccgtgtcataccaacgatgcatatctggactacatcaatggccagcatacgaaaaaagatagtaccaaaggctggcacgacgcgggtgattataacaaatacgtcgtgaatgccggcattaccgttggttccatgtttctggcatgggaacacttcaaagatcagctggaaccggtcgccctggaaattccggagaaaaacaattctatcccggactttctggatgaactgaaatacgaaatcgattggatcctgacgatgcagtacccggacggtagcggccgtgttgcgcataaagtctctacccgcaactttggcggtttcatcatgccggaaaatgaacacgatgaacgtttctttgtcccgtggagttccgcagctacggcagattttgtggcgatgaccgccatggcggcccgtattttccgcccgtatgatccgcagtacgcggaaaaatgcatcaacgcagctaaagtgagctacgaatttctgaaaaacaatccggcaaacgtttttgctaatcaaagcggcttctctacgggtgaatacgcaaccgtgtctgatgctgatgaccgtctgtgggcggcggcggaaatgtgggaaaccctgggtgatgaagaatatctgcgtgactttgaaaaccgcgctgcgcagttcagcaagaaaattgaagcagattttgactgggataacgttgctaatctgggcatgttcacctatctgctgagcgaacgtccgggtaaaaatccggccctggtccagtctattaaagatagtctgctgtccacggcagacagcatcgtgcgtacctctcaaaaccatggttatggccgcaccctgggcaccacgtattactggggctgtaatggtacggttgtccgccagaccatgattctgcaagtggcaaacaaaatcagcccgaacaatgattatgtgaatgccgcactggacgcgatttcacatgtttttggtcgtaactattacaatcgctcgtacgttaccggtctgggcattaacccgccgatgaatccgcacgatcgtcgctctggtgcagacggtatctgggaaccgtggccgggttatctggttggcggtggctggccgggtccgaaagactgggtcgatattcaggactcataccaaaccaacgaaattgcgatcaactggaatgctgcgctgatctatgctctggcgggcttcgtgaactacaattcgccgcagaatgaagtcctgtatggcgatgtgaacgatgacggtaaagttaattcgacggacctgaccctgctgaaacgttacgtgctgaaagccgttagcacgctgccgtcatcgaaagcggagaaaaacgccgatgtcaatcgtgacggtcgcgttaacagctctgatgtcaccattctgagccgttatctgattcgcgttatcgaaaaactgccgatcgctagc BBa_K633014_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctattactagagacattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaggaggtaaaacatatgaaacaatcaaccatcgcactggcgctgctgccgctgctgttcaccccggtcaccaaagccgagctcgcaaaaatcacggaaaattatcagtttgattcacgtattcgcctgaactcgattggcttcatcccgaatcatagtaaaaaagcgacgattgcggccaactgctccaccttttatgtggttaaagaagatggcaccatcgtgtacaccggtacggcgaccagcatgtttgataacgacacgaaagaaaccgtctatattgccgatttcagctctgtgaatgaagaaggtacctattacctggctgtcccgggtgtgggcaaatcagttaacttcaaaatcgcgatgaacgtctatgaagatgcgttcaaaaccgccatgctgggcatgtacctgctgcgttgcggcacgtcagtgtcggccacctataatggcattcattactctcacggtccgtgtcataccaacgatgcatatctggactacatcaatggccagcatacgaaaaaagatagtaccaaaggctggcacgacgcgggtgattataacaaatacgtcgtgaatgccggcattaccgttggttccatgtttctggcatgggaacacttcaaagatcagctggaaccggtcgccctggaaattccggagaaaaacaattctatcccggactttctggatgaactgaaatacgaaatcgattggatcctgacgatgcagtacccggacggtagcggccgtgttgcgcataaagtctctacccgcaactttggcggtttcatcatgccggaaaatgaacacgatgaacgtttctttgtcccgtggagttccgcagctacggcagattttgtggcgatgaccgccatggcggcccgtattttccgcccgtatgatccgcagtacgcggaaaaatgcatcaacgcagctaaagtgagctacgaatttctgaaaaacaatccggcaaacgtttttgctaatcaaagcggcttctctacgggtgaatacgcaaccgtgtctgatgctgatgaccgtctgtgggcggcggcggaaatgtgggaaaccctgggtgatgaagaatatctgcgtgactttgaaaaccgcgctgcgcagttcagcaagaaaattgaagcagattttgactgggataacgttgctaatctgggcatgttcacctatctgctgagcgaacgtccgggtaaaaatccggccctggtccagtctattaaagatagtctgctgtccacggcagacagcatcgtgcgtacctctcaaaaccatggttatggccgcaccctgggcaccacgtattactggggctgtaatggtacggttgtccgccagaccatgattctgcaagtggcaaacaaaatcagcccgaacaatgattatgtgaatgccgcactggacgcgatttcacatgtttttggtcgtaactattacaatcgctcgtacgttaccggtctgggcattaacccgccgatgaatccgcacgatcgtcgctctggtgcagacggtatctgggaaccgtggccgggttatctggttggcggtggctggccgggtccgaaagactgggtcgatattcaggactcataccaaaccaacgaaattgcgatcaactggaatgctgcgctgatctatgctctggcgggcttcgtgaactacaattcgccgcagaatgaagtcctgtatggcgatgtgaacgatgacggtaaagttaattcgacggacctgaccctgctgaaacgttacgtgctgaaagccgttagcacgctgccgtcatcgaaagcggagaaaaacgccgatgtcaatcgtgacggtcgcgttaacagctctgatgtcaccattctgagccgttatctgattcgcgttatcgaaaaactgccgatcgctagctactagagggtggcggttcaggcggtggctcgggtggcggtcagaatgtgggccaatggcgtggttttgttggcggtggcggtcagcgcctggacttcgattcacaagattcggccgcaagcggtgacggcaacggttataatctgaccctgggcggtagttaccgtattgatgaagcatggcgtgctggtgtggctgcgggtttttatcgtcagaaactggaagccggcgcaaaagactccgattatcgcatgaacagctacatggcttctgcgttcgttcagtaccaagaaaatcgttggtgggcagatgcagcactgacgggcggttatctggactacgatgacctgaaacgtaaatttgccctgggcggtggcgaacgcagtgaaaaaggcgataccaatggtcatctgtgggcgttctccgcccgtctgggttatgatatcgcacagcaagctgactcaccgtggcacctgagtccgtttgtgtccgcggattatgcccgcgtggaagttgacggctacagtgaaaaaggtgcctccgcaacggctctggattatgatgaccagaaacgtagttccaaacgcctgggcgcgggtctgcaaggcaaatacgcctttggtagtgatacccaactgttcgcagaatatgctcatgaacgtgaatacgaagatgacacgcaggatctgaccatgtctctgaacagtctgccgggcaatcgttttacgctggaaggttataccccgcaggatcacctgaaccgcgtgtcactgggcttctcgcaaaaactggcgccggaactgagcctgcgcggtggctataattggcgcaaaggcgaagatgatacgcaacagtccgtttctctggctctgagtctggatttc BBa_I13458_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctat BBa_K633001_sequence 1 ggtggcggttcaggcggtggctcgggtggcggtcagaatgtgggccaatggcgtggttttgttggcggtggcggtcagcgcctggacttcgattcacaagattcggccgcaagcggtgacggcaacggttataatctgaccctgggcggtagttaccgtattgatgaagcatggcgtgctggtgtggctgcgggtttttatcgtcagaaactggaagccggcgcaaaagactccgattatcgcatgaacagctacatggcttctgcgttcgttcagtaccaagaaaatcgttggtgggcagatgcagcactgacgggcggttatctggactacgatgacctgaaacgtaaatttgccctgggcggtggcgaacgcagtgaaaaaggcgataccaatggtcatctgtgggcgttctccgcccgtctgggttatgatatcgcacagcaagctgactcaccgtggcacctgagtccgtttgtgtccgcggattatgcccgcgtggaagttgacggctacagtgaaaaaggtgcctccgcaacggctctggattatgatgaccagaaacgtagttccaaacgcctgggcgcgggtctgcaaggcaaatacgcctttggtagtgatacccaactgttcgcagaatatgctcatgaacgtgaatacgaagatgacacgcaggatctgaccatgtctctgaacagtctgccgggcaatcgttttacgctggaaggttataccccgcaggatcacctgaaccgcgtgtcactgggcttctcgcaaaaactggcgccggaactgagcctgcgcggtggctataattggcgcaaaggcgaagatgatacgcaacagtccgtttctctggctctgagtctggatttc BBa_K206000_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z