BBa_K634002 1 BBa_K634002 exaDE 2011-09-15T11:00:00Z 2015-05-08T01:12:57Z Cloning of k634000 and k634001. The 2 component system that responds to ethanol that activates transcription of pexaA. false false _809_ 0 9393 9 Not in stock false Each part contains a RBS but the part does not contain a promoter. false Kenneth O. Xu component2129709 1 BBa_K634000 component2129712 1 BBa_K634001 annotation2129712 1 BBa_K634001 range2129712 1 850 1541 annotation2129709 1 BBa_K634000 range2129709 1 1 841 BBa_K634000 1 BBa_K634000 exaD 2011-09-11T11:00:00Z 2015-05-08T01:12:57Z De novo synthesis, codon optimized for E. coli. Original amino acid sequence taken from Pseudomonas aeruginosa. ExaD phosphorylates ExaE, which is a transcription factor acting upon PexaA. false false _809_ 0 9290 9 It's complicated false The synthesis included a Shine-Dalgarno-complimentary RBS 5' of the gene. false Eric Walters annotation2127310 1 RBS range2127310 1 9 22 annotation2127311 1 exaD range2127311 1 23 841 BBa_K634001 1 BBa_K634001 exaE 2011-09-15T11:00:00Z 2015-05-08T01:12:57Z de novo synthesis original amino acid sequence from pseudomonas aeruginosa. Released HQ 2013 exaE is a transcription factor that is activated by phosphorylation from exaD. It acts upon pexaA. false false _809_ 0 9393 9 In stock false contains no biobrick cutsites false Kenneth O. Xu annotation2129706 1 exaE range2129706 1 15 692 annotation2129705 1 rbs range2129705 1 1 14 BBa_K634001_sequence 1 aggaggaaataaaaatgaaaattctgctggttgatgatcattttgtggttcgtgaaggtctggcagcactgctgcgtggtctgctgccggatatggaagttaatgaagccggtgatggtgaagaagcactgcaagcagttcagcgtgaaattccgagcctggttattgttgatctgggtctgcctggtattagcggtctggaactgacccgtcgtctgcgtcagcgtctgccgcaactgcgtgttctgttttttagcctgcatgatgaactggcactggttcgtcaggcactggatgccggtgcacgtggttatgttaccaaacgtgcagcaccgaccgttctgctggaagcaattcgtcgtgttctggcaggtcaactgtatctggaacagccgctggcaacccgtctggcatgtcagagctgggaagaacagggtggtgcagcactgcgtggcctgacccgtcgcgaatttgaaatttttcgtctgctggcacgtggtctggcactgcgtgaagttgcagaacatctgggtgttagcgcaaaaaccgttagcaatcatgttagcctgctgaaacagaaactgcaagttagcacccaggcagaactggttcatcgtgccattgatagcggtgttctgcgcctgggactgccgctgccgctggcagaagaagcacgcggtagcctgggttaa BBa_K634000_sequence 1 gtactagaaggaggaaataaaaatggatcgtagccgtcgtcatgcacatcgtcgtcatccgggtgttgcacatccggcagatccgcagcctgcacgtgttcgttttccgctgtttccgcctccgcgtccgggtaaaacctgtcgtctgcgtcgtcgtaccggtctgagtgatcgtccgctgagccatgcaatggatgatctgcgtccggctggtccggcagaatatcgttggctgctggcagttcaggaacgtgaacgtcgtctgctggcacaggcagttcatgatgatattggtcagtatctgaccggtattcgtacccagttactgctgctgcgttgtacagccggtgatggtcagcctgcactggcacgtctggaacgttattgtcagggtctggatgatagctttcgtgcactgctgcgtggtctgcctccgcctgcactgcaacgtcagagcctggatgaagcactgcgtgcatgtgttgcagattggcaggaaggtcagggtattgcctgtgaactgcgtctgggtggtccgctgcctccgctggatgcagcagcaggcacccatctgtatcgtctgctgcaagaagcactgaataacgttgcacgtcatgccggtgcaagccgtgttcgtattcgtctgcaatgtcgtggtggttttctgtatctgagcgttcgtgatgatggtcgtggttgtgcagcagatccggcaccgggttttggtctggatagtatgcgtcagcgtgcacgttgtctgggtgcagaactgagcctgcgtagccgtccgggtcagggcctggcactgcgtctgcgtatgcctctggaacgtgatgattaa BBa_K634002_sequence 1 gtactagaaggaggaaataaaaatggatcgtagccgtcgtcatgcacatcgtcgtcatccgggtgttgcacatccggcagatccgcagcctgcacgtgttcgttttccgctgtttccgcctccgcgtccgggtaaaacctgtcgtctgcgtcgtcgtaccggtctgagtgatcgtccgctgagccatgcaatggatgatctgcgtccggctggtccggcagaatatcgttggctgctggcagttcaggaacgtgaacgtcgtctgctggcacaggcagttcatgatgatattggtcagtatctgaccggtattcgtacccagttactgctgctgcgttgtacagccggtgatggtcagcctgcactggcacgtctggaacgttattgtcagggtctggatgatagctttcgtgcactgctgcgtggtctgcctccgcctgcactgcaacgtcagagcctggatgaagcactgcgtgcatgtgttgcagattggcaggaaggtcagggtattgcctgtgaactgcgtctgggtggtccgctgcctccgctggatgcagcagcaggcacccatctgtatcgtctgctgcaagaagcactgaataacgttgcacgtcatgccggtgcaagccgtgttcgtattcgtctgcaatgtcgtggtggttttctgtatctgagcgttcgtgatgatggtcgtggttgtgcagcagatccggcaccgggttttggtctggatagtatgcgtcagcgtgcacgttgtctgggtgcagaactgagcctgcgtagccgtccgggtcagggcctggcactgcgtctgcgtatgcctctggaacgtgatgattaatactagagaggaggaaataaaaatgaaaattctgctggttgatgatcattttgtggttcgtgaaggtctggcagcactgctgcgtggtctgctgccggatatggaagttaatgaagccggtgatggtgaagaagcactgcaagcagttcagcgtgaaattccgagcctggttattgttgatctgggtctgcctggtattagcggtctggaactgacccgtcgtctgcgtcagcgtctgccgcaactgcgtgttctgttttttagcctgcatgatgaactggcactggttcgtcaggcactggatgccggtgcacgtggttatgttaccaaacgtgcagcaccgaccgttctgctggaagcaattcgtcgtgttctggcaggtcaactgtatctggaacagccgctggcaacccgtctggcatgtcagagctgggaagaacagggtggtgcagcactgcgtggcctgacccgtcgcgaatttgaaatttttcgtctgctggcacgtggtctggcactgcgtgaagttgcagaacatctgggtgttagcgcaaaaaccgttagcaatcatgttagcctgctgaaacagaaactgcaagttagcacccaggcagaactggttcatcgtgccattgatagcggtgttctgcgcctgggactgccgctgccgctggcagaagaagcacgcggtagcctgggttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z