BBa_K633003 1 BBa_K633003 Extracellular Succrase Sacc 2011-09-26T11:00:00Z 2015-05-08T01:12:57Z Source* Sacc Extracellular Sucrase false false _807_ 0 9146 9 It's complicated false None Whatsoever false Ricardo Camilo Chavez Martinez annotation2145141 1 Sacc range2145141 1 1 1242 BBa_K206000 1 pBAD pBAD strong 2009-10-13T11:00:00Z 2015-05-08T01:11:23Z The sequence was obtained by applying all mutations that upregulated AraC binding and subsequent promoter activity listed in reference [1]. Released HQ 2013 pBAD is an <i>E.coli</i> promoter that is induced by L-arabinose. K206000 is a mutagenized pBAD promoter that is responsive to lower concentrations of arabinose than wild type (<partinfo>I13453</partinfo>) and, additionally, exhibits a higher maximum of protein expression as measured by coupling to a fluorescent reporter. false false _307_ 0 4172 9 In stock true There were no special design considerations. false Amelia Hardjasa annotation2049252 1 promoter range2049252 1 1 131 annotation2049253 1 AraI1 range2049253 1 40 57 annotation2049254 1 AraI2 range2049254 1 61 78 BBa_K633015 1 BBa_K633015 AraC+Pbad promoter+RBS+ Ompa&lpp signal peptide+Sacc 2011-09-27T11:00:00Z 2015-05-08T01:12:57Z Description Description false false _807_ 0 9146 9 It's complicated true Description false Ricardo Camilo Chavez Martinez component2145199 1 BBa_K206000 component2145195 1 BBa_I13458 component2145207 1 BBa_K103006 component2145201 1 BBa_B0034 component2145209 1 BBa_K633003 annotation2145199 1 BBa_K206000 range2145199 1 1123 1252 annotation2145201 1 BBa_B0034 range2145201 1 1261 1272 annotation2145207 1 BBa_K103006 range2145207 1 1281 1744 annotation2145209 1 BBa_K633003 range2145209 1 1751 2992 annotation2145195 1 BBa_I13458 range2145195 1 1 1114 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_I13458 1 BBa_I13458 promoter C and arabinose C operon 2005-08-16T11:00:00Z 2015-08-31T04:07:34Z Released HQ 2013 PC and AraC from the AraBAD operator. PC and AraC are on the complementary strand, reading right to left. false false _11_ 0 253 6 In stock false true Josh Michener BBa_K103006 1 OmpA OmpA outer membrane protein A fused to linker; displays proteins on cell surface 2008-09-13T11:00:00Z 2015-05-08T01:08:46Z One of our basic bricks used to create fusions attached to outer membrane false true _180_ 0 2582 9 In stock true true Michael Lower annotation1990000 1 OmpA range1990000 1 4 438 annotation1990002 1 SacI range1990002 1 459 464 annotation1989999 1 NdeI range1989999 1 1 7 annotation1990001 1 linker range1990001 1 439 464 annotation1993480 1 ATG range1993480 1 4 6 BBa_K103006_sequence 1 catatgaaagctactaaactggtactgggcgcggtaatcctgggttctactctgctggcaggttgctccagcaacgctaaaatcgatcagggaattaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtccggacaacggcggaggttctggaggagggagctc BBa_B0034_sequence 1 aaagaggagaaa BBa_I13458_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctat BBa_K633003_sequence 1 atgtttaattttaatgccagtcgctggacgcgagcccaagcgatgaaagtgaataaatttgatttgacgacctctatgccggaaatcggcactgattttcccattatgcgtgatgacttgtggctgtgggatacttggccattacgggatatcaatggcaatcctgtcagctttaaaggctggaatgttattttctctttggtcgctgaccgcaatattccgtggaatgatcgccattctcatgcccgcatcggctatttctattccaaagatggtaaaagctgggtttatggtggccatctattgcaagaatcggccaatacccgcacggcagaatggtccggcggcacgattatggcaccgggttcccgtaatcaggtcgaaaccttctttacctcgactttattcgacaagaatggcgtcagagaagccgttgctgctgttacgaaaggccgcatttatgcggatagtgaaggcgtttggttcaagggttttgaccagtcaacggatttgtttcaggctgatggtctgttttatcaaaattatgcagaaaataatctctggaatttccgtgatcctcatgttttcatcaatcctgaggatggtgagacctatgctttgttcgaagccaatgttgccactgtccgtggagaagacgatataggcgaggatgaaattggtcctgttccggccaatacggtcgttccaaaagatgccaatttatgttcggcttctattggtatcgcgcgttgtttgtcgccggatcgcaccgaatgggagctattaccgcctttgttgacagcctttggcgtcaatgaccagatggaacggccccatgtcattttccagaatggtttgacctatctctttacgatcagccatgattcgacttatgccgatggcttaaccggttccgatggtctttatggctttgtttccgaaaatggtatttttggcccttatgagccgctgaatggttctggccttgttctcggtggccctgcgtcacagccgaccgaagcctatgctcattacatcatgaataatggcttggttgaatcttttatcaatgaaatcattgatcccaaaagcggcaaagttattgcgggcggtagcttggcaccgacggttcgcgttgaattacagggacatgagactttcgcgaccgaagtctttgattatggatatattcccgcatcttatgcttggccggtatggcctttccctgatcgtcgcaaataa BBa_K206000_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc BBa_K633015_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctattactagagacattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagagcatatgaaagctactaaactggtactgggcgcggtaatcctgggttctactctgctggcaggttgctccagcaacgctaaaatcgatcagggaattaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtccggacaacggcggaggttctggaggagggagctctactagatgtttaattttaatgccagtcgctggacgcgagcccaagcgatgaaagtgaataaatttgatttgacgacctctatgccggaaatcggcactgattttcccattatgcgtgatgacttgtggctgtgggatacttggccattacgggatatcaatggcaatcctgtcagctttaaaggctggaatgttattttctctttggtcgctgaccgcaatattccgtggaatgatcgccattctcatgcccgcatcggctatttctattccaaagatggtaaaagctgggtttatggtggccatctattgcaagaatcggccaatacccgcacggcagaatggtccggcggcacgattatggcaccgggttcccgtaatcaggtcgaaaccttctttacctcgactttattcgacaagaatggcgtcagagaagccgttgctgctgttacgaaaggccgcatttatgcggatagtgaaggcgtttggttcaagggttttgaccagtcaacggatttgtttcaggctgatggtctgttttatcaaaattatgcagaaaataatctctggaatttccgtgatcctcatgttttcatcaatcctgaggatggtgagacctatgctttgttcgaagccaatgttgccactgtccgtggagaagacgatataggcgaggatgaaattggtcctgttccggccaatacggtcgttccaaaagatgccaatttatgttcggcttctattggtatcgcgcgttgtttgtcgccggatcgcaccgaatgggagctattaccgcctttgttgacagcctttggcgtcaatgaccagatggaacggccccatgtcattttccagaatggtttgacctatctctttacgatcagccatgattcgacttatgccgatggcttaaccggttccgatggtctttatggctttgtttccgaaaatggtatttttggcccttatgagccgctgaatggttctggccttgttctcggtggccctgcgtcacagccgaccgaagcctatgctcattacatcatgaataatggcttggttgaatcttttatcaatgaaatcattgatcccaaaagcggcaaagttattgcgggcggtagcttggcaccgacggttcgcgttgaattacagggacatgagactttcgcgaccgaagtctttgattatggatatattcccgcatcttatgcttggccggtatggcctttccctgatcgtcgcaaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z