LOCUS       BBa_K634003             2427 bp    DNA     linear       29-Nov-2024
DEFINITION  exaDE-GFP
ACCESSION   BBa_K634003
VERSION     BBa_K634003.1  
FEATURES             Location/Qualifiers
     RBS             9..22
                     /label=RBS
     CDS             23..841
                     /label=exaD
     CDS             1..841
                     /label=BBa_K634000
     RBS             850..863
                     /label=rbs
     CDS             864..1541
                     /label=exaE
     CDS             850..1541
                     /label=BBa_K634001
     misc_feature    1..1541
                     /label=BBa_K634002
     RBS             1550..1564
                     /label=RBS-1\Strong
     RBS             1557..1561
                     /label=RBS
     misc_feature    1550..1564
                     /label=BBa_B0030
     RBS             1550..1564
                     /label=BBa_B0030
     CDS             1571..2290
                     /label=GFP protein
     CDS             1571..2290
                     /label=BBa_E0040
     stem_loop       2310..2353
                     /label=stem_loop
     misc_feature    2299..2378
                     /label=BBa_B0010
     terminator      2299..2378
                     /label=BBa_B0010
     stem_loop       2394..2413
                     /label=T7 TE
     misc_feature    2420..2420
                     /label=stop
     polyA_site      2414..2427
                     /label=polya
     misc_feature    2387..2427
                     /label=BBa_B0012
     terminator      2387..2427
                     /label=BBa_B0012
     misc_feature    1550..2427
                     /label=BBa_E0840
ORIGIN
        1 gtactagaag gaggaaataa aaatggatcg tagccgtcgt catgcacatc gtcgtcatcc
       61 gggtgttgca catccggcag atccgcagcc tgcacgtgtt cgttttccgc tgtttccgcc
      121 tccgcgtccg ggtaaaacct gtcgtctgcg tcgtcgtacc ggtctgagtg atcgtccgct
      181 gagccatgca atggatgatc tgcgtccggc tggtccggca gaatatcgtt ggctgctggc
      241 agttcaggaa cgtgaacgtc gtctgctggc acaggcagtt catgatgata ttggtcagta
      301 tctgaccggt attcgtaccc agttactgct gctgcgttgt acagccggtg atggtcagcc
      361 tgcactggca cgtctggaac gttattgtca gggtctggat gatagctttc gtgcactgct
      421 gcgtggtctg cctccgcctg cactgcaacg tcagagcctg gatgaagcac tgcgtgcatg
      481 tgttgcagat tggcaggaag gtcagggtat tgcctgtgaa ctgcgtctgg gtggtccgct
      541 gcctccgctg gatgcagcag caggcaccca tctgtatcgt ctgctgcaag aagcactgaa
      601 taacgttgca cgtcatgccg gtgcaagccg tgttcgtatt cgtctgcaat gtcgtggtgg
      661 ttttctgtat ctgagcgttc gtgatgatgg tcgtggttgt gcagcagatc cggcaccggg
      721 ttttggtctg gatagtatgc gtcagcgtgc acgttgtctg ggtgcagaac tgagcctgcg
      781 tagccgtccg ggtcagggcc tggcactgcg tctgcgtatg cctctggaac gtgatgatta
      841 atactagaga ggaggaaata aaaatgaaaa ttctgctggt tgatgatcat tttgtggttc
      901 gtgaaggtct ggcagcactg ctgcgtggtc tgctgccgga tatggaagtt aatgaagccg
      961 gtgatggtga agaagcactg caagcagttc agcgtgaaat tccgagcctg gttattgttg
     1021 atctgggtct gcctggtatt agcggtctgg aactgacccg tcgtctgcgt cagcgtctgc
     1081 cgcaactgcg tgttctgttt tttagcctgc atgatgaact ggcactggtt cgtcaggcac
     1141 tggatgccgg tgcacgtggt tatgttacca aacgtgcagc accgaccgtt ctgctggaag
     1201 caattcgtcg tgttctggca ggtcaactgt atctggaaca gccgctggca acccgtctgg
     1261 catgtcagag ctgggaagaa cagggtggtg cagcactgcg tggcctgacc cgtcgcgaat
     1321 ttgaaatttt tcgtctgctg gcacgtggtc tggcactgcg tgaagttgca gaacatctgg
     1381 gtgttagcgc aaaaaccgtt agcaatcatg ttagcctgct gaaacagaaa ctgcaagtta
     1441 gcacccaggc agaactggtt catcgtgcca ttgatagcgg tgttctgcgc ctgggactgc
     1501 cgctgccgct ggcagaagaa gcacgcggta gcctgggtta atactagaga ttaaagagga
     1561 gaaatactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga
     1621 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc
     1681 aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg
     1741 gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca
     1801 tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac
     1861 tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga
     1921 tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct
     1981 tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca
     2041 aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca
     2101 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga
     2161 caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca
     2221 catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata
     2281 caaataataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     2341 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
     2401 cttcgggtgg gcctttctgc gtttata
//