LOCUS       BBa_K633015             2992 bp    DNA     linear       09-May-2024
DEFINITION  AraC+Pbad promoter+RBS+ Ompa&lpp signal peptide+Sacc
ACCESSION   BBa_K633015
VERSION     BBa_K633015.1  
FEATURES             Location/Qualifiers
     misc_feature    1..1114
                     /label=BBa_I13458
     promoter        1123..1253
                     /label=promoter
     misc_feature    1162..1179
                     /label=AraI1
     misc_feature    1183..1200
                     /label=AraI2
     promoter        1123..1252
                     /label=BBa_K206000
     misc_feature    1265..1268
                     /label=conserved
     RBS             1261..1272
                     /label=BBa_B0034
     misc_feature    1281..1287
                     /label=NdeI
     CDS             1284..1718
                     /label=OmpA
     CDS             1719..1744
                     /label=linker
     misc_feature    1739..1744
                     /label=SacI
     misc_feature    1284..1286
                     /label=ATG
     misc_feature    1281..1744
                     /label=BBa_K103006
     CDS             1751..2992
                     /label=Sacc
     CDS             1751..2992
                     /label=BBa_K633003
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctattactag agacattgat tatttgcacg
     1141 gcgtcacact ttgctatgcc atagcaagat agtccataag attagcggat cctacctgac
     1201 gctttttatc gcaactctct actgtttctc cataccgttt ttttgggcta gctactagag
     1261 aaagaggaga aatactagag catatgaaag ctactaaact ggtactgggc gcggtaatcc
     1321 tgggttctac tctgctggca ggttgctcca gcaacgctaa aatcgatcag ggaattaacc
     1381 cgtatgttgg ctttgaaatg ggttacgact ggttaggtcg tatgccgtac aaaggcagcg
     1441 ttgaaaacgg tgcatacaaa gctcagggcg ttcaactgac cgctaaactg ggttacccaa
     1501 tcactgacga cctggacatc tacactcgtc tgggtggcat ggtatggcgt gcagacacta
     1561 aatccaacgt ttatggtaaa aaccacgaca ccggcgtttc tccggtcttc gctggcggtg
     1621 ttgagtacgc gatcactcct gaaatcgcta cccgtctgga ataccagtgg accaacaaca
     1681 tcggtgacgc acacaccatc ggcactcgtc cggacaacgg cggaggttct ggaggaggga
     1741 gctctactag atgtttaatt ttaatgccag tcgctggacg cgagcccaag cgatgaaagt
     1801 gaataaattt gatttgacga cctctatgcc ggaaatcggc actgattttc ccattatgcg
     1861 tgatgacttg tggctgtggg atacttggcc attacgggat atcaatggca atcctgtcag
     1921 ctttaaaggc tggaatgtta ttttctcttt ggtcgctgac cgcaatattc cgtggaatga
     1981 tcgccattct catgcccgca tcggctattt ctattccaaa gatggtaaaa gctgggttta
     2041 tggtggccat ctattgcaag aatcggccaa tacccgcacg gcagaatggt ccggcggcac
     2101 gattatggca ccgggttccc gtaatcaggt cgaaaccttc tttacctcga ctttattcga
     2161 caagaatggc gtcagagaag ccgttgctgc tgttacgaaa ggccgcattt atgcggatag
     2221 tgaaggcgtt tggttcaagg gttttgacca gtcaacggat ttgtttcagg ctgatggtct
     2281 gttttatcaa aattatgcag aaaataatct ctggaatttc cgtgatcctc atgttttcat
     2341 caatcctgag gatggtgaga cctatgcttt gttcgaagcc aatgttgcca ctgtccgtgg
     2401 agaagacgat ataggcgagg atgaaattgg tcctgttccg gccaatacgg tcgttccaaa
     2461 agatgccaat ttatgttcgg cttctattgg tatcgcgcgt tgtttgtcgc cggatcgcac
     2521 cgaatgggag ctattaccgc ctttgttgac agcctttggc gtcaatgacc agatggaacg
     2581 gccccatgtc attttccaga atggtttgac ctatctcttt acgatcagcc atgattcgac
     2641 ttatgccgat ggcttaaccg gttccgatgg tctttatggc tttgtttccg aaaatggtat
     2701 ttttggccct tatgagccgc tgaatggttc tggccttgtt ctcggtggcc ctgcgtcaca
     2761 gccgaccgaa gcctatgctc attacatcat gaataatggc ttggttgaat cttttatcaa
     2821 tgaaatcatt gatcccaaaa gcggcaaagt tattgcgggc ggtagcttgg caccgacggt
     2881 tcgcgttgaa ttacagggac atgagacttt cgcgaccgaa gtctttgatt atggatatat
     2941 tcccgcatct tatgcttggc cggtatggcc tttccctgat cgtcgcaaat aa
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